Journal of dental hygiene science (치위생과학회지)

The Korean Society of Dental Hygiene Science (한국치위생과학회)
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The Anti-Cancer Effect of β-Gluconsan Calcium on Oral Cancer Cell

β-Gluconsan Calcium이 구강암 세포의 성장에 미치는 영향

  • Kim, Ji-Hye (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Jung, Yun-Sook (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Kim, Hye-Young (Department of Dental Hygiene, Kangwon National University) ;
  • Lee, Young-kyun (Department of Oral Biochemistry, School of Dentistry, Kyungpook National University) ;
  • Kim, Jae-Young (Department of Oral Biochemistry, School of Dentistry, Kyungpook National University) ;
  • Choi, Youn-Hee (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Song, Keun-Bae (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
  • 김지혜 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 정윤숙 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 김혜영 (강원대학교 치위생학과) ;
  • 이영균 (경북대학교 치의학전문대학원 구강생화학교실) ;
  • 김재영 (경북대학교 치의학전문대학원 구강생화학교실) ;
  • 최연희 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 송근배 (경북대학교 치의학전문대학원 예방치과학교실)
  • Received : 2015.10.24
  • Accepted : 2015.11.24
  • Published : 2015.12.31
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Abstract

In recent years, there has been a global trend toward the importance of natural extracts for the prevention and treatment of human diseases. ${\beta}$-glucan is known to have anti-inflammatory activity, anti-cancer, and improvement of immune system. Polycan is purified ${\beta}$-glucan from Aureobasidum pullulans SM-2001. The anti-cancer effects of ${\beta}$-gluconsan calcium, polycan and calcium gluconate complex, were evaluated in human oral cancer YD-10B cells. YD-10B cells were cultured in the presence of 0, 0.5, 0.75, 1 mg/ml ${\beta}$-gluconsan calcium for 48 hours. MTT assay, cell counting, and observation of cell morphology were conducted. The number of cells decreased and cell morphology changed in the 0.5 mg/ml of ${\beta}$-gluconsan calcium. Almost all cells were dead in the 0.75 and 1 mg/ml. MTT assay showed a dose-dependent reduction in cell proliferation (p<0.05). These results indicate that ${\beta}$-gluconsan calcium exhibiting anti-cancer effects in YD-10B cells through changes in cell morphology and cell death.

이번 연구에서는 천연추출물인 ${\beta}$-gluconsan calcium이 구강암 세포의 증식과 사멸에 미치는 영향을 평가하고자 하였으며, 다음과 같은 결론을 얻었다. ${\beta}$-gluconsan calcium 0.5 mg/ml 처리군에서 세포수의 감소와 형태 변화가 관찰되었으며, 0.75, 1 mg/ml에서는 거의 모든 세포가 사멸하였다. ${\beta}$-gluconsan calcium을 농도별로 처리한 결과, YD-10B 세포수가 농도 의존적으로 감소하였으며(p<0.05), MTT 분석 결과, ${\beta}$-gluconsan calcium 처리한 군은 아무것도 처리하지 않은 군에 비해 모두 세포 활성이 저하되었다(p<0.05). 이상의 결과들을 종합해 보았을 때, polycan과 calcium gluconate 복합제인 ${\beta}$-gluconsan calcium은 구강암 세포의 성장 억제에 효과가 있는 것으로 생각된다.

Keywords

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