• Title/Summary/Keyword: ${\alpha} -C_2S$

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컬럼 크로마토그라피에 의한 아스퍼질러스 계통의$\alpha$-아미라제 및 프로테아제의 結晶化(제 3 보) -Aspergillus oryzae S.H.W. 131의 $\alpha$-amylase의 結晶化 및 化學的 性質- (Crystallization of $\alpha$-amylase and protease of Asp. oryzae from Column Chormatography(III) - Crystallization and Chemical Properties of $\alpha$-Amylase of Aspergillus oryzae S.H.W. 131-)

  • 서항원
    • 미생물학회지
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    • 제10권3호
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    • pp.105-105
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    • 1972
  • The enzyme was produced by Asp.oryzae SHW 131. the enzymatic properties of .alpha.-amylase are following : 1) Crystallization of .alpha.-amylase is formed of longish square. 2) The range of stable pH is 5-10 and optimum ph is 5.5. 3) It is very unstable enzyme about EDTA and protection by $Ca^{++}$ ion and best activated at $50^{\circ}C$ about temperature. 4) Asp.oryzae SHW 131 produced .alpha.-amylase with acid-protease, neutral-protease and tepid-alkalin-protease.

Low operating voltage and long lifetime organic light-emitting diodes with vanadium oxide $(V_2O_5)$ doped hole transport layer

  • Yun, J.Y.;Noh, S.U.;Shin, Y.C.;Baek, H.I.;Lee, C.H.
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
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    • pp.1038-1041
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    • 2006
  • We report low operating voltage and long lifetime organic light-emitting diodes (OLEDs) with a vanadium oxide $(V_2O_5)-doped$ N,N'-di(1-naphthyl)- N,N'-diphenylbenzidine $({\alpha}-NPD)$ layer between indium tin oxide and ${\alpha}-NPD$. At a luminance of $1000\;cd/m^2$, $V_2O_5$ doped ${\alpha}-NPD$ device shows a operation voltage of 5.1V, while the device without $V_2O_5$ shows 5.8V. The $V_2O_5$ doped $({\alpha}-NPD)$ device also shows a longer lifetime and smaller operation voltage variation over time. It is suggested that the improved device performance can be attributed to the higher hole-injection efficiency and stability of the $V_2O_5$ doped $({\alpha}-NPD)$ layer.

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Xylanase 생산균 Streptomyces sp. WL-2의 특성과 효소 생산성 (Characterization and Xylanse Productivity of Streptomyces sp. WL-2)

  • 이은희;김창진;윤기홍
    • 한국미생물·생명공학회지
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    • 제33권3호
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    • pp.178-183
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    • 2005
  • 토양으로부터 세포외로 xylanase를 분비 생산하는 방선균 WL-2가 분리되었으며, 분리균의 16S rRNA 염기서열과 형태${\cdot}$배양${\cdot}$생리적 특성을 조사한 결과 Streptomyces속 균주로 확인되었다. 분리균의 배양상등액에 존재하는 xylanase는 pH 6.0과 $60^{\circ}C$의 반응조건에서 반응성이 가장 높았으며, pH $4.5{\~}6.5$ 범위에서 최대활성의 $90{\%}$ 이상을 나타냈다. Xylanase의 생산을 위한 배지를 최적화하기 위해서 G.S.S 배지성분을 여러 종류의 탄수화물로 대체하였다. ${\alpha}-Cellulose$, oat spelt xylan과 엿당과 같은 탄수화물은 Streptomyces sp. WL-2의 xylanase 생산성을 급격히 증가시키는 것으로 확인되었다. ${\alpha}-Cellulose(1\%)$와 엿당($1{\%}$)을 함유한 변형배지에서 xylanase의 최대생산성이 120 U/ml로 확인되었다.

연제품류의 열확산도 추정에 관한 연구 5. 적색육 어육 연제품의 열확산도 (Prediction of Thermal Diffusivfties of Fish Meat Paste Products 5. Thermal Diffusivities of Red Muscled Fish Meat Paste Products)

  • 한봉호;최수일;김종철;배태진;조현덕
    • 한국수산과학회지
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    • 제21권6호
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    • pp.366-370
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    • 1988
  • In Previous paper, an experimental equation for prediction of thermal diffusivity of white muscled fish meat paste products was suggested. In this paper, another experimental equation for red muscled fish meat paste products was derived. The thermal diffusivities of products with water contents of 44.74 to $82.83\%$ and lipid contents of 0.35 to $23.38\%$ could be deduced as following equations ; $$\alpha_{80.39^{\circ}C}=0.0759{\cdot}10^{-6}{\cdot}X_w+0.0836{\cdot}10^{-6},\;m^2{\cdot}s^{-1}$$ $$\alpha_{100.63^{\circ}C}=0.0820{\cdot}10^{-6}{\cdot}X_w+0.0853{\cdot}10^{-6},\;m^2{\cdot}s^{-1}$$ $$\alpha_{120.09^{\circ}C}=0.0830{\cdot}10^{-6}{\cdot}X_w+0.09140{\cdot}10^{-6},\;m^2{\cdot}s^{-1}$$ The experimental equation derived from these equations was ; $$\alpha=(0.9795+.8996{\cdot}X_w){\cdot}\alpha_w-0.0709{\cdot}10^{-6}{\cdot}X_w-0.0779{\cdot}10^{-6},\;m^2{\cdot}s^{-1}$$ The errors of the thermal diffusivities of red muscled fish meat paste products predicted with this equation were less than ${\pm}\;0.52\%$ compared with those measured. A new experimental equation for products of white and red muscled fish meat paste could be discribed as follow ; $$\alpha=(1.096+0.5318{\cdot}X_w){\cdot}\alpha_w-0.0057{\cdot}10^{-6}{\cdot}X_w-0.0992{\cdot}10^{-6},\;m^2{\cdot}s^{-1}$$ The errors of the thermal diffusivities predicted with this equation were less than ${\pm}\;0.13\%$ compared with those measured.

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개에서 $\alpha$-Toxin을 생성하는 Type A Clostridium perfringens에 의한 장염 (Enteritis Caused by Type A Clostridium perfringens Producing $\alpha$-Toxin in a Dog)

  • 한재익;장혜진;천행복;김지혜;김근형;장동우;나기정
    • 한국임상수의학회지
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    • 제27권2호
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    • pp.190-193
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    • 2010
  • 6년령, 암컷 시베리안 허스키가 점액성 설사로 충북대학교 동물의료센터에 내원하였다. 분변 검사 결과 심한 장상피세포 박리와 함께 다수(>70%)의 아포형성 간균 증식이 관찰되었다. 분자생물학적 세균 동정 결과 증식된 세균은 Clostridium perfringens로 확인되었으며, toxin 검사 결과 $\alpha$-toxin이 세균에 의해 합성되고 있음을 확인하였다. 따라서 환자는 $\alpha$-toxin을 합성하는 C. perfingens에 의한 장염으로 진단하였으며, amoxicillin/clavulanate를 투여하였다. 치료 1주 후 설사는 소실되었으며, 분변 검사 결과 아포 형성 간균은 소실되었다. 이 증례는 빠르고 정확한 진단으로 type A C. perfringens에 의한 장염이 효과적으로 치료됨을 보여준다.

${\alpha}$-셀룰로오즈의 열분해에 관한 연구(I) - 산촉매 NaCl의 영향 - (Kinetics of Pyrolysis Degradation of on ${\alpha}-Cellulose$. - Effect of Acid Catalysts NaCl-)

  • 나상도;황준호;최경선;설수덕;손진언
    • Elastomers and Composites
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    • 제31권2호
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    • pp.122-129
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    • 1996
  • The Thermal decomposition of the ${\alpha}-Cellulose$ and NaCl was studied using a thermal analysis technique in the steam of nitrogen gas with 30ml/min at various heating ranges from 4 to $20^{\circ}C/min$. The Derivative and Integral method used to be obtained values of activation energy of decomposition reaction. 1. The values of activation energy evaluated by Derivative and Intergral method were consistent with each other very well. 2. The maximum value of heat of decomposition evalated by DSC method was ${\alpha}-Cellulose/NaCl= 90/10$. 3. The thermogravimetric trace curve agreed with the theoretical equation.

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Molecular Theory of Plastic Deformation (Ⅲ)$^*$

  • Kim, Jae-Hyun;Ree, Tai-Kyue;Kim, Chang-Hong
    • Bulletin of the Korean Chemical Society
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    • 제2권3호
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    • pp.96-104
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    • 1981
  • (1) The flow data of f (stress) and ${\dot{s}$ (strain rate) for Fe and Ti alloys were plotted in the form of f vs. -ln ${\dot{s}$ by using the literature values. (2) The plot showed two distinct patterns A and B; Pattern A is a straight line with a negative slope, and Pattern B is a curve of concave upward. (3) According to Kim and Ree's generalized theory of plastic deformation, pattern A & B belong to Case 1 and 2, respectively; in Case 1, only one kind of flow units acts in the deformation, and in Case 2, two kinds flow units act, and stress is expressed by $f={X_1f_1}+{X_2f_2}$where $f_1\;and\;f_2$ are the stresses acting on the flow units of kind 1 and 2, respectively, and $X_1,\;X_2$ are the fractions of the surface area occupied by the two kinds of flow units; $f_j=(1/{\alpha}_j) sinh^{-1}\;{\beta}_j{{\dot{s}}\;(j=1\;or\;2)$, where $1/{\alpha}_j\;and\;{\beta}_j$ are proportional to the shear modulus and relaxation time, respectively. (4) We found that grain-boundary flow units only act in the deformation of Fe and Ti alloys whereas dislocation flow units do not show any appreciable contribution. (5) The deformations of Fe and Ti alloys belong generally to pattern A (Case 1) and B (Case 2), respectively. (6) By applying the equations, f=$(1/{\alpha}_{g1}) sinh^-1({\beta}_{g1}{\dot{s}}$) and $f=(X_{g1}/{\alpha}_{g1})sinh^{-1}({\beta}_{g1}{\dot{s}})+ (X_{g2}/{\alpha}_{g2})\;shih^{-1}({\beta}_{g2}{\dot{s}})$ to the flow data of Fe and Ti alloys, the parametric values of $x_{gj}/{\alpha}_{gj}\;and\;{\beta}_{gs}(j=1\;or\;2)$ were determined, here the subscript g signifies a grain-boundary flow unit. (7) From the values of ($({\beta}_gj)^{-1}$) at different temperatures, the activation enthalpy ${\Delta}H_{gj}^{\neq}$ of deformation due to flow unit gj was determined, ($({\beta}_gj)^{-1}$) being proportional to , the jumping frequency (the rate constant) of flow unit gj. The ${\Delta}H_{gj}\;^{\neq}$ agreed very well with ${\Delta}H_{gj}\;^{\neq}$ (self-diff) of the element j whose diffusion in the sample is a critical step for the deformation as proposed by Kim-Ree's theory (Refer to Tables 3 and 4). (8) The fact, ${\Delta}H_{gj}\;^{\neq}={\Delta}H_{j}\;^{\neq}$ (self-diff), justifies the Kim-Ree theory and their method for determining activation enthalpies for deformation. (9) A linear relation between ${\beta}^{-1}$ and carbon content [C] in hot-rolled steel was observed, i.e., In ${\beta}^{-1}$ = -50.2 [C] - 40.3. This equation explains very well the experimental facts observed with regard to the deformation of hot-rolled steel..

Bis(N-Methylphenazinium) Bis(Oxalato)Palladate(Ⅱ)의 결정구조 (The Crystal Structure of Bis(N-Methylphenazinium) Bis(Oxalato)Palladate(Ⅱ))

  • 김세환;남궁해;이현미
    • 대한화학회지
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    • 제38권11호
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    • pp.827-832
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    • 1994
  • (N-methylphenazinium) bis(oxalato)palladate(II)$((C_{13}H_{11}N_2)_2[Pd(C_2O_4)_2])$의 착이온 및 결정의 구조는 X-선 회절법으로 연구하였다. 이 결정은 사방정계이고 공간군은 P1 (군번호 = 2)이다. 단위세포 길이는 a = 7.616(8), b = 9.842(3), c = $20.335(7)\AA$, $\alpha$ = 103.53(3), $\beta$ = 90.00(5), $\gamma$ = $112.38(5)^{\circ}이며$, $V = 1363(2){\AA}^3,\;F_w = 672.93,\;D_c = 1.639\;gcm^{-3},\;F(000) = 680.0,\;{\mu} = 7.3\;cm^{-1},\;Z = 2$이다. 회절반점들의 세기는 흑연 단색화 장치가 있는 자동 4축 회절기로 얻었으며 $Mo-K\alpha$ X - 선(${\lambda}$= 0.7107 $\AA)$을 사용하였다. 구조분석은 중금속법으로 풀었으며, 최소자승법으로 정밀화하였고, 최종 신뢰도 값들은 3120개의 회절반점에 대하여 $R = 0.069,\;R_w = 0.050,\;R_{all} = 0.069$ 및 S = 5.45였다. 착이온들은 근본적인 평면구조로써, 이들의 충진구조는 착음이온들을 두개의 양이온들이 거의 평행하게 둘러싸고 있는 삼중체들을 형성하고 있다. 양이온과 음이온들의 이면각들이 각각 6.3(6)과 $57.06(6)^{\circ}$인 삼중체들이 b축을 따라서 배열되어 있으나, 삼중체면의 배향은 두 가지 착음이온의 이면각이 $59.08(9)^{\circ}$을 이루는 방향이다. 삼중체내의 면간거리는 각각 3.328와 3.463 $\AA$이었다.

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사철나무의 알칼로이드 성분 (Sesquiterpene Pyridine Alkaloids from Euonymus japonica)

  • 류재하;은진희;이소영;장준식;박만기;박정일;한용남;한병훈
    • 약학회지
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    • 제41권5호
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    • pp.554-558
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    • 1997
  • Two alkaloidal components were isolated from the ether soluble part of the MeOH extract of the root bark of Euonymus japonica. Their structures were elucidated by the spe ctroscopic analylses as the sesquiterpene pyridine alkaloids derived from polyester sesquiterpenes which are characteristically detected in Celastraceae plants. These include macrocycle formed by two ester linkages between dihydro-${\beta}$-agarofuran nucleus and pyridinic dibasic acid(compound 1:evoninic acid, compound 2:wilfordic acid). The structure of compound $1[C_{47}H_{50}N_2O_{17},\;mp\;161{\sim}163^{circ}C$. $[{\alpha}]_D^{28}=+31.6^{\circ}$(c, 0.1 in EtOH)] was determined as novel structure named as euojaponine N, and compound $2[C_{48}H_{57}NO_{18},\;mp\;142{\sim}145^{circ}C,\;[{\alpha}_D^{27}=+27.0^{\circ}$(c, 0.1 in EtOH)] was identified as ebenifoline W-I reported from Maytenus ebenifolia.

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Dexamethasone Facilitates NF-κB Signal Pathway in TNF-α Stimulated Rotator Cuff Tenocytes

  • Ji, Jong-Hun;Kim, Young-Yul;Patel, Kaushal;Cho, Namjoon;Park, Sang-Eun;Ko, Myung-Sup;Park, Suk-Jae;Kim, Jong Ok
    • Journal of Microbiology and Biotechnology
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    • 제29권2호
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    • pp.297-303
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    • 2019
  • Corticosteroids are commonly used for pain control in rotator cuff tear. Deregulated $NF-{\kappa}B$ activation is a hallmark of chronic inflammatory diseases and has been responsible for the pathogenesis of rotator cuff tear. The Dexamethasone(DEXA) is a synthetic corticosteroid. The purpose of this study was to examine the exact effect of dexamethasone on $NF-{\kappa}B$ signaling in rotator cuff tear. We measured $NF-{\kappa}B$ expression in four groups: control, $TNF-{\alpha}$-treated, DEXA-treated, and combined treatment with $TNF-{\alpha}$ and DEXA. Tenocytes were isolated from patients with rotator cuff tears and pre-incubated with $TNF-{\alpha}$ (10 ng/ml), DEXA ($1{\mu}M$), or both of them for 10 min, 1 h, and 2 h. Expression of p65, p50, and p52 in the nuclei and cytosol was analyzed by western blotting and immunofluorescence imaging using confocal microscopy. We also evaluated nucleus/cytosol (N/C) ratios of p65, p50, and p52. In our study, the combined treatment with DEXA and $TNF-{\alpha}$ showed increased N/C ratios of p65, p50, and p52 compared with those in the $TNF-{\alpha}$ group at all time points. Additionally, in the DEXA group, N/C ratios of p65, p50, and p52 gradually increased from 10 min to 2 h. In conclusion, DEXA promoted the nuclear localization of p65, p50, and p52, but was not effective in inhibiting the inflammatory response of $TNF-{\alpha}$-stimulated rotator cuff tear.