• Journal of Food Hygiene and Safety
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• v.8 no.2
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• pp.85-90
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• 1993

The aglycones of vicine and convicine have been implicated as the causative factors for favism in fababeans. Thus, the presence of these compounds in fababeans is a potential obstacle to the use of fababeans and fababean preparations in foods. Investigations of vicine stability in fababeans and in pure solutions revealed that vicine is very stable, however, divicine derived from vicine by ${\beta}-gIucosidase$ action or by acid hydrolysis is highly unstable at conditions prevalent in food processing. Therefore, the possibility of incorporating of ${\beta}-gIucosidase$ in food products containing fababean preparations was suggested to overcome potential risks of favism related to the consumption of fababean fortified products.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.189-195
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• 1999

Isoflavones of soy milk were mainly present as sugar conjugates such as genistin and daidzin which a glucosyl residue was attached to their aglycones, genistein and daidzein through ${\beta}-glycosidic$ bond, respectively. When soy milk containing sucrose as a sugar source was fermented with lactic acid bacteria, small amount of lactic acid $(0.16{\sim}0.29%)$ was produced but isoflavone conjugates were fully hydrolyzed. Supplementation of glucose or lactose was required for normal lactic acid production and affected the hydrolysis of isoflavone conjugates in some lactic acid bacteria. In the case of Lactobacillus delbrueckii subsp. delbrueckii KCTC 1047, glycosidic bond of isoflavone was fully hydrolyzed regardless of glucose supplementation. But only $25{\sim}40%$ of daidzin and $65{\sim}80%$ of genistin was hydrolyzed when glucose was added into soy milk in the other lactic acid bacteria, Lactobacillus bulgaricus KCTC 3188, Lactobacillus casei KCTC 3109, Lactobacillus delbrueckii subsp lactis KCTC 1058, Lactobacillus lactis KCTC 2181. The hydrolyzing enzyme, ${\beta}-glucosidase$ produced by lactic acid bacteria except Lactobacillus delbrueckii subsp. delbrueckii KCTC 1047 could be considered as inducible in the fermentation of soy milk and its production was decreased when glucose was added.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.5
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• pp.671-675
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• 2008

Based on information about the major microbial composition of kimchi and its relation to the taste, Leuconostoc mesenteroides K2M5 and Lactobacillus sakei K5M3 were selected as kimchi starter candidates. These two strains were found to be safe for industrial use because they showed neither harmful characteristics like ${\beta}$-hemolysis, ammonia and indole formation, and gelatin liquefaction, nor enzymatic activities like phenylalanine deaminase, ${\beta}$-glucuronidase, ${\beta}$-glucosidase, 7${\alpha}$-dehydroxylase and nitroreductase. Starter kimchi made with these strains were better in taste than the conventional kimchi when they are evaluated both by laboratory personnel and the public. Also microbial analysis of starter kimchi showed only starter bacteria after they were fermented to have the optimum acidity. Starter kimchi prepared with these two strains were not much different in physicochemical properties to the conventional kimchi except in that the starter kimchi were much higher in volatile organic acid content such as lactic acid. These results suggest that kimchi quality can be controlled to have consistent properties, both in taste and microbial composition, by using bacterial starters.

• Archives of Pharmacal Research
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• v.20 no.5
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• pp.443-447
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• 1997

As the growth factor of lactic acid bacteria, LD (trehalose) was isolated from Lentinus edode5 by using silica gel column chromatography. LD induced the growth of Bifidobacteria breve and Lactobacillus brevis, which were isolated from human feces. LD selectively induced the growth of lactic acid bacteria among total microflora. When total intestinal microflora were cultured in the medium containing LD, it stimulated the growth of lactic acid bacteria and inhibited harmful enzymes, ${\beta}$-glucosidase, ${\beta}$-glucuronidase, and tryptophanase, of intestinal bacteria. LM, which was a monosaccharide from L. edooles, induced the growth of lactic acid bacteria but it seems to be invaluable in vivo. LH isolated from L. edodes by Sephadex G-100 column chromatography was not effective for the growth of lactic acid bacteria.

• 한국신재생에너지학회:학술대회논문집
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• 2008.05a
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• pp.225-228
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• 2008

This study shows that lignocellulosic biomass saccharification work has been carried out with rice-straw by the extracellular enzyme from KMU001, and the enzymes produced in 5%(w/v) wood biomass were characterized by protein and various enzyme activity measurements. Several cellulases such as Endoglucanase(EG), $\beta$-D-1,4-Glucosidase(BGL), Cellobiohydrolase(CBH), and $\beta$-D-1,4-Xylanase (BXL) were detected. Saccharification of rice-straw by the enzyme yielded about 233mg/g of glucose after 48hrs.

• Korean Journal of Pharmacognosy
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• v.25 no.3
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• pp.233-237
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• 1994

Bacillus P-92 which fermented antler was isolated from intestinal bacteria. The biological activites, carbon clearance and growth activity of lactic acid bacteria, of fermented antler was better than those of untreated antler. The enyzmes activities, ${\beta}-glucosidase,\;{\beta}-glucuronidase$ and tryptophanase, of intestinal bacteria of mice treated with fermented antler were lower than those of mice treated with untreated antler, although those of mice treated with fermented antler or untreated antler were higher than those of control. Biological activity of the antler seems to be increased by fermentation.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.04a
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• pp.84-84
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• 1997

장내세균은 음식물, 스트레스, 생활환경에 의해 영향을 받으며 그 결과 장내 세균이 생산하는 효소활성도 영향을 받는다. 장내미생물효소는 질병과 밀접한 관계를 갖고 있으며 장내의 pH와 효소저해제에 의해 영향을 받는 데 장내의 높은 pH에 의해 $\beta$-glucosidase, $\beta$-glucuronidase, tryptophanase 등의 장내유해효소활성이 유도되므로 장내의 pH를 낮춤으로써 효소활성을 저하시킬 수 있다. Bifidobacterium은 장내에서 lactic acid, acetic acid를 생산하여 장내의 pH를 낮추며 유해균의 증식을 억제하고 유해효소의 활성을 억제하는 역할을 할 것으로 기대된다. 특히 한국인으로부터 분리된 유산균일 경우 한국인의 장내에 가장 잘 정착되며 장내미생물의 유해효소를 효과적으로 억제할 것으로 생각된다.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.371-374
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• 2005

In our previous work in transcriptional regulation of sugar, expression of genes encoding putative glycosyl hydrolases in Arabidopsis was induced by sugar starvation. They were annotated as b-galactosidase (At5g56870), ${\beta}-xylosidase$ (At5g49360) and ${\beta}-glucosidase$ (At3g60140), which belong to glycosyl hydrolase family that has a catalytic domain of polysaccharides. From the primary structure of deduced amino acid sequence, they were predicted to localize to cell wall. Further investigation of these cell wall hydrolases implicated that cell wall polysaccharides provide metabolizable sugars to nutrient allocation under sugar starvation.

• Macromolecular Research
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• v.16 no.1
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• pp.1-5
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• 2008

The enzymatic hydrolysis of chitin has been studied for almost a century, and early work established that at least two enzymes are required, a chitinase that mainly yields the disaccharide N,N'-diacetylchitobiose, or $(GlcNAc)_2$, and a "chitobiase", or ${\beta}$-N-acetylglucosaminidase, which gives the final product G1cNAc. This pathway has not been completely identified but has remained the central concept for the chitin catabolism through the $20^{th}$ century1 including in marine bacteria. However, the chitin catabolic cascade is quite complex, as described in this review. This report describes three biologically functional genes involved in the chitin catabolic cascade of Vibrios in an attempt to better understand the metabolic pathway of chitin.

• YAKHAK HOEJI
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• v.39 no.1
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• pp.55-60
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• 1995

The acetone precipitates of the hot water extract of Torilis ftuctus showed strong hemostatic activity which was not inhibited by aspirin. This activity was not through platelet activation but possibly through activating some coagulation factors in plasma. The hemostatic action of the precipitates was not active by oral adminitration and no behavioral toxicity was appeared in treated mice. However, mice treated with the acetone precipitates through tail vein showed serious tremor and then were killed probably by the thrombus produced in the body. The hemostatic activity was still remained after treatment with $\beta$-glucosidase, $\beta$-alactosidase, $\alpha$-amylase, subtilisin BPN', or trypsin but completely lost by acid hydrolysis. The active components seemed to be a complex of unidentified macromolecules to which some phenolic compounds were strongly bound.