• 한국미생물·생명공학회지
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• 제19권5호
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• pp.444-449
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• 1991

Pullulanase의 역반응능을 이용하여 maltose와 $\beta$-cyclodextrin으로부터 maltosyl-$\beta$-cyclodextrin을 중합합성하기 위한 최적효소반응조건을 검토하였다. Maltose와 $\beta$-CD를 기질로 maltosyl-$\beta$-CD을 합성하였을 경우, 기질의 농도 70( w/w, 70g/100ml $H2_O$ ), malto-loigo당 /$\beta$-CD의 혼합비 12.7, 그리고 사용효소량 350 units/100ml일 때 최대전융인 43(w/w, g branched-CD/g CD)를 얻었고, 생성량은 2.31g/100ml였다. Maltosyl-$\beta$-CD의 효소합성의 적정 pH 및 온도는 각각 4.9와 $60^{\circ}C$ 였다. 또한 maltose와 $\alpha ,\beta$-그리고 $\gamma$-CD 각각을 기질로하여 maltosyl $\Alpha, \beta$ 그리고 $\gamma$-CD를 합성하였을 경우 전환율은 51.8, 42.6, 그리고 48.1로써, 생성량은 각각 2.8, 2.3 그리고 2.6g/100ml였다.

• 한국식품저장유통학회지
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• 제12권3호
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• pp.247-251
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• 2005

아가리쿠스버섯의 약효성분으로 고형 음용차를 제조하기 위하여 건 버섯을 열수추출, 에탄올 침전, 투석, 동결건조 등의 처리를 행하여 단백다당체를 분리하고 부형제로 dextrin(DE=9, DE=23) 및 ${\beta}$-cyclodextrin(CD)을 각각 첨가하여 분무건조한 후 압출하여 과립을 제조한 다음 부형제의 종류에 따른 과립의 품질특성을 조사 비교하였다. 과립의 수분함량은 DE=9 첨가구에서 가장 높았고, 총당 함량은 ${\beta}$-CD, DE=23, DE=9 첨가구 순이었으나, pH와 단백질함량은 부형제에 따른 유의적인 차이를 보이지 않았다. L값은 DE=9, ${\beta}$-CD, DE=23 첨가구 순이었고, -a값은 부형제간 거의 차이가 없었으며, b값은 ${\beta}$-CD 첨가구에서 가장 높게 나타났다. 과립의 용해도는 DE=9보다 DE=23과 ${\beta}$-CD 첨가구에서 높았으며 두 종류의 부형제간 차이는 보이지 않았다. 흡습성은 ${\beta}$-CD 첨가구에서 가장 크게 나타났으며 DE=9 첨가구에서 가장 적게 나타났다. 관능검사 결과 종합적인 기호도는 ${\beta}$-CD가 가장 우수하였다.

• Food Science and Biotechnology
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• 제18권1호
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• pp.185-189
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• 2009

Microencapsulation of flavor was carried out by molecular inclusion process using $\beta$-cyclodextrin (${\beta}CD$). ${\beta}CD$-flavor complex was prepared at various flavor-to-${\beta}CD$ ratios (1:6-1:12) to determine the effect of ${\beta}CD$ concentration on the inclusion efficiency. Maximum total oil retention and minimal surface oil content were obtained at flavors to ${\beta}CD$ ratio of 1:10. The physical properties and controlled release pattern of flavors from ${\beta}CD$-flavor complex were measured and compared with spray-dried microcapsules prepared using carbohydrate wall system. ${\beta}CD$-flavor complex showed higher total oil retention and surface oil contents, smaller mean particle size, lower moisture uptake, and higher oxidation stability than spray-dried microcapsule. Oxidative stability of flavor was correlated with hygroscopicity of wall materials. The controlled release mechanism was highly affected by temperature and characteristics of wall materials.

• 한국환경보건학회지
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• 제28권5호
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• pp.65-70
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• 2002

Recently, surfactants were frequently used in order to desorb the hydrophobic organic compounds (HOCs) from soil and to enhance the bioavailability. Among them, -cyclodextrin ($\beta$-CD) is one of those. This study was performed to investigate the binding characteristics between benzene and $\beta$-CD and to examine the bioavailability of benzene. First, we investigated binding characteristics between benzene and $\beta$-CD in water and water/soil system. Then, we examined the effect of $\beta$-CD on the biodegradation of benzene in water and water/soil system. Experimental results on the binding characteristics showed that $\beta$-CD resulted in an efficient complex formation with benzene. As -CD concentration increased, the benzene concentration complexed with $\beta$-CD rapidly increased to 30-40% initial benzene added, and reached the equilibrium. We also investigated the effect of $\beta$-CD on the desorption of benzene from soil in the water/soil system. As $\beta$-CD concentration increased, benzene concentration desorbed into water increased up to 90%. How-ever, in its application to biodegradation of benzene in water and water/soil system, the biodegradation rate of benzene did not improved in the presence of $\beta$-CD compared with in the absense of $\beta$-CD. This result indicated that $\beta$-CD was more preferentially used as a carbon source than benzene. Therefore, for remediation of benzene contaminated soils, $\beta$-CD can be used as a surfactant to desert benzene from soil, and then ex-situ chemical treatment can be applied for the remediation.

• 약학회지
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• 제37권4호
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• pp.331-340
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• 1993

Inclusion complex of omeprazole(OMZ) with hydroxypropyl-$\beta$-cyclodextrin(HP-$\beta$-CD) was prepared by freeze-drying method. The complexation was confirmed by means of UV, CD, IR, DSC, XRD and $^{1}$H-NMR spectra. The benzimidazole moiety of OMZ might be found to be included into the cavity of HP-$\beta$-CD and the inclusion complex appeared to be $A_{L}$ type. The stoichiometric ratio of OMZ : HP-$\beta$-CD was found to be 1:1, and the stability constants of the inclusion complex by solubility and UV method were about 34 and 45 M$^{-1}$, respectively. The dissolution of OMZ was significantly enhanced from powder and. yablet of OMZ-HP-$\beta$-CD complex when compared to those of OMZ alone, and oil-to-water partition coefficient of OMZ-HP-$\beta$-CD complex was significantly higher than that of OMZ alone. Therefore, it was expected that the bioavailability of OMZ could be improved markedly by inclusion complexation of OMZ with HP-$\beta$-CD.

• 한국식품과학회지
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• 제29권2호
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• pp.302-308
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• 1997

Epichlorohydrin을 이용하여 ${\beta}-CD$ 중합체를 제조한 후 차단분자량 10,000인 막(YM 10)을 이용하여 수용성 ${\beta}-CD$ 중합체와 불용성 ${\beta}-CD$ 중합체를 분리하였다. 최적분리 조건은 막횡단압력 51.7 kPa, 운용온도 $35^{\circ}C$, 용적농축비 10이었으며, 이때의 flux는 $0.025\;mL/cm^{2}/min$ 이었다. 겔 투과 크로마토그래피 결과 수용성 중합체의 중합도는 $2{\sim}8$, 불용성 중합체는 10 이상으로 나타났으며, 이들 ${\beta}-CD$ 중합체의 소수성 물질들과의 포접 형성능을 비교하였다. 색소물질인 4-dimethylaminoazobenzene과 methyl red를 이용하여 ${\beta}-CD$와 ${\beta}-CD$ 중합체와의 포접 능력을 측정하였다. 포접 복합체 형성 여부를 간접적으로 알 수 있는 분광학적 변화를 측정한 결과 두 색소물질 흡광도의 강도가 증가하였으며 최대 흡광도 위치가 변하였다. 감귤류의 주된 flavonoid이며 쓴맛물질인 naringin은 물에 대한 용해도가 낮으나 ${\beta}-CD$ 중합체과 포접복합체를 형성함으로써 수용성이 증가하였다. ${\beta}-CD$ 단위체보다는 ${\beta}-CD$ 중합체의 포접능력이 훨씬 강하였으며, 중합도별 포접능력에 있어 불용성 ${\beta}-CD$ 중합체와 수용성 ${\beta}-CD$ 중합체간에 큰 차이는 나타나지 않았다. ${\beta}-CD$ 단위체는 용해도가 극히 낮아 쓴맛 물질 제거 이용에 있어 제한이 있는 반면, 수용성 ${\beta}-CD$ 중합체는 용해도가 높아 감귤류 등으로부터 쓴맛 성분을 감소시키는 공정에의 이용 가능성이 높은 것으로 나타났다.

• 청정기술
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• 제19권3호
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• pp.212-218
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• 2013

초임계 이산화탄소를 역용매로 이용하는 aerosol solvent extraction system (ASES) 방법을 사용하여 HP-${\beta}$-CD 미립자를 제조하였으며, 공정변수가 입자의 크기와 형태에 미치는 영향을 조사하였다. 또한, 초임계 이산화탄소를 이용하여 이부프로펜과 HP-${\beta}$-CD의 포접복합체를 제조하였으며, ASES 공정에 의해 변형된 HP-${\beta}$-CD의 입자 형상이 포접효율에 미치는 영향에 대해 고찰하였다. ASES 공정으로 제조된 HP-${\beta}$-CD 미립자는 50~200 nm 크기의 나노 입자들이 응집된 입자 형상을 나타내었다. 에탄올 수용액을 HP-${\beta}$-CD의 용매로 사용한 경우 구형의 입자가 제조되었으며, 물의 양이 증가함에 따라 입자의 크기가 증가하였다. 초임계 이산화탄소를 이용해 고체상태에서 이부프로펜/HP-${\beta}$-CD 포접복합체를 제조하는 경우 초임계 ASES 방법에 의한 미세입자화 공정을 통해 포접효율을 향상시킬 수 있는 가능성을 확인하였다.

• Bulletin of the Korean Chemical Society
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• 제25권2호
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• pp.216-220
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• 2004

${\beta}$-CD and CM- ${\beta}$-CD as chiral NMR shift agents were used to resolve the enantiomers of noradrenaline (NA). The stoichiometry of each complex formed between the CDs and the enantiomers of NA was found to be 1 : 1 through the continuous variation plots. The binding constants (K) of the complexes were determined from $^1H$ NMR titration curves. This result indicated that both ${\beta}$-CD and CM- ${\beta}$-CD formed the complexes with the S(+)-NA more preferentially than its R(-)-enantiomer. The K values for the complexes with ${\beta}$-CD ($K_{S(+)}$ = 537 $M^{-1}$ and $K_{R(-)}$ = 516 $M^{-1}$ was larger than those with CM- ${\beta}$-CD ($K_{S(+)}$ = 435 $M^{-1}$ and $K_{R(-)}$ = 313 $M^{-1}$), however, enantioselectivity (${\alpha}$) of S(+)- and R(-)-NA to CM- ${\beta}$-CD ( ${\alpha}$ = 1.38) was larger than that to ${\beta}$-CD ( ${\alpha}$ = 1.04), indicating that CM- ${\beta}$-CD was the better chiral NMR solvating agents for the recognition of the enantiomers of NA. Two dimensional rotating frame nuclear Overhauser enhancement spectroscopy (ROESY) experiments were also performed to explain the binding properties in terms of spatial fitting of the NA molecule into the macrocyclic cavities.

• Bulletin of the Korean Chemical Society
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• 제27권9호
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• pp.1397-1400
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• 2006

A detailed spectroscopic study ($^1H$ NMR, COSY, ROESY) of complexation of venlafaxine hydrochloride (VEN) with $\beta$-cyclodextrin ($\beta$--CD) was carried out in solution. The stoichiometry of the complex was determined to be 1 : 1 and penetration of aromatic ring into $\beta$-Cyclodextrin cavity was confirmed from primary rim side, with the help of ROESY spectral data. The structure of the venlafaxine hydrochloride-$\beta$-CD complex has been proposed. The association constant was determined to be 234 $M^{-1}$.

• Korea-Australia Rheology Journal
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• 제12권2호
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• pp.93-100
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• 2000

The interactions between methylated $\beta$-cyclodextrin (CD) and hydrophobically modified alkali-soluble associative polymers (HASE) were examined by a rheological technique. The effect of "capping" of hydrophobes by methylated $\beta$-cyclodextrin on the viscosity and modulus was evaluated. Model HASE polymers with $C_1$to $C_{20}$ alkyl hydrophobic groups ethoxylated with~10 moles of ethylene-oxide (EO 10) and at concentrations up to 3 wt% were examined. With the addition of methylated $\beta$-CD, the steady shear viscosity profiles shift from a Newtonian profile to one that display a shear-thinning characteristic. Significant "capping" of the hydrophobes occurs for HASE polymers with $C_{l2}$, $C_{16}$ and $C_{20}$ hydrophobes as reflected by the large reduction in the viscosity. However, the steady shear viscosity remains constant when the concentration of $\beta$-CD exceeds 1 wt%, suggesting that $\beta$-CD is not able to fully encapsulate the hydrophobes of the HASE polymer. The temperature variation plots indicate that the activation energy of the HASE-EO10-$C_{20}$ system and $\beta$-CD is dependent on the magnitude of the applied shear stress. These results further reinforce the hypothesis that $\beta$-CD is not able to completely remove all the hydrophobic associations.phobic associations.