• Korean Journal of Food Science and Technology
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• v.21 no.4
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• pp.583-589
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• 1989

In order to improve the quality and yields of barley tea(water extracts), enzymatic hydrolysis was carried out with using three kinds of mired enzymes of ${\alpha}-amylase,\;{\beta}-amylase$ and protease. The barley treated with enzymes were the crushed , soaked and slightly roasted(light brown) Youngsanbori(hulless) and the physical, chemical and sensory characteristics were investigated. The results showed that enzymatic hydrolysis caused a signigicant increase in solid yields (80%) of barley tea, particularly with the mixed enzyme 5,000 which has higher activity of ${\alpha}-amylase$ than the other enzymes. The intrinsic viscosity of barley tea was reduced by enzymatic hydrolysis as the value of dextrose equivalent increased The Hunter L, a, b values was lower for the enzyme treated barley tea but showed not significant difference with reaction time. The organoleptic evaluation clearly showed that both intensity and acceptability of odor and taste was markedly Increased. The roasted nutty, and sweety odor and taste were particular in increase by mixed enzyme 5,000.

• The Korean Journal of Pharmacology
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• v.25 no.1
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• pp.1-11
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• 1989

In this study, it was aimed to investigate the role of serotonergic neurotransmission in nucleus tractus solitarius (NTS) for the central regulation of blood pressure and heart rate and its involvement in baroreceptor reflex activation in rats. A microinjection of 5-hydroxytryptamine (5-HT) into the NTS produced decreases in blood pressure and heart rate. Maximal decreases were $34.4{\pm}1.6$ mmHg and $41.7{\pm}10.2$ beats per min by 300 pmol of 5-HT. Microinjections of ${\alpha}-methylnor-adrenaline$ $({\alpha}-MNE)$ and clonidine manifested similar decreases in blood pressure and heart rate. The hypotensive and bradycardial effects of 5-HT were blocked by previous applications of 5-HT antagonists, ritanserin, methysergide and ketanserin into the NTS, respectively. By pretreatment with reserpine and 6-hydroxydopamine (6-OHDA, i.c.v.), both hypotensive and bradycardial effects of 5-HT were significantly attenuated. Pretreatment with 5, 7-dihydroxytryptamine (5,7-DHT, i.c.v.) enhanced the hypotensive and bradycardial effects of 5-HT. Similarly, following pretreatment with 6-OHDA, the effects of clonidine were increased. Pretreatment either with 5,7-DHT or 6-OHDA significantly attenuated the sensitivity of baroreflex produced either by phenylephrine or by sodium nitroprusside. When either 5,7-DHT or 6-OHDA was injected into the NTS $(5,7-DHT;\;8{\mu}g\;6-OHDA;\;10{\mu}g)$, both of the baroreflex sensitivities were impaired. In the immunohistochemical study, the injection of 6-OHDA into the the NTS led to reduction of axon terminal varicosity, however, the injection did not reduce the numbers of catecholaminergic cell bodies. Likewise, when 5,7-DHT was injected into the NTS, the varicosity of serotonergic axon terminals was markedly reduced. Based on these results, it is suggested that (1) stimulation of serotonergic receptors in the NTS leads to decreases in blood pressure and heart rate as observed with the stimulation of catecholaminergic system, (2) both serotonergic and catecholaminergic receptors may be located postsynaptically, and (3) the serotonergic neurons as well as catecholaminergic neurons may have a close relevance for the activation of baroreflex.

• Microbiology and Biotechnology Letters
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• v.45 no.2
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• pp.162-167
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• 2017

For the production of bioethanol by the synchronous saccharification and fermentation (SSF) process, bio-capsule formation was attempted. Many saccharifying fungal strains and fermentative yeast strains were first screened. Aspergillus sp. BCNU 6200, Penicillium sp. BCNU 6201, and P. chrysogenum KACC 44363 were found to be excellent producers of saccharifying enzymes such as ${\alpha}$-amylase and glucoamylase. Saccharomyces cerevisiae IFO-M-07 showed the highest ethanol productivity among the tested strains. Secondly, we determined the optimal conditions for pellet formation, and those for bio-capsule formation. All the tested fungal strains formed pellets, and the optimal conditions for bio-capsule formation were $28^{\circ}C$ and 120 rpm. Lastly, SSF process was performed using a bio-capsule. An ethanol yield of 3.9% was achieved by using the Aspergillus sp. BCNU 6200 bio-capsule (Aspergillus sp. BCNU 6200 + S. cerevisiae IFO-M-07) at $30^{\circ}C$ with shaking at 120 rpm during the 10 days of incubation. The results provide useful information on the application of a bio-capsule in bioethanol production under the SSF process.

• 한국가스학회:학술대회논문집
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• 1997.09a
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• pp.121-126
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• 1997

우리나라는 그동안 가스산업에서 고속성장을 이룩해 왔으나, 근래에는 중대가스산업사고가 빈번하게 일어나고 있다. 그런 이유로 1996년도에는 중대산업사고 예방제도가 전면적으로 실시되고 공정안전보고서를 자성하여 제출하도록 함으로서 위험성 평가의 중요성이 점차 높아가고 있으며, 위험성 평가 기법들에 대하여 여러 연구 단체에서 연구${\cdot}$ 시도되고 있다. 또한 이러한 연구${\cdot}$보고된 위험성 평가 기법들을 프로그램화하고 적용하려는 시도가 많이 있어 왔다. 본 연구에서는 기존에 연구${\cdot}$개발된 위험성 평가 기법들에 최근 컴퓨터 산업의 발달에 힘입어 각광을 받고 있는 지리정보시스템을 적용하고자 한다. 이러한 가스산업시설의 위험성 평가 시스템은 위험성을 평가하기 위해서 필요한 여러 가지 정보들을 지리정보시스템이 속성 데이터로서 저장하고 있으며, 가스산업시설에 관련된 주변의 도민들을 공간 데이터로서 저장하고 있다. 그리고 위험성 평가 시스템의 세부적인 기능을 모듈화하였다. 우선 위에 언급한 속성 데이터와 공간 데이터를 관리하는 모듈과 이러한 데이터를 가지고 사고영향 범위를 산출해내기 위한 모듈, 그리고 산출된 사고 영향 범위를 도면에 나타내는 모듈로 나뉘어져 있다. 이렇게 지리정보시스템에 구축되어 있는 도면에 위험성을 평가한 결과치를 나타냄으로서 위험성 평가 숙련자가 아니더라도 위험성 평가를 할 수 있고 결과를 분석하도록 도와 줄 수 있도록 할 수 있다. 또한 향후 재난관리시스템에서는 도면상의 도로에 교통량 가중치와 인근 소방서와 경찰서등의 위치를 관리하도록 지리정보시스템을 적용할 수 있으며, 가스시설물 관리시스템에서는 최근 대형가스사고의 대부분이 타공사에 의한 것임을 고려하여 가스배관망을 포함하여 기타 다른 지하배관망을 관리하도록 지리정보시스템을 적용할 수 있다.기질로서 이용하지 못하므로 invertase 및 ${\alpha}-glucosidase$ 활성이 없는 것으로 나타났다.)의 모양은 배양에 의해 형성된 균핵(菌核)의 모양과 다소 차이가 있었다. R. cerealis의 균사생장(菌絲生長) 적온(適溫)은 $23^{\circ}C$였으며, 다른 균핵진균(菌核眞菌)들의 균사생장(菌絲生長) 적온(適溫)은 $27-33^{\circ}C$였다. 일부 균핵진균(菌核眞菌)들의 균사생장(菌絲生長) 최고온도(最高溫度)는 $41^{\circ}C$ 로서 높은 반면, R. cerealis 의 균사생장(菌絲生長) 최고온도(最高溫度)는 $31^{\circ}C$로서 낮았다. R. cerealis 의 균사생장(菌絲生長) 최저온도(最低溫度)는 $2^{\circ}C$ 였으며, 다른 균핵진균(菌核眞菌)들의 균사생장(菌絲生長) 최저온도(最低溫度)는 $6-10^{\circ}C$였다. 시술(施術)하였다. 10. 한양방향시치료시(韓洋方同時治療時) 양호이상(良好以上)의 효과(效果)를 보인 경우(境遇)가 30건중(件中) 21건(件)으로 70%의 치료효과(治療效果)를 보여 한방단일진료시(韓方單一診療時)보다 다소 높은 치료효과(治療效果)를 나타냈다. 11. 1일평균(日平均) 한방진료건수(韓方診療件數)는 26.95명(名)이었고, 1일평균(日平均) 한방초진환자수(韓方初診患者數)는 3.9명(名)으로 나타났으며, 한방진료(韓方診療)가 양한방(洋韓方) 총진료(總診療)의 23.20%를 차지했고, 1일(日) 평균(平均) 한양방(韓洋方) 총진료건수(總診療件數)에 대(對)해 1일(日) 평균(平均) 한방초진건수(韓方初診件數)가 21.51%를 차지하는 것으로 나타났다.험이 완료된 31명에서는 부작용이 없거나 경도의 부작용이 있었으나 임상적

• Korean Journal of Food Science and Technology
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• v.31 no.3
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• pp.619-624
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• 1999

This work was to study the potential health food use of germinated colored rice after germinating and drying using microwave under vacuum. Colored rice was soaked in water at $15^{\circ}C$ for 2 days and then germinated at $25^{\circ}C$ for $3{\sim}4\;days$. The germinated colored rice was dried by different drying methods: microwave vacuum drying 1, microwave vacuum drying $2\;(drying{\rightarrow}crushing{\rightarrow}drying)$, hot air drying, vacuum drying and freeze drying. Each drier except freeze drier was set to maintain the sample temperature at $60^{\circ}C$. During microwave vacuum drying 1 or 2, the sample reached $60^{\circ}C$ much faster (within 5 min) and was dried much faster ($2{\sim}3\;hrs$ than the other drying methods. The initial drying rate of microwave vacuum drying was ten times faster than that of hot air drying. The microwave vacuum drying 2 retained the highest ${\alpha}-amylase$ activity, followed by microwave vacuum drying 1, freeze drying, vacuum drying, and hot air drying.

• Sleep Medicine and Psychophysiology
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• v.8 no.2
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• pp.121-128
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• 2001

Objectives: In narcoleptic patients diagnosed with ICSD (international classification of sleep disorders, 1990) criteria, nocturnal polysomnography, and MSLT (multiple sleep latency test), we tried to find characteristic features of quantitative electroencephalography (QEEG) in a wakeful state. Methods: We compared eight drug-free narcoleptic patients with sex- and age-matched normal controls, using computerized electroencephalographic mapping technique and spectral analysis. Absolute power, relative power, interhemispheric asymmetry, interhemispheric and intrahemispheric coherence, and mean frequency in each frequency band (delta, theta, alpha and beta) were measured and analyzed. Results: Compared with normal controls, narcoleptic patients showed decrease in monopolar interhemispheric coherence of alpha frequency bands in occipital ($O_1/O_2$), parietal ($P_3/P_4$), and temporal ($T_5/T_6$) areas and beta frequency band in the occipital ($O_1/O_2$) area. Monopolar intrahemispheric coherences of alpha frequency bands in left hemispheric areas ($T_3/T_5$, $C_3/P_3$ & $F_3/O_1$) decreased. Decrease of monopolar interhemispheric asymmetry of delta frequency band in the occipital ($O_1/O_2$) area was also noted. The monopolar absolute powers of beta frequency bands decreased in occipital ($O_2,\;O_z$) areas. Conclusion: Decreases in coherences of narcoleptic patients compared with normal controls may indicate fewer posterior neocortical interhemispheric neuronal connections, and fewer left intrahemispheric neuronal connections than normal controls in a wakeful state. Therefore, we suggest that abnormal neurophysiological sites of narcolepsy may involve complex areas such as neocortex and subcortex as well as the brainstem.

• Korean Journal of Microbiology
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• v.46 no.2
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• pp.192-199
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• 2010

The alone and combined effects of bacteriocin produced from Enterococcus faecalis MJ-213 and potassium sorbate against the food-borne pathogenic bacteria were studied. Bacteriocin minimal inhibitory concentration (MIC) values for Staphylococcus aureus ATCC 6538 and Salmonella enteritidis ATCC 13076 were 50 and 100 ${\mu}g$/ml, respectively. Bacteriocin (100 ${\mu}g$/ml) alone was active against S. aureus and S. enteritidis, but it was lower in antimicrobial effectiveness than the combination of bacteriocin (100 ${\mu}g$/ml) with potassium sorbate (100 ${\mu}g$/ml), which reduced initial counts (6 log cycle) of S. aureus and S. enteritidis by 1 and 3 log cycle, respectively. The bactericidal activity of bacteriocin of E. faecalis MJ-213 heated at $100^{\circ}C$ for 30 min or $121^{\circ}C$ for 15 min was markedly decreased as compared with the control. Moreover, the activity of bacteriocin was completely abolished by pepsin or protease II, but not affected by ${\alpha}$-amylase or lipase. The activity of bacteriocin adjusted to pH 6.0-8.0 showed almost the same inhibition ratio compared with the bacteriocin unadjusted pH, and though the inhibition ratio against pathogenic bacteria was reduced than the control, the bacteriocin was stable at pH 4.0 or 10.0, relatively. Furthermore, the combined treatment of bacteriocin and potassium sorbate than the alone treatment of bacteriocin significantly decreased (p<0.05) the viable cell counts of S. aureus or S. enteritidis inoculated on grind beef during storage at $4^{\circ}C$.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.6
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• pp.903-911
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• 2011

In this study, Bacillus polyfermenticus CJ6 harboring antibacterial activity was isolated from meju. The antibacterial activity of Bacillus polyfermenticus CJ6 was stable in the pH range of 3.0~9.0, but it disappeared after culture at $70^{\circ}C$ for 24 hr. Antibacterial activity was inactivated by proteinase K, protease, and ${\alpha}$-chymotrypsin, indicating its proteinaceous nature. The growth inhibitory effects of B. polyfermenticus CJ6 culture on food-borne pathogens such as Staphylococcus aureus, Salmonella Typhi, Listeria monocytogenes, and Escherichia coli O157:H7 were examined in this study. Approximately 6~6.2 log CFU/mL of each pathogen was co-cultured with B. polyfermenticus CJ6 in a 50 mL culture volume for 24 hr. Growth of S. aureus and L. monocytogenes was completely inhibited after 3 hr of incubation. Growth of S. Typhi and E. coli O157:H7 was also completely inhibited after 6 hr of incubation. The antibacterial compounds from B. polyfermenticus CJ6 were purified by solid phase extraction (C18 Sep-pak cartridge), recycling preparative HPLC, and analytical HPLC. Ultra-high performance liquid chromatography and electrospray ionization tandem mass spectrometry analysis were used to identify the purified antibacterial compounds, which were confirmed to be five peptides (757.4153 Da, 750.3444 Da, 1024.5282 Da, 1123.6083 Da, and 1617.8170 Da).

• Proceedings of the Korean Vacuum Society Conference
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• 1998.02a
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• pp.24-24
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• 1998

반도체장비를 포함하는 초고진공장비의 園훌化가 급속히 그리고 절실히 요구되고 있는 것이 현실정이다. 當面해서 실현할 국산진공장비의 대상은 廣範圍하다. 즉, 각종 진공 pump ( (rotary, dry, diffusion, cryo, ion, turbo melecular pump), 진공 chamber, 진공 line, gate valve 를 위 시 한 진공 V머ve, flange, gasket, fl않d야lU, mainpulater 퉁 진공 部品이 다. 진공계 의 핵심 은 適切하고 優良한 진공재료의 선태파 사용이다. 진공장비는 사용자가 원하는 진공도를 원하 는 시간 동안 륨空度를 유지해 주어야 한다. 진공재료 선태의 기준사항은:(1) 기체의 透過성 (2) 薰했훌 (3) 혔體放出특성 - -outgassing과 degassing- (4) 機械的 량훌度 (5) 온도 의존성 (6) 化學톡성 (7) 加I성 및 鎔接 성 (8) 課電특성 (9) 磁氣특성 (10) 高速함子 및 放射線 특성 (11) 經濟성 및 調達생 둥이 다. 우량한 초고진공계재료는 풍부하게 개발되어 왔고, 또 新材料들이 개발되고 있다. 여기에서는 주로 초고진공 내지는 극고진공계의 構造材料, 機能材料, 部品材料 일반파 몇가지 신재료의 특 성에 관해서 記述한다. M Mild SteeHSAE, 1112, 1010, 1020, 1022, etc)., S Stainless SteeHAlSI, 304, 304L, 310, 316, 321, 347): 구조재료, chamber, fl하1ges A Aluminum과 Alloys (1060, 1100, 2014, 4032, 6(뻐1): 구조재료, chamber, flanges, gaskets A AI, Al 떠loy는 SS에 代替하는 역 할올 시 작하고 있다. C Copper, Copper Alloys(C11$\alpha$)0, C26800, C61400, Cl7200): 내장인자, gasket, cryopanel, tubing T Titanium, Ziriconium, Haf띠um 및 Alloys: 특히 Ti은 10n pump 용 getter material 이 외 에 U UHV,XHV용 chamber계로서 관심올 끌고 있다. N Nickel, Nickel Alloys (200, 204, 211, monel, nichrome): 부식 방지 , 전자장치 , 자기 장치 귀 금속(Ag, Au, Pt, Pd, Rh, Ir, Os, Ru): 보조부품, gasket, filament, coating, thermocouple, 접 합부위 T TiC, SiC, zrC, HfC, TaC 둥의 탄화물과, BN, TiN, AlN 동의 질화물, 붕화물이 둥장하고 었 다. 유리: Soda Lime, Borosilicate, Potash Soda Lead: View Port, Chamber envelope C Ceramics: AlZ03, BeO, MgO, zrOz, SiOz, MgOzSiOz, 3Alz032SiOz, Z$textsc{k}$hSiOz S상N4: e electrical, thermal insulators, crucibles, boats, single crystals, sepctr려 windows 저자는 최근 저자들이 발견한 Zr-Ti-Cu-Ni-Be amorphous alloys coated cham뾰r가 radiation p proof로 이용될 수 있는 사실을 점검하고 었다 .. Z.Y. Hua 들은 Cs3Sb를 새로운 photocathode 재료로 보고하고 있다.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.141-142
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• 2003

본 연구는 전복의 유생사육을 목적으로 먹이생물인 부착미세조류의 파판 배양시 배양기간에 따른 종조성과 영양성분의 변화를 파악하기 위하여 수행되었다. 실험은 완도지역에서 봄철인 2~4월에 8주 동안 실시되었다. 배양을 위한 기초 조사로 배양지역 연안해수의 수온, 염분, pH, 총질소, 총인, Chlorophyll-a를 측정 하였다. 실험에 사용된 파판은 전복 종묘생산에 이용되는 규조배양용 파판과 틀을 이용하여 2주마다 파판을 수거한 뒤 부착된 미세조류를 부드러운 솔로 분리하여 원심분리한 후 지방산과 아미노산을 측정하였다. 각각의 시료는 광학현미경(Nikon)으로 동정한 후 Sedwick-rafter counting chamber를 이용하여 종별로 계수하고 이를 단위면적당 개체수 (cells/$\textrm{cm}^2$)로 환산하여 정량분석을 하였다. 실험 결과 봄철 완도지역의 평균 수온과 염분은 각각 15.4$^{\circ}C$와 33.88$\textperthousand$이었고 pH는 8.21이었다. 총질소와 총인은 각각 0.078mg/$\ell$, 0.018mg/$\ell$였고 chlorophyll-$\alpha$는 1.47$\mu$g/$\ell$ 였다. 부착미세조류의 아미노산 분석 결과는 배양 2주째의 총아미노산 양이 5.63%로 가장 낮았고, 4주는 6.50%, 6주 7.82% 8주 7.87%로, 6주까지 증가하다가 정체하는 경향을 보였다. 필수아미노산의 경우 총아미노산의 경향과 비슷하였고 2, 4, 6, 8주째의 양은 각각 2.75%, 3.16%, 4.01%, 3.96%였다. 필수 아미노산과 비필수아미노산의 비는 2, 4, 6, 8주 각각 0.95, .095, 1.05, 1.01의 결과를 보였다. 지방산의 경우 palmitic acid( $C_{16:0}$)와 palmitoleic acid( $C_{16:1}$)가 43.10~67.66%의 함량을 보였으나 4주째에는 검출되지 않았으며 EPA는 6주에서 10.97%를 보였고 나머지 구간에서는 검출되지 않았다. 부착미세조류의 biomass는 기간별로 2.4$\times$$10^3$ cells/$\textrm{cm}^2$, 7.8$\times$$10^3$ cells/$\textrm{cm}^2$ 25.1$\times$$10^3$ cells/$\textrm{cm}^2$, 44.3$\times$$10^3$ cells/$\textrm{cm}^2$ 이었다. 출현량은 총 64종으로 대부분이 부착규조류였으며 2주, 4주, 6주, 8주 각각 43종, 25종, 19종, 24종의 부착 규조류가 출현하였다. 그리고 우점종은 2주째 Nitzschia petitiana, Fragilia psedonana, 4주 Navicula mollis, Flagilia cylindrus, 6주 Flagilia capucaina, Achnanthes haukiana, 8주 Flagilia islandica, Flagilia cylindrus였다.s였다.