• Title/Summary/Keyword: ${\alpha}$-Method

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Production of Mozzarella Cheese Analogue by Ultrafiltration (한외여과를 이용한 Mozzarella Cheese Analogue 제조에 관한 연구)

  • Lee, Sung-Hui;Song, Kwang-Young;Seo, Kun-Ho;Yoon, Yoh-Chang
    • Journal of Dairy Science and Biotechnology
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    • v.31 no.1
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    • pp.21-33
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    • 2013
  • The purpose of this study was to improve the production of Mozzarella cheese analogues manufactured using mixtures of soy milk and concentrated raw milk by performing ultrafiltration (UF) and to assess the quality of these cheeses during a 30-day storage period at $4^{\circ}C$, relative to that of Mozzarella cheese manufactured with the traditional method. The solid consistency of Mozzarella cheese analogue prepared from milk mixtures was lower than that of cheese manufactured from raw milk or soy milk and increased during storage, which is considered to be the result of decreasing water levels, as well as with increasing soy milk concentrations. In the Mozzarella cheese analogue generated using the milk mixtures, the fat content decreased with increase in the soy milk concentration, while it decreased during the storage period. Lactose levels were lowest in cheese composed of soy milk or raw milk and processed by UF, and decreased during storage in cheese produced using milk mixtures. In milk mixtures containing soy milk, the protein concentration increased with increasing amounts of raw milk and did not change during the storage period. The water-soluble nitrogen compound level was similar between cheeses and increased only slightly during storage. The amount of non-protein nitrogen compounds was higher in the cheese analogue than in the control cheese and tended to increase during storage. Analysis of the physicochemical traits of the Mozzarella cheese analogue yielded the following results: During storage, titratable acidity levels increased while pH tended to decrease. After analysis using electropherograms, it was classified as ${\alpha}$-, ${\beta}$-, or ${\kappa}$-casein. The results of rheometry tests showed that in the Mozzarella cheese analogue prepared from milk mixtures, with raw milk concentrated by UF, increases in concentration rate lead to lowered hardness, elasticity, cohesiveness, and brittleness. When cheese was produced from milk mixtures and concentrated by UF, meltability increased as the concentration rate increased, although to an extent that was less than that observed for the control cheese, and tended to increase during storage. Sensory evaluation showed that the analogue cheese was much better than the control cheese in terms of formation, appearance, and flavor.

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Reliability Analysis of Emotion Evaluation EPA.PAD Model in Each Design Field (디자인 분야별 EPA.PAD 감성평가모형의 신뢰도 분석)

  • Kim, Ji-Hye;Lee, Jin-Sook
    • Science of Emotion and Sensibility
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    • v.17 no.1
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    • pp.79-92
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    • 2014
  • This study is aimed at minimizing the errors in using a sensitivity evaluation model that could occur when sensitivity analysis method is actively used for design evaluation. To extract words of a contrantion-type model by product, interior space, and streetscape design, primary word refinement was conducted with the words extracted from preceding studies. The analysis revealed that 19 words were used in all three fields. A reliability analysis revealed that different words had a bad impact on the reliability in each field. The applicability was reviewed through reliability analysis of EPA model as contraction-type and PAD model as inference-type. The results are as follows. Although the reliability of the contrantion-type model was higher than that of inference-type model in all three fields, the differences in Cronbach's Alpha were small. Also, When the reliability was analyzed after deleting the words that had a bad impact on reliability, the differences in the reliability's coefficients were clearly significant. Therefore, it is necessary to select words suitable for sensitivity evaluation target and objectivity of the evaluation can be boosted by using a proper model. Analysis of the sensitivity evaluation model suitable for future environmental evaluation should be analyzed with various statistical methods, beyond verification of reliability.

The Research Trends in Fundamental Nursing - Based on the Analysis of Journal of Korean Academy of Fundamental Nursing - (기본간호학 연구동향 - 기본간호학회지 논문분석을 기반으로 -)

  • Jeong Ihn-Sook;Kang Kyu-Sook;Kim Kyung-Hee;Kim Keum-Soon;Kim Won-Ock;Byun Young-Soon;Sohng Kyeong-Yae;Son Yeong-Hee;Yang Sun-Hee;Jo Hyun-Sook
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.8 no.2
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    • pp.132-146
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    • 2001
  • Purpose: This study was to analyze the trends and content of all the research (171 research papers) published in the Journal of Fundamentals of Nursing from 1994, when it began to 2000 using an objective tool developed by the researches, and to identify the direction for the future research in Fundamentals of Nursing. Method: Research published in the Journal of Fundamentals of Nursing from 1994 were reviewed. Result: Most of research (94.2%) was non-degree based and funded research was very limited. The average number of researchers per research study was 2.2 and collaborative research has been gradually increasing. The total number of key words was 331 and the average was 2.6 per research. The major key words were not different from other nursing departments and included home care nursing (10 times), health promotion (8 times), self-care (7 times). self-efficacy (7 times), and homo-dialysis (6 times). Eighty seven percent of the research was quantitative research. and there were very few qualitative studies. Considering theory level, it was found that 40% were factor related research For study design, non-experimental studies were most frequent (66%), and the rate, especially of surveys, has remarkably decreased. Selection of subjects by convenience sampling, was most frequent and there were very few studies that provided the rationale for the calculation of sample size. The major subjects of study were patients (44.8%) with various diagnoses. The subjects usually gave oral consent to take part in that study. Giving information (46.9%) and exercise (26.5%) were common nursing interventions, and physiological indices (16.5%). vital signs (10.3%), physical functioning (8.2%), level of knowledge/skill (7.2%) and level of activity (6.2%) were frequently measured as outcome. Variables questionnaires were the major approach used to collect data, and 57.8% of the research provided the Cronbach alpha to guarantee internal consistency of study instruments. Data were analyzed with computerized statistical packages using, ANOVA (42.0%), T test(39.5%), and chi-square test. For the last seven years, nursing research in Fundamentals of Nursing has gradually improved in both quantify and quality. Conclusion: It was difficult to find any uniqueness or difference compared to other departments of nursing. In fact, because the history of the Journal of Fundamentals of Nursing is rather short, we can expect that there will be further improvement in qualify and content in the future.

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Study about shear bond strength of zirconia core used in dental prosthesis (치과 보철물에 사용되는 지르코니아 코어의 전단결합강도에 관한 연구)

  • Sim, Ji-Young;Kim, Jae-Hong;Kim, Woong-Chul;Kim, Hae-Young;Kim, Ji-Hwan
    • Journal of Technologic Dentistry
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    • v.33 no.4
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    • pp.299-306
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    • 2011
  • Purpose: This study aimed to investigate the shear bond strength by manufacturing the veneering porcelain on the IPS e.max $ZirCAD^{(R)}$ zirconia core, using the layering technique and heat-pressing technique, and to evaluate the clinical stability by comparing to the conventional metal ceramic system. Methods: The Schmitz-Schulmeyer test method was used to evaluate the core-veneer shear bond strength of zirconia core ceramic(IPS e.max $ZirCAD^{(R)}$) and their manufacture recommended two veneering ceramic systems(IPS e.max $ceram^{(R)}$, IPS e. max $ZirPress^{(R)}$). A metal ceramic system(Bellabond $plus^{(R)}$, VITA $VM13^{(R)}$) was used as a control group for the two all ceramic system test groups. The maximum loading and shear bond strength was measured. The average shear strength(MPa) was analyzed with the one-way ANOVA and the Tukey's test(${\alpha}$=.05). The fracture specimens were examined using Microscope to determine the failure pattern. Results: The mean shear bond strengths(SD) in MPa were MBSB control 43.62(2.13); ZBSB 18.65(1.76); ZPSB 18.89(1.54). The shear strengths of the zirconia cores were not significantly different(P>.05). Microscope examination showed that zirconia specimens presented mixed failure, and base metal alloy specimens showed adhesive failure. Conclusion: There was no siginificant different between the layering technique and the heat pressing technique in the veneering methods on the zirconia cores. None of the zirconia core and veneering ceramics could attain the high bond strength values of the metal ceramic combination.

The Effects of Flipped Learning Applied to Home Economics Education on Academic Engagement, Self-Directed Learning Ability and Social Interaction of Middle School Students (가정교과에 적용한 거꾸로 수업이 중학생의 수업참여도, 자기주도학습능력 및 사회적 상호작용에 미치는 효과)

  • Cho, Hee-su;Jang, Yoon-Ok
    • Journal of Korean Home Economics Education Association
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    • v.28 no.4
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    • pp.1-20
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    • 2016
  • This study aims to investigate the effects of flipped learning applied to home economics education on improving middle school students' academic engagement, self-directed learning ability and social interaction. The subjects of this study were 129 third-grade students in middle school. To verify the effects of flipped learning adequately, 129 middle school students divided into two groups have participated in the study over a course of nine weeks. One group was taught in the traditional instructed learning method while the other one in the flipped learning methods. The two groups were taught in 13 sessions in total once or twice a week, and each session took 45 minutes. For pretest and post test, the questionnaire consisting of academic engagement scale, self-directed learning scale and social interaction scale were used. To analyze data, ANCOVA was used to examine significant differences between two groups. The main results of this study were the following : First, students who participated in flipped learning are more improved than those who participate in instructed learning in academic engagement. Second, students who joined in flipped learning are much more improved than those who joined in instructed learning in self-directed learning ability. Third, students who join in flipped learning were much more improved than those who joined in instructed learning in social interaction.

Analysis of Radioactivity in Coal Fly Ash (비산석탄회의 방사능 농도 분석)

  • Shin, Hyun-Sang;Lee, Myung-Ho;Kim, Mi-Kyung;Park, Doo-Wun;Lee, Chang-Woo;Rhee, Dong-Seok
    • Journal of Radiation Protection and Research
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    • v.24 no.4
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    • pp.187-193
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    • 1999
  • The specific radioactivity concentrations in the coal fly ash obtained from heat producing stations in Korea were analyzed and its radiological hazard for reuse in construction purpose was evaluated. The concentrations of uranium isotopes in the real fly ash measured by TBP solvent extraction method and $\alpha$-spectrometer were found to be about 116.1 Bq $kg^{-1}$ for $^{238}U$, 5.01 Bq $kg^{-1}$ for $^{235}U$, and 121.2 Bq $kg^{-1}$ for $^{234}U$, respectively. The activity ratio of $^{234}U/^{238}U$, in the coal fly ash was in $1.04\;{\pm}\;0.03$, which is similar to that of uncontaminated Korean soil in natural conditions (1.14). The specific radioactivities of $^{226}Ra,\;^{232}Th,\;and\;^{40}K$ in the coal fly ash were also determined using $\gamma$-spectrometer with a HPGe detector The results showed that $^{226}Ra,\;^{232}Th,\;and\;^{40}K$ in the coal fly ash were in concentrations of $101.7{\sim}113.9$, $39.5{\sim}54.2\;and\;315.0{\sim}990.6$ Bq $kg^{-1}$, respectively. With the specific radioactivities obtained from $\gamma$-spectrometric measurements of the coal fly ash, its radiological hazard for reuse was evaluated. The result showed that the radioactivity of the coal fly ash was in permissible level.

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Studies on the Fermentation Characteristics of Yogurt Added with Pregelatinized Rice Flour (호화쌀가루를 첨가한 요구르트의 발효특성에 관한 연구)

  • Kim, Soo-Hee;Kim, An-Na;An, Byung-Kyu;Choi, Soo-Keun
    • Culinary science and hospitality research
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    • v.20 no.4
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    • pp.37-48
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    • 2014
  • The purpose of this study is developing a formulation with an optimum sensory point by using yogurt added with rice flour and pregelatinized rice flour(alpha rice flour) optimized by response surface method(RSM). The pH, acidity, sugar content, viscosity and number of lactic acid bacteria during fermentation of two types of yogurt(added with rice flour and pregelatinized rice flour) optimized by RSM were analyzed As the fermentation time of both types of yogurt increased, pH showed decreasing trend. The titratable acidity showed increasing trend as fermentation time increased. Sugar content decreased as fermentation time increased. The reasons are believed to be the sugar decrease during glycolysis and lactic acid fermentation. Viscosity was the highest at 6 hours of fermentation. After 10 hours of fermentation, the viscosity was higher than it was before the fermentation. The number of lactic acid bacteria of yogurt added with rice flour and pregelatinized rice flour was 7.43~9.00 log CFU/mL, which is more than optimum value. Therefore, it is possible to confirm that adding rice flour during yogurt manufacturing increases the number of lactic acid bacteria.

Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows

  • Khan, Muhammad Zahoor;Zhang, Zhichao;Liu, Lei;Wang, Di;Mi, Siyuan;Liu, Xueqin;Liu, Gang;Guo, Gang;Li, Xizhi;Wang, Yachun;Yu, Ying
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.9
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    • pp.1507-1519
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    • 2020
  • Objective: The current research was aimed to profile the transcriptomic picture of the peripheral blood lymphocytes (PBLs) associated with immunity in Chinese Holsteins supplemented orally with coated folic acid during the periparturient period. Methods: The total of 123 perinatal cows were selected for this study and divided into three groups; group A (n = 41, 240 mg/500 kg cow/d), group B (n = 40, 120 mg/500 kg cow/d) and group C (n = 42, 0 mg/cow/d) based on the quantity of folic acid fed. Three samples of PBLs were selected from each folic acid treated group (high, low, and control) and RNA sequencing method was carried out for transcriptomic analysis. Results: The analysis revealed that a higher number of genes and pathways were regulated in response to high and low folic acid supplementation compared to the controls. We reported the novel pathways tumor necrosis factor (TNF) signaling, antigen processing and presentation, Staphylococcus aureus infection and nuclear factor (NF)-kappa B signaling pathways) and the key genes (e.g. C-X-C motif chemokine ligand 10, TNF receptor superfamily member 1A, cluster difference 4, major histocompatibility complex, class II, DQ beta, NF-kappa-B inhibitor alpha, and TNF superfamily 13) having great importance in immunity and anti-inflammation in the periparturient cows in response to coated folic acid treatment. Conclusion: Collectively, our study profiled first-time transcriptomic analysis of bovine lymphocytes and compared the involved cytokines, genes, and pathways between high vs control and low vs control. Our data suggest that the low folic acid supplementation (120 mg/500 kg) could be a good choice to boost appropriate immunity and anti-inflammation as well as might being applied to the health improvement of perinatal dairy cows.

Inhibitory Effect of Angelica gigas Nakai Extract on Nitric Oxide Production in RAW 264.7 Cells (대식세포에서 산화질소 생성에 대한 당귀(當歸) 에탄올 추출물의 억제효과)

  • Jeong, Mi-Young;Park, Hi-Joon;Jeong, Jee-Haeng;Kim, Jin-Young;Kang, Jun-Mo;Lee, Na-Kyeong;Lim, Sabina
    • The Journal of Korean Medicine
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    • v.28 no.2 s.70
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    • pp.155-165
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    • 2007
  • Objective : The Angelica gigas Nakai ethanol extract (AGE) was investigated to compare nitric oxide (NO) production and $NF-{\kappa}B$ activity from RAW 264.7 cells, since NO and nuclear $factor-{\kappa}B$ $(NF-{\kappa}B)$ have been shown to be factors implicated in inflammatory disease. Method : AGE was prepared by extracting medicinal herb with 70% (v/v) ethanol solution. We investigated production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) gene expression by ARE in LPS-stimulated RAW 264.7 macrophage cells. We also investigated inhibition of LPS-induced activation of $NF-{\kappa}B$ on western blot. Result : LPS-induced RAW 264.7 cells increased NO production and iNOS expression. Upon treatment with AGE, nitrite production was significantly inhibited in a concentration-dependent manner compared to the untreated control. AGE inhibited this LPS-induced iNOS mRNA and protein in a dose-dependent manner. AGE markedly inhibited the expression of iNOS mRNA and protein at a concentration of 100 ${\mu}g/ml$. LPS-induced RAW 264.7 cells with AGE blocked inhibitory $factor-{\kappa}B{\alpha}$ degradation. Conclusion :This study shows that AGE seems to attenuate inflammation through inhibition of NO production and iNOS expression by blockade of $NF-{\kappa}B$ activation in LPS-stimulated RAW 264.7 cells.

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Isolation and Characterization of Dextrans Produced by Leuconostoc sp. strain JYY4 from Fermented Kimchi

  • Gu, Ji-Joong;Ha, Yoo-Jin;Yoo, Sun-Kyun
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.4
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    • pp.758-766
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    • 2015
  • Dextran is a generic term for a bacterial exopolysaccharide synthesized from sucrose and composed of chains of D-glucose units connected by ${\alpha}$-1,6-linkages by using dextransucrases. Dextran could be used as vicosifying, stabilizing, emulsifying, gelling, bulking, dietary fiber, prebiotics, and water holding agents. We isolated new strain capable of producing dextran from Korean traditional kimchi and identified as Leuconostoc sp. strain JYY4. Batch fermentation was conducted in bioreactor with a working volume of 3 L. The media was MMY and 15% (w/v) sucrose. Mineral medium consisted of $3.0g\;KH_2PO_4$, $0.01g\;FeSO_4$, $H_2O$, $0.01g\;MnSO_4$, $4H_2O$, $0.2g\;MgSO_4\;7H_2O$, 0.01 g NaCl, $0.05g\;CaCl_2$ per 1 liter deionized water. The pH of media was initially adjusted to 6.0. The inoculation rate was 1.0% (v/v) of the working volume. Temperature was maintained at $28^{\circ}C$. The agitation rate was 100 rpm. The production pattern of dextran was associated with the cell growth. After 24 hr dextran reached its highest concentration of 59.4 g/L. The sucrose was consumed completely after 40 hr. Growth reached stationery phase when sucrose became limiting, regardless of the presence of fructose or mannitol. When the specific growth rate was 0.54 hr-1, utilization averaged 5.8 g/L-hr. The yield and productivity of dextran were 80% and 2.0 g/L-hr, respectively. Dextrans produced by were separated to two different size by an alcohol fraction method. The size of high molecular weight dextran (45% alcohol, v/v), less soluble dextran, was between MW 500,000 and 2,000,000. Soluble dextran (55% alcohol, v/v) was between 70,000 and 150,000. The molecular weight average of total dextran (70% alcohol, v/v) was between 150,000 to 500,000. The enzymatic hydrolyzates of total dextran of ATCC 13146 showed branched dextrans by Penicillium dextranase contained of glucose, isomaltose, isomaltotriose, and isomaltooligosaccharides greater than DP4 (degree of polymerization) that had branch points. Compounds greater than DP4 were branched isomaltooligosaacharides. Hydrolysates by the Lipomyces dextranase produced the same composition of oligosaccharides as those by Penicillin dextranase.