• Journal of dental hygiene science
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• v.15 no.6
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• pp.794-799
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• 2015

In recent years, there has been a global trend toward the importance of natural extracts for the prevention and treatment of human diseases. ${\beta}$-glucan is known to have anti-inflammatory activity, anti-cancer, and improvement of immune system. Polycan is purified ${\beta}$-glucan from Aureobasidum pullulans SM-2001. The anti-cancer effects of ${\beta}$-gluconsan calcium, polycan and calcium gluconate complex, were evaluated in human oral cancer YD-10B cells. YD-10B cells were cultured in the presence of 0, 0.5, 0.75, 1 mg/ml ${\beta}$-gluconsan calcium for 48 hours. MTT assay, cell counting, and observation of cell morphology were conducted. The number of cells decreased and cell morphology changed in the 0.5 mg/ml of ${\beta}$-gluconsan calcium. Almost all cells were dead in the 0.75 and 1 mg/ml. MTT assay showed a dose-dependent reduction in cell proliferation (p<0.05). These results indicate that ${\beta}$-gluconsan calcium exhibiting anti-cancer effects in YD-10B cells through changes in cell morphology and cell death.