• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2006년도 PAN PACIFIC CONFERENCE vol.1
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• pp.117-121
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• 2006

Virgin bleached kraft pulp, CPO (computer printout) and white ledgers are main raw materials used in tissue mills. The utilization rate of recycled fibers and virgin pulp in South Korea tissue industry are 90% and 10%, respectively. To improve brightness of printing grades the use of 'fluorescent whitening agents (FWAs)' or 'optical brightening agents (OBAs)' has been increased. When recycling these papers for tissue production, it is unavoidable that FWAs contained in recycled papers flow into tissue production lines and remain in the products. And this draws great attention from the public. This study was carried out to develop a technology for the removal of fluorescent whitening agents from recycled fibers. Enzymatic removal of FWAs was evaluated as a method to remove FWAs from the recycled fiber. The ${\alpha}-amylase$ that degrades starched used for surface sizing of fine papers and contained substantial condition is needed to prevent the re-adsorption of FWAs on fibers. The temperature of pulp suspension was another important factor affecting on FWA removal. The higher the temperature, the greater the efficiency of removing FWAs was obtained. Optimum pH and temperature for the effective removal of FWAs were suggested to be pH 8.5 and $65^{\circ}C$, respectively. The enzymatic removal of FWAs showed a great synergistic effect when proper control in pH and temperature was made.

• Asian-Australasian Journal of Animal Sciences
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• 제22권4호
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• pp.557-564
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• 2009

Two experiments were conducted to evaluate the use of various levels of full-fat sunflower seeds (FFSS) on broiler performance and carcass characteristics. In the first experiment, FFSS was included in a basal diet at 70, 140, and 210 g/kg and the $AME_n$ values of the experimental diets were determined. The linear regression equation of $AME_n$ values on rate of inclusion was calculated. Extrapolation value for the $AME_n$ of FFSS at 100% inclusion was 14.22 MJ/kg. In the second experiment, diets containing various levels (0, 70, 140, and 210 g/kg) of FFSS were given to broilers (Ross strain) from 0 to 49 d. At 28 days of age, blood parameters and digestive enzyme activities were determined and carcass parameters were evaluated at 49 days of age. Weight gain, feed intake and feed conversion ratio (FCR) were improved (p<0.05) when broilers were fed various levels of FFSS in the starter and finisher diets. Breast, thigh, gastrointestinal tract and gizzard weight percentages were not affected by dietary treatments; however, liver weight percentage was decreased significantly (p<0.05) and weight of abdominal fat decreased but this effect was not significant. The activities of digestive enzyme (protease and ${\alpha}$-amylase) were not influenced by the treatments. Activity of alkaline phosphatase, concentrations of calcium, phosphorus, glucose, triglyceride, protein, high density lipoprotein (HDL) and low density lipoprotein (LDL) were not affected by incorporation of FFSS in the broiler diet. Although concentration of HDL increased and LDL decreased, these effects were not significant. The results of this study indicate that FFSS can be used at up to 21% in broiler diets without adverse effects on performance or other parameters of chickens.

• Preventive Nutrition and Food Science
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• 제7권2호
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• pp.174-178
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• 2002

The influence of physical forms of gelatinized rice products on enzymatic hydrolysis in vitro and glycemic and insulinemic responses in normal subjects were studied. Densities of garaedu, bagsulgi, and cooked rice were 1.20, 1.18 and 1.11 g/mL, respectively, while moisture contents of garaeduk, bagsulgi, and cooked rice were 47.5, 43.1 and 66.0% (wt.), respectively. The highest initial rate of in vitro hydrolysis by porcine pancreatic $\alpha$-amylase was observed in bagsulgi followed by cooked rice and garaeduk. However, time for complete hydrolysis seemed to reach a plateau value. Postprandial glucose and insulin responses and satiety of rice products were studied in 12 normal subjects (mean age 23.2 $\pm$ 2.4 years, 6 men and 6 women). Postprandial serum glucose and insulin levels, after consumption of the rice products, reached a peak at 30 min. Garaeduk showed significantly less incremental responses for glucose (1627.5$\pm$134.9 mg.min/dL) and insulin (2041$\pm$287.0 uU.min/mL) than did bagsulgi for glucose (2407.4$\pm$208.3 mg.min/dL) and insulin (3582$\pm$264.4 uU.min/mL). Satiety responses to the rice products were not significantly different. Therefore, it can be concluded that garaeduk may be more beneficial in controlling postprandial hyperglycemia and hyperinsulinemia than bagsulgi. These results also suggest that physical properties of starch products, acquired by the specific processing methods, affect postprandial metabolism of carbohydrate foods.

• 대한약침학회지
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• 제17권1호
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• pp.13-19
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• 2014

Objectives: The present study investigated the protective effect of Wattakaka (W.) volubilis leaf extract against streptozotocin (STZ)-induced diabetes in rats. Methods: Male Wistar rats were divided into five groups (with six rats in each group) and were fed ad libitum. The rats were fasted for sixteen hours before diabetes was induced by injecting a single dose of 90 mg/kg body weight of STZ in 0.9-percent normal saline through an intraperitoneal route. The five groups were as follows: Group 1: normal control (saline-treated), Group 2: untreated diabetic rats, Groups 3 and 4: diabetic rats treated orally with petroleum ether cold maceration extract (PEME) of W. volubilis (50 and 100 mg/kg body weight), and Group 5: diabetic rats treated orally with metformin (250 mg/kg body weight). All rats received treatment for 21 days. For the STZ-induced diabetic rats, the blood-glucose, ${\alpha}$-amylase, total protein and alanine transaminase (ALT) levels were measured on days 7, 14 and 21 of the treatment with PEME of W. volubilis and the treatment with metformin. Histopathological changes in the liver were examined with hematoxylin-eosin staining. Morphological changes in the liver were also examined with glutaraldehyde fixation. Results: The treatments with PEME of W. volubilis and with metformin in experimental rats by oral injections for 21 days produced reductions in the levels of serum biochemical markers. Histopathology and scanning electron microscopy results showed that the administrations of PEME of W. volubilis and of metformin suppressed the generation of abnormal liver cells in the STZ-treated rats. Conclusion: These results suggest that both PEME of W. volubilis and metformin have a protective effect against STZ-induced diabetes.

• 한국작물학회지
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• 제43권1호
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• pp.1-5
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• 1998

Experiments were carried out to find out the optimum water potential, temperature, and duration for the priming of rice seeds, Oryza sativa L. (cv. Ilpumbyeo) for better germination at sub-optimal temperatures. Seeds were primed in 0, -0.2, -0.4, -0.6, -0.8, and -1.0 MPa PEG (polyethylene glycol) solutions at $25^{\circ}C$. The optimum water potential for seed priming, the highest water potential at which rice seeds did not germinate, was -0.6 MPa. To find out optimum priming temperature and duration rice seeds were primed in -0.6 MPa PEG solution and 0 MPa (water as a control) for various durations at 15 and $25^{\circ}C$ and the seeds were germinated at 17, 20, and $25^{\circ}C$. Considering germination rate and speed, the optimum priming time in water (0 MPa) was 4 days at 15$^{\circ}C$ and 1 day at $25^{\circ}C$, while 4 days was the optimum priming time in a -0.6 MPa PEG solution, regardless of the priming temperature. Priming reduced the actual time of germination, especially at sub-optimal temperatures. Priming did not affect germination rate in -0.6 MPa PEG solution at 15$^{\circ}C$, but overpriming reduced the final germination rate in water at 15$^{\circ}C$ and in -0.6 PEG solution at $25^{\circ}C$. Total sugars and $\alpha$-amylase activity induced during the seed priming were negatively correlated with the final germination rate and there was no noted relationship with the speed or uniformity of germination.

• 대한약침학회지
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• 제17권3호
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• pp.7-15
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• 2014

Objectives: The present study investigated the toxic properties of petroleum ether extract of Wattakaka (W.) volubilis in Wistar female rats. Methods: An in vitro brine shrimp lethality bioassay was studied in A. Salina nauplii, and the lethality concentrations were assessed for petroleum ether extract of W. volubilis. A water soluble portion of the test extract was used in different concentrations from $100-1000{\mu}g/mL$ of 1 mg/mL stock solution. A 24-hours incubation with a 1-mL aliquot in 50 mL of aerated sea water was considered to calculate the percentage rate of dead nauplii with test extract administration against a potassium-dichromate positive control. The acute and the sub-acute toxicities of petroleum ether extract of W. volubilis were evaluated orally by using gavage in female Wistar rats. Food and water intake, body weight, general behavioral changes and mortality of animals were noted. Toxicity or death was evaluated following the administration of petroleum ether extract for 28 consecutive days in the female rats. Serum biochemical parameters, such as alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin, total cholesterol, triglyceride, total protein, glucose, urea, creatinine, sodium, potassium and ${\alpha}$-amylase levels, were measured in the toxicity evaluations. Pathological changes in isolated organs, such as the liver, kidneys, and pancreas, were also examined using hematoxylin and eosin dye fixation after the end of the test extract's administration. Results: The results of the brine-shrimp assay indicate that the evaluated concentrations of petroleum ether extract of W. volubilis were found to be non-toxic. In the acute and the sub-acute toxicity evaluations, no significant differences were observed between the control animals and the animals treated with extract of W. volubilis. No abnormal histological changes were observed in any of the animal groups treated with petroleum ether extract of W. volubilis. Conclusion: These results suggest that petroleum ether extract of W. volubilis has a non-toxic effect in Wistar female rats.

• 한국식품과학회지
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• 제35권1호
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• pp.38-43
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• 2003

흑미가루 첨가비율 증가에 따른 반죽의 물리적인 특성을 조사한 결과 흑미가루 첨가량을 증가시켰을 때 회분 함량은 증가하였고 단백질 함량과 gluten 함량은 감소하였다. farino-graph는 흑미가루 첨가량이 증가함에 따라 흡수율, 안정도, 반죽형성시간, 탄력도 및 v/v(valorimeter value)값은 감소하였고 약화도는 증가하여 반죽 시간 및 반죽의 탄력성과 신장성이 감소하였다. Amylograph는 호화개시온도, 최고점도에서의 온도는 낮았으나 최고점도 및 $94^{\circ}C$에서의 점도의 감소로 제빵시 ${\alpha}-amylase$의 활성을 높임으로써 발효능력의 향상을 가져왔다. Extensograph는 신장도, 신장저항도 및 에너지는 감소하였으나 R/E비는 높아짐으로써 제빵성은 흑미가루 첨가량이 증가할수록 낮아졌다. 상기 실험결과 farinograph와 amylograph, extensograph의 측정은 밀가루 중에 함유된 gluten의 신장성 및 신장저항성의 특성, 발효에 따른 발효력 및 가스포집력을 종합한 제빵 특성을 나타내므로 흑미가루가 혼합된 혼합분의 경우 밀가루 100% 시료보다는 단백질 함량의 저하로 제빵 특성치가 낮게 나타나 제빵 특성에 영향을 주므로 단백질원인 활성글루텐이나 산화제 등의 첨가로 반죽의 물성 개선에 관한 실험과 제빵 품질 특성에 대한 실험이 계속 필요하리라 생각된다.

• 한국동물위생학회지
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• 제40권2호
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• pp.119-131
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• 2017

Among different types of spoilage, microbial contamination can cause feed decomposition, which results in decreases in feed intake and productivity, infection, and breeding disorder. During the storage time, various microbes have a chance to inoculate with depreciation of feed and to infect the animals. We investigated bacteria that inhabit diverse feed ingredients and complete feed which have been stored for a few months. We isolated and identified 30 genera and 62 species of bacteria. Among these 62 species, 21 species were of non-pathogenic bacteria, 18 species were of pathogenic bacteria, 9 species were of opportunistic pathogens, and 14 species were of unknown bacteria. Pantoea allii and 24 species showed proteolytic enzyme activity. We also confirmed that 6 species including Pseudomonas psychrotolerans showed ${\alpha}$-amylase activity, and 29 species including Burkholderia vietnamiensis showed cellulase activity. Microbacterium testaceum and 3 species showed resistance to Ampicillin, Kanamycin, Streptomycin, Gentamicin, Carbenicillin, and Erythromycin ($50{\mu}g/mL$). Using mealworm larvae (Tenebrio molitor L.) as a model for pathogenicity, we confirmed that 8 species including Staphylococcus xylosus had pathogenicity for mealworm larvae. Especially, Enterobacter hormaechei, Staphylococcus xylosus, and Staphylococcus hominis were reported as being pathogenic for humans. This research suggests that hygienic management of animal feed is essential because beneficial and harmful bacteria can inhabit animal feed differently during storage and distribution.

• 한국식품조리과학회지
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• 제15권5호
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• pp.469-474
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• 1999

숭늉 제조용 누룽지 가루의 제조방법을 개발하기 의하여 쌀가루를 액화시키고 호화시킨 다음 hot plate에서 태우는 방법을 조사하였다. 누룽지 가루는 태움정도에 따라 3가지로 구분하였다 그 결과 액화를 더 할수록 쌀가루 분산액의 DE값이 증가하고 점도가 감소하였으며 이 현상은 액화 2분내에 현저하였다. 누룽지 가루로 숭늉을 80~10$0^{\circ}C$에서 추출하였을때 처음 10분간에 80% 이상의 총 고형분이 추출되었으며 추출온도가 높을수록 추출수율이 높았다. 숭늉의 침전된 고형분의 부피와 탁도는 추출온도와 시간이 길수록 높은 값을 보여주었다. 3가지 누룽지 가루 중 가장 많이 태운 것이 숭늉의 고형분 수율과 탁도가 가장 높고 L값이 가장 낮았다. 또한 향미와 색에 대한 관능검사 결과는 가장 많이 태운 것이 가장 높은 점수를 보여 주었지만 중간정도 태운 누룽지 가루 숭늉의 기호도가 가장 높은 결과를 보여주었다.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.251-261
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• 2017

Initially, we screened 18 Aspergillus sojae-like strains from Aspergillus spp. isolated from meju (Korean traditional fermented soybean brick) according to their morphological characteristics. Because members of Aspergillus section Flavi are often incorrectly identified because of their phylogenetic similarity, we re-identified these strains at the morphological and molecular genetic levels. Fourteen strains were finally identified as A. sojae. The isolates produced protease and ${\alpha}-amylase$ with ranges of 2.66-10.64 and 21.53-106.73 unit/g-initial dry substrate (U/g-IDS), respectively, which were equivalent to those of the koji (starter mold) strains employed to produce Japanese soy sauce. Among the isolates and Japanese koji strains, strains SMF 127 and SMF 131 had the highest leucine aminopeptidase (LAP) activities at 6.00 and 6.06 U/g-IDS, respectively. LAP plays an important role in flavor development because of the production of low-molecular-weight peptides that affect the taste and decrease bitterness. SMF 127 and SMF 131 appeared to be non-aflatoxigenic because of a termination point mutation in aflR and the lack of the polyketide synthase gene found in other A. sojae strains. In addition, SMF 127 and SMF 131 were not cyclopiazonic acid (CPA) producers because of the deletion of maoA, dmaT, and pks/nrps, which are involved in CPA biosynthesis. Therefore, A. sojae strains such as SMF 127 and SMF 131, which have high protease and LAP activities and are free of safety issues, can be considered good starters for soybean fermentations, such as in the production of the Korean fermented soybean products meju, doenjang, and ganjang.