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http://dx.doi.org/10.17135/jdhs.2015.15.6.794

The Anti-Cancer Effect of β-Gluconsan Calcium on Oral Cancer Cell  

Kim, Ji-Hye (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
Jung, Yun-Sook (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
Kim, Hye-Young (Department of Dental Hygiene, Kangwon National University)
Lee, Young-kyun (Department of Oral Biochemistry, School of Dentistry, Kyungpook National University)
Kim, Jae-Young (Department of Oral Biochemistry, School of Dentistry, Kyungpook National University)
Choi, Youn-Hee (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
Song, Keun-Bae (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
Publication Information
Journal of dental hygiene science / v.15, no.6, 2015 , pp. 794-799 More about this Journal
Abstract
In recent years, there has been a global trend toward the importance of natural extracts for the prevention and treatment of human diseases. ${\beta}$-glucan is known to have anti-inflammatory activity, anti-cancer, and improvement of immune system. Polycan is purified ${\beta}$-glucan from Aureobasidum pullulans SM-2001. The anti-cancer effects of ${\beta}$-gluconsan calcium, polycan and calcium gluconate complex, were evaluated in human oral cancer YD-10B cells. YD-10B cells were cultured in the presence of 0, 0.5, 0.75, 1 mg/ml ${\beta}$-gluconsan calcium for 48 hours. MTT assay, cell counting, and observation of cell morphology were conducted. The number of cells decreased and cell morphology changed in the 0.5 mg/ml of ${\beta}$-gluconsan calcium. Almost all cells were dead in the 0.75 and 1 mg/ml. MTT assay showed a dose-dependent reduction in cell proliferation (p<0.05). These results indicate that ${\beta}$-gluconsan calcium exhibiting anti-cancer effects in YD-10B cells through changes in cell morphology and cell death.
Keywords
Anti-cancer; ${\beta}$-gluconsan calcium; Oral cancer cells;
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