Browse > Article

Anti-oxidant and Anti-aging Activities of Essential Oils of Pinus densiflora Needles and Twigs  

Kim, Youn-Joung (Division of Pathophysiology College of Pharmacy Chung-Ang University)
Cho, Bong-Jae (Division of Pathophysiology College of Pharmacy Chung-Ang University)
Ko, Myung-Soo (Division of Pathophysiology College of Pharmacy Chung-Ang University)
Jung, Jae-Min (Division of Pathophysiology College of Pharmacy Chung-Ang University)
Kim, Hee-Rae (Division of Pathophysiology College of Pharmacy Chung-Ang University)
Song, Ho-Sun (Division of Pathophysiology College of Pharmacy Chung-Ang University)
Lee, Ji-Yun (Division of Pathophysiology College of Pharmacy Chung-Ang University)
Sim, Sang-Soo (Division of Pathophysiology College of Pharmacy Chung-Ang University)
Kim, Chang-Jong (Division of Pathophysiology College of Pharmacy Chung-Ang University)
Publication Information
YAKHAK HOEJI / v.54, no.4, 2010 , pp. 215-225 More about this Journal
Abstract
To investigate effects of pine essential oil isolated by steam distillation from Pinus densiflora needles (PN) and twigs (PT) on anti-oxidant and anti-aging activities, the experiments were carried out to determine anti-oxidant and antiaging activities on DPPH radical scavenging activity, NBT/xanthine oxidase-superoxide scavenging activity, silica-induced intracellular $H_2O_2$ and hydroxyl radical generation in RAW264.7 cells, hyaluronidase and elastase activities in vitro. Essential oil of PN and PT were contained 0.225 and 0.176 (w/v) %, respectively. PN was contained with 30 kinds of essential oil and its major constituent is $\alpha$-pinene (21.5%). Further PT was contained with 40 kinds of essential oil and its major constituent is $\beta$-pinene (22.4%) in GC/MS assay. Other essential oils of PN were $\beta$-pinene, camphene, myrcene, limonene, terpinolene, $\alpha$-terpineol, bornyl acetate, $\alpha$-copaene, caryophellene and humulene and PT were $\alpha$-pinene, camphene, phellandrene, limonene, terpinolene, $\alpha$-terpineol, bornyl acetate, $\alpha$-copaene, caryophellene and humulene. The essential oil of PT have more active than that of PN in anti-oxidant activity which has significant DPPH radical and superoxide scavenging activity, and significant inhibitory activities on silica-induced intracellular $H_2O_2$ and hydroxyl radical generation, as well as, significantly inhibited elastase and hyaluronidase activities. Further, phellandrene, myrcene and $\alpha$-pinene have DPPH radical and superoxide scavenging activities, $\beta$-pinene, terpinolene, myrcene and phellandrene inhibited silica-induced intracellular $H_2O_2$ and hydroxyl radical generations. And also phellandrene and $\beta$-pinene inhibited hyaluronidase and elastase activities. In conclusion, the essential oils isolated from PN and PT have anti-oxidant and anti-aging activities.
Keywords
pinus essential oil; superoxide; hydroxyl radical; hyaluronidase;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Muhlbauer, R. C., Lozano, A., Palacio, S., Reinli, A. and Felix, R. : Common herbs, essential oils, and monoterpenes potently modulate bone metabolism. Bone 32, 372 (2003).   DOI   ScienceOn
2 Huang, H. M., Zhang, H., Ou, H. C., Chen, H. L., Gibson, G. E. : alpha-Keto-beta-methyl-n-valeric acid diminishes reactive oxygen species and alters endoplasmic reticulum Ca(2+) stores. Free Radic. Biol. Med. 37, 17 (2004).
3 Choi, J. H., Kim, D. I., Park, S. H., Back, S. J., Kim, N. J., Cho, W. K., Kim, K. J. and Kim, H. S. : Effects of pine needle ethyl acetate fraction on acetylcholine (ACh) and its related enzymes in brain of rats. Kor. J. Nutr. 37, 95 (2004).
4 Mumm, R., Tiemann, T., Schulz, S. and Hilker, M. : Analysis of volatiles from black pine (Pinus nigra): significance of wounding and egg deposition by a herbivorous sawfly. Phytochem. 65, 3221 (2004).   DOI   ScienceOn
5 Jang, M. J., An, B. J., Lee, C. E., Lee, B. G. and Lee, D. H. : Study on the anti-oxidant effect of Pinus rigida Mill. inner bark extracts. J. Kor. For. Soc. 97, 88 (2008)
6 Koukos, P. K., Papadopoulou, K. I., Patiaka, D. T. and Papagiannopoulos, A. D. : Chemical composition of essential oils from needles and twigs of balkan pine (Pinus peuce grisebach) grown in Northern Greece. J. Agric. Food Chem. 48, 1266 (2000).   DOI   ScienceOn
7 Burits, M. and Bucar, F. : Antioxidant activity of Nigella sativa essential oil. Phytother. Res. 14, 323 (2000).   DOI   ScienceOn
8 Grassmann, J., Hippeli, S., Dornisch, K., Rohnert, U., Beuscher, N. and Elstner, E. F. : Antioxidant properties of essential oils. Possible explanations for their anti-inflammatory effects. Arzneimittelforschung 50, 135 (2000).
9 Hong, E. J., Na, K. J., Choi, I. G., Choi, K. C. and Jeung, E. B. : Antibacterial and antifungal effects of essential oils from coniferous trees. Biol. Pharm. Bull. 27, 863 (2004).   DOI   ScienceOn
10 Jung, M. J., Chung, H. Y., Choi, J. H. and Choi, J. S. : Antioxidant principles from the needles of red pine, Pinus densiflora. Phytother. Res. 17, 1064 (2003).   DOI   ScienceOn
11 Ando, Y. : Breeding control and immobilizing effects of wood microingredients on house dust mites. Nippon Koshu Eisei Zasshi. 41, 741 (1994).
12 Kim, S. M., Cho, Y. S., Sung, S. K., Lee, I. G., Lee, S. H. and Kim, D. G. : Antioxidative and nitrite scavenging activity of pine needle and green tea extracts. Kor. J. Food Sci. Anim. Resour. 22, 13 (2002).   과학기술학회마을
13 Choi, E. M. : Antinociceptive and antiinflammatory activity of pine (Pinus densflora) pollen extract. Phytother. Res. 21, 471 (2007).   DOI   ScienceOn
14 Li, Q., Kobayashi, M., Inagaki, H., Hirata, Y., Li, Y. J., Hirata, K., Shimizu, T., Suzuki, H., Katsumata, M., Wakayama, Y., Kawada, T., Ohira, T., Matsui, N. and Kagawa, T. : A day trip to a forest park increases human natural killer activity and the expression of anti-cancer proteins in male subjects. J. Biol. Regul. Homeost Agents 24, 157 (2010).
15 Ciesla, D. J., Moore, E. E., Biffl, W. L., Gonzalez, R. J. and Silliman, C. C. : Hypertonic saline attenuation of the neutrophil cytotoxic response is reversed upon restoration of normotonicity and reestablished by repeated hypertonic challenge. Surgery 129, 567 (2001).
16 Jacob, C., Arteel, G. E., Kanda, T., Engman, L. and Sies, H. : Water-soluble organotellurium compounds: catalytic protection against peroxynitrite and release of zinc from metallothionein. Chem. Res. Toxicol. 13, 3 (2000).   DOI   ScienceOn
17 Lazutka, J. R., Mierauskiene, J., Slapsyte, G. and Dedonyte, V. : Genotoxicity of dill (Anethum graveolens L.), peppermint (Menthaxpiperita L.) and pine (Pinus sylvestris L.) essential oils in human lymphocytes and Drosophila melanogaster. Food Chem. Toxicol. 39, 485 (2001).   DOI   ScienceOn
18 Macchioni, F., Cioni, P. L., Flamini, G., Morelli, I., Perrucci, S., Franceschi, A., Macchioni, G. and Ceccarini, L. : Acaricidal activity of pine essential oils and their main components against Tyrophagus putrescentiae, a stored food mite. J. Agric. Food Chem. 50, 4586 (2002).   DOI   ScienceOn
19 McCune, L. M. and Johns, T. : Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the Indigenous Peoples of the North American boreal forest. J. Ethanopharmacol. 82, 197 (2002).   DOI   ScienceOn
20 Berrabah, M., Andre, D., Verite, P., Zahidi, A. and Lafont, O. : Aminoglutethimide included in nanocapsules suspension: comparison of GC-MS and HPLC methods for control. J. Pharm. Biomed Anal. 35, 761 (2004).   DOI   ScienceOn
21 Mariencheck, M. C., Davis, E. C., Zhang, H., Ramirez, F., Rosenbloom, J., Gibson, M., Parks, W. C. and Mecham, R. P. : Fibrillin-1 and fibrillin-2 show temporal and tissue-specific regulation of expression in developing elastic tissues. Connect Tissue Res. 31, 87 (1995).   DOI   ScienceOn
22 Yoshihiro, O., Shingo, T., Minoru, A., Akira, I., Ryoji, K. and Izumi, H. : Expression of elastin-related proteins and matrix metalloproteinases in actinic elastosis of sun-damaged skin. Arch. Dermatol. Res. 292, 27 (2000).   DOI   ScienceOn
23 Choi, H. S., Hang, D., Kang, S. C., Sohn, E. S., Lee, S. P., Pyo, S. K. and Son, E. W. : Immunomodulatory activity of pine needle (Pinus densflora) extracts in macrophages. J. Food Sci. Nutr. 11, 105 (2006).   과학기술학회마을   DOI   ScienceOn
24 Jeon, J. R., Kim, J. Y., Lee, K. M. and Cho, D. H. : Anti-obese effects of mixture contained pine needle, black tea, and green tea extracts. J. Kor. Soc. Appl. Biol. Chem. 48, 375 (2005).
25 Yoo, J. H., Cha, J. Y., Jeong, Y. K., Chung, K. T. and Cho, Y. S. : Antioxidative effects of pine (Pinus densflora) needle extracts. J. Life Sci. 14, 863 (2004).   DOI   ScienceOn