• The Korean Journal of Physiology
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• 제23권1호
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• pp.89-98
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• 1989

New Zealand white 계의 토끼에게 EDC를 사용하여 hemocyanin에 접합시킨 human G-17-I을 면역한 결과 gastrin의 방사면역측정에 적합한 항 gastrin 혈청을 얻는데 성공하였다. 본 실험에서 얻은 항 gastrin 혈청의 역가는 1:25,000이며 human G-17-I과 human big gastrin에 매우 친화력이 높은 반면 다른 위장관 peptide 들과는 교차반응성이 아주 낮았다. 본 항 gastrin 혈청의 effective affinity constant는 $1.77{\times}10^{11}/M$이었다. 또한 총 결합부위(total binding sites)는 255 nM, 이질성지수(heterogeneity index)는 0.84 그리고 average affinity constant는 $0.79{\times}10^{11}/M$이었다. 본 실험에서 얻은 항 gastrin 혈청을 사용하여 사람과 흰쥐의 혈장 gastrin 농도를 방사면역측정법으로 측정한 결과 이미 잘 알려진 결과와 동일한 성적을 얻을 수 있었다. 이러한 결과들을 종합하여 볼때 본 실험에서 얻은 항 gastrin 혈청은 생리적농도로 존재하는 gastrin의 방사면역측정에 적합한 것으로 생각된다.

• 한국산학기술학회논문지
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• 제18권5호
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• pp.400-411
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• 2017

본 연구는 비만여성들의 모션비트 리듬운동의 실효성을 극대화하고 생활체육 현장에 적용을 기대하고자하며 그에 따른 건강증진행위의 향상과 지속적인 운동 참가의 조성방안을 제시하는데 그 목적이 있다. 리듬운동 구성은 리권운동을 기본으로 하여 모션비트를 적용한 모션비트 리듬운동 집단 18명, 모션비트를 적용하지 않은 일반비트 리듬운동 집단 18명, 통제 집단 18명으로 나누어 8주간의 중재 프로그램을 적용하였다. 비만여성들의 모션비트 리듬운동에 대한 효과는 다음과 같은 결론으로 요약할 수 있다. 뇌파 반응의 변화가 건강증진행위에 미치는 영향을 살펴보면, 모션비트 리듬운동 집단의 변화에서는 전전두엽 좌측 뇌파(알파파워)에서 스트레스관리에 대한 건강증진행위에 유의한 영향을 미쳤다. 일반비트 모션비트 집단의 변화에서는 전전두엽 양측 뇌파에서 자아실현에 대한 건강증진행위에 유의한 영향을 미쳤다. 이상의 연구결과를 종합해보면, 8주간의 모션비트를 적용한 운동을 통하여 전전두엽의 긍정적 변화가 스트레스와 관련한 건강관리행위에 재미와 흥미를 극대화시켜줌으로써 비만여성들을 위한 적절하고 효율적인 생활체육 프로그램으로 활용할 수 있을 것이다. 아울러 비만인들의 단순 신체효과를 나타낸 결과에서 벗어나 재미있고 여유로운 운동의 효과를 확보하여 지속적인 운동참가의 조성방안으로 제시될 것이라 기대해 본다.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.780-803
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• 2003

Exposure of skin cells, particularly keratinocytes to various nuclear factor-kappaB ($\textrm{NF}_{-{\kappa}}\textrm{B}$) activators [e.g. tumor necrosis factor-$\alpha$, interleukin-1, lipopolysaccharides, and ultraviolet light] leads to phosphorylation and degradation of the inhibitory protein, $\textrm{I}_{{\kappa}}\textrm{B}$. Liberated $\textrm{NF}_{-{\kappa}}\textrm{B}$ is translocated into the nucleus where it can change or alter expression of target genes, resulting in the secretion of extracellular signaling molecules including melanotrophic factors affecting melanocyte. In order to demonstrate the possible role of $\textrm{NF}_{-{\kappa}}\textrm{B}$ activation on the synthesis of melanotrophic factors from the keratinocytes, the activities of $\textrm{NF}_{-{\kappa}}\textrm{B}$ induced by melanogenic inhibitors (MIs) were determined in human HaCaT keratinocytes transfected with $\textrm{pNF}_{-{\kappa}}\textrm{B}$-SEAP-NPT plasmid. Transfectant cells released the secretory alkaline phosphatase (SEAP) as a transcription reporter in response to the $\textrm{NF}_{-{\kappa}}\textrm{B}$ activity and contain the neomycin phosphotransferase (NPT) gene for the dominant selection marker for geneticin resistance. MIs such as niacinamide, kojic acid, hydroquinone, resorcinol, arbutin, and glycolic acid were preincubated with transfectant HaCaT cells for 3 h and then ultraviolet B (UVB) was irradiated. $\textrm{NF}_{-{\kappa}}\textrm{B}$ activation was measured with the SEAP reporter gene assay using a fluorescence detection method. Of the Mis tested, kojic acid ($IC_{50}$/ = 60 $\mu$M) was found to be the most potent inhibitor of UVB-upregulating $\textrm{NF}_{-{\kappa}}\textrm{B}$ activation in transfectant HaCaT cells, which is followed by niacinamide ($IC_{50}$/= 540 $\mu$M). Pretreatment of the transfectant HaCaT cells with the Mis, especially kojic acid and niacinamide, effectively lowered $\textrm{NF}_{-{\kappa}}\textrm{B}$ binding measured by electrophoretic mobility shift assay. Furthermore, these two inhibitors remarkably reduced the secretion level of IL-6, one of melanotrophic factors, triggered by UV-radiation of the HaCaT cells. These observations suggest that Mis working at the in vivo level might act partially through the modulation of the synthesis of melanotrophic factors in keratinocyte.

• 간호행정학회지
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• 제9권1호
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• pp.89-101
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• 2003

Purpose : this study was to examine the effect of using a nursing standards for the quality of nursing care in musculo-skeletal patients. Method : The approach to nursing quality assurance evaluation was selected process-outcome framework. The subjects ware 100 case of musculo-skeletal patients in orthopedic surgery nursing care unit of E. university hospital in seoul. The data of a control group were collected from 23 December 2001 to 20 March 2002 and an experimental group were collected from 1 June to 25 August 2002. The instruments used for this study were a nursing care standards in musculo-skeletal disorder patients developed by the investigator and an evaluation tool for the quality of nursing care in orthopedic surgery patients developed by soon-ook Choi in 1995. It's reliability is Cronbach alpha=.8628. The data were analyzed by means of $x^1$-test, t-test, ANOVA with SPSS program. Results : This study are as fellows : 1. Scores of the quality of nursing care in standard 1 in the experimental group were higher than those of the control group with no significant difference(t=-8.793, p=.000), scores of the quality of nursing care in standard 2 in the experimental group were higher than those of the control group with significant difference(t=-8.793, p=.000) and standard 3 in the experimental group were higher than those of the control group with significant difference(t=-10.550, p=.000). scores of the quality of nursing care in standard 4 in the experimental group were higher than those of the control group with significant difference(t=-8.793, p=.000). scores of the quality of nursing care in standard 4 in the experimental group were higher than those of the control group with no significant difference(t=-1.833, p=.070). 2. Scores of the quality of nursing care in criteria 28 of 33 criteria in the experimental group were higher than those of the control group with significant difference in 12 criteria(p<.05 ). 3. Scores of the quality of nursing care in standard 1 to standard 4(process evaluation); the experimental group were higher than those of the control group with significant difference(t=-10.704, p=.000). scores of the quality of nursing care in standard 5(outcome evaluation); the experimental group were higher than those of the control group. but no significant difference(t=-1.833, p=.070). 4. Scores of the quality of nursing care in experimental group were higher than those of the control group with significant difference(t=-10.794, p=.000). Conclusion : Through this study, I'm sure that using a nursing care standards in musculo-skeletal disorder patients improve quality of nursing care and nursing care standards are effective nursing care.

• 한국응용곤충학회지
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• 제45권1호
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• pp.31-36
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• 2006

퇴행성 뇌질환은 뇌에 존재하는 면역세포인 소교세포의 염증반응이 발병 요인 중의 하나로 알려져 있다. 이에 본 연구는 초고속자동화시스템(high throughput screening: HTS)을 이용하여 약용곤충추출물로부터 항산화와 항염증 기능이 있다고 알려진 무당벌레 추출물로부터 염증발생인자인 nitric oxide의 생성에 어떠한 영향을 주는지 다음과 같은 결과를 얻었다. 소교세포인 BV-2세포에 대한 무당벌레 추출물의 세포독성은 물과 메탄올, DMSO 추출물에서는 100 ng/ml 까지는 거의 없었으나 에탄올 추출물은 1 ng/ml에서도 세포독성이 있었다. 물과 메탄을 추출물(50 ng/ml)은 LPS로 활성화된 BV-2세포에서 $TNF-{\alpha}$와 $IL-1{\beta}$의 발생을 35-60% 가량 억제 하였다. LPS로 유도된 NO의 생성은 물과 메탄올 추출물을 처리했을 때 각각 55%, 76% 억제되었다. 또한, MeOH 추출물을 처리했을 경우 LPS에 의한 iNOS 발현 정도를 단백질 수준과 mRNA 수준에서 현저하게 억제시킴을 확인하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권21호
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• pp.9327-9333
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• 2014

Breast cancer is the second most common cancer and second leading cause of cancer deaths in women. Phosphatidylinositol-3-kinase (PI3K)/AKT pathway mutations are associated with cancer and phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) gene mutations have been observed in 25-45% of breast cancer samples. Insulin growth factor binding protein-5 (IGFBP-5) can show different effects on apoptosis, cell motility and survival in breast cancer. We here aimed to determine the association between PIK3CA gene mutations and IGFBP-5 expressions for the first time in breast cancer patients. Frozen tumor samples from 101 Turkish breast cancer patients were analyzed with high resolution melting (HRM) for PIK3CA mutations (exon 9 and exon 20) and 37 HRM positive tumor samples were analyzed by DNA sequencing, mutations being found in 31. PIK3CA exon 9 mutations (Q546R, E542Q, E545K, E542K and E545D) were found in 10 tumor samples, exon 20 mutations (H1047L, H1047R, T1025T and G1049R) in 21, where only 1 tumor sample had two exon 20 mutations (T1025T and H1047R). Moreover, we detected one sample with both exon 9 (E542Q) and exon 20 (H1047R) mutations. 35% of the tumor samples with high IGFBP-5 mRNA expression and 29.4% of the tumor samples with low IGFBP-5 mRNA expression had PIK3CA mutations (p=0.9924). This is the first study of PIK3CA mutation screening results in Turkish breast cancer population using HRM analysis. This approach appears to be a very effective and reliable screening method for the PIK3CA exon 9 and 20 mutation detection. Further analysis with a greater number of samples is needed to clarify association between PIK3CA gene mutations and IGFBP-5 mRNA expression, and also clinical outcome in breast cancer patients.

• 한국관광식음료학회지:관광식음료경영연구
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• 제12권1호
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• pp.79-110
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• 2001

Tourist Destination is based on tourism attractions. Components of Tourism attraction are included tourism resources, entertainment facilities, transportation, accommodation, infrastructure, assistance facilities & service, hospitality, information facilities & service, and retailing & service. Tourism resources of them is the key to determine destination development pattern, because tourism attraction that attract tourists is based on tourism resources. Therefore, there are need to study what is tourism attraction of destination at the view of tourists and what is destination development pattern based on it to develop tourism attraction that is able appeal tourists. The purpose of this study is to examine what effect of tourism attraction affects destination development pattern. This study defined Haeundae, Kwanganri, Songjung, Taejongdae in Pusan, Korea as research areas. Research data were collected from 300 respondents by a simple random sampling method. A final 284 usable questionaries were used for empirical analysis after data purification process. Reliability and validity of the scale on the tourism attraction, destination image, and facility needs have been evaluated using Cronbach $\alpha$, item-total correlations. This study analyzed the factors of the tourism attraction and destination images. The result obtained that tourism attraction is divided relaxation attraction, local activity attraction, culture . nature attraction and touring circuit attraction, and destination image is divided culture . urban attractiveness, touring attractiveness, local . stay attractiveness, convenience of travel and relativeness for destination investigated. ANOVA and regression (stepwise) were used to test hypotheses. Based on the results of hypotheses testing, major findings of the empirical research are as follow : 1. The tourism attraction and destination image are significantly different, but facility needs are not significantly by destinations (e. g. Haeundae, Kwanganri, Songjung, Taejongdae) . 2. Destination development pattern is a(fact by the tourism attraction in partial. In case of Haeundea, relaxation attraction take effect partially spa, history and marine/spa tourism. 3. The destination development pattern is influenced by the destination image in partial. In case of Kwanganri, the natural . activity attractiveness and urban tourism images have been found as influential factors that affect marine tourism. 4. The destination images are influenced the physical attributes in literature review, but the destination image are taken effect partially the tourism attraction in this study. 5. Destination development pattern are influenced by the tourism attraction and the destination image partially. This research has provided a variety of practical suggestions. Especially, it was suggested that the destination have appeal to tourists by strengthening attraction and improving weakness. Also, we need to specialize destination in same destination development pattern.

• 한국전문물리치료학회지
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• 제19권4호
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• pp.24-31
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• 2012

The aim of this study was to investigate the effectiveness of self-stretching exercises for iliotibial band (ITB) (Side-lying; right hip and knee were flexed to support the pelvis while left hip was extended and adducted, Standing A; side-bending of the trunk on standing with crossed leg, Standing B; same as Standing A, except the hands were clasped overhead and shifted right side, and Standing C; same as Standing B, except moving the arms diagonally downward) to help determine the most effective self-stretching method to stretch ITB. Twenty-one healthy subjects who do not have ITB shortness from Yonsei University (14 men and 7 women) between the ages of 18 to 28 years voluntarily participated. Ultrasound was performed to measure the thickness of the ITB between the long axis of the ITB and the level parallel to the lateral femoral epicondyle during four self-stretching exercises. All data were found to approximate a normal distribution. We used a one-way repeated-measures analysis of variance (ANOVA) to compare the thickness of the ITB among all self-stretching exercises. The level of significance was set at ${\alpha}$=.05. The ANOVA was followed by Bonferroni's correction. The overall mean of ITB thickness was $1.14{\pm}.4$ mm (${\pm}$ standard deviation) in resting status. The change in the ITB thickness in percentages between the tested position of each self-stretching exercises and resting status was significant (p<.05) (Side-lying $26.62{\pm}10.18%$ with 95% confidence interval [CI]=21.99~31.25%; Standing A $29.46{\pm}16.19%$ with 95% CI=22.09~36.84%; Standing B $44.06{\pm}14.82%$ with 95% CI=37.31~50.81%; Standing C $53.76{\pm}12.1%$ with 95% CI=48.25~59.29%). Results indicated significant differences among four self-stretching exercises except Side-lying versus Standing A (p<.01). Based on these findings, the Standing C self-stretching exercise was the most effective in stretching the ITB thickness among four types of ITB self-stretching exercises. Additionally, the Side-lying self-stretching exercise using gravity to stretch the ITB is recommended as a low-load (low-intensity), long-duration stretch.

• 지역사회간호학회지
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• 제8권2호
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• pp.277-288
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• 1997

The purpose of this study is to identify the behavioral subsystems of the obese adult and contribute to the utility of Johnson's Behavioral System Model. The subjects were 167 obese adults in out-patients clinics of 2 hospitals and health clinics for 4 companies. These data were collected by a structured questionnaire with 52 items from July 1 to Sep. 30, 1995. The instrument used for this study was modified from the DBSM -self reporting instrument (1983) and Grubbs(1980)'s. The reliability of this method was cronbach's ${\alpha}$=.8476 and the construct validity of it was accepted by using a factor analysis. These data were analyzed by frequency, percentage, mean, standard deviation, factor analysis, pearson's correlation coefficient, Kruskal-Wallis with an SPSS PC+ Program. The results of this study were as follows: 1. The 9 behavioral subsystems of the obese were indentified : sex-related, attachment-affiliative I(social), dependency, ingestive, eliminative, restorative, aggressive-protective, attachment-affiliative and II (familial), an achievement behavioral subsystem. 2. Out of 9 subsystems, the highest significant positive correlation between dependency and acheivement subsystem was found(r=.5357, p<.01), The next, attachment-affiliative I and attachment-affiliative II subsystem was correlated significantly (r=.4526, p<.01). Significant positive correlations among sex-related, achivement, attachment-affiliative I, II, dependency, restorative, and aggressive-protective subsystems were found. But, ingestive and eliminative subsystems were not correlated with the above seven subsystems significantly, only the positive correlation between the ingestive and eliminative subsystems was found. 3. The explained variance of the 9 subsystems was explained as 56.1% out of a total of one. The sex-related subsystem was the first factor explaining 16.3% of the total variance and then the next 8 factors which resulted in 39.8%. According to the results of this study, the suggestions were as follows: 1. In the nursing practice, nursing assessmentand intervention of the obese should be in cluded not only in ingestive and eliminative subsystems but also in sex-related, attachment-affiliative I, dependency, restorative, aggressive-protective, attachment -affiliative II, and achievement behavioral subsystem. 2. Concerning instruments, some items to measure the eliminative, dependency, and aggressive-protective behavioral subsystems with relatively reliability are needed. 3. Johnson's concept of a dependency subsystem should be clarified. 4. Correlation among the 7 subsystems, and the ingestive and eliminative subsystems should be clarified.

• 한국게임학회 논문지
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• 제13권3호
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• pp.5-18
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• 2013

본 연구는 3D 입체영상게임의 영상효과와 실재감, 피로도 효과를 검증하기 위해 플레이테스트 실험처치 방법론을 통해 2D게임과 비교하여 효과를 분석하였다. 첫째로 3D게임과 2D게임에 대해 사용자들의 영상경험의 차이를 검증하고자 했던 가설은 영상선명도를 제외하고 실물감, 입체감, 실재감가설 모두 채택되었다. 둘째로 3D게임과 2D게임은 안구피로도와 신체피로도 모두 차이가 없는 것으로 나타났다. 이 연구결과는 그동안 3D 입체영상에서 발생하는 영상왜곡과 피로 유발요인으로 피로도가 발생한다고 하는 기존의 연구결과와는 다른 것이다. 셋째로 3D게임과 2D게임 플레이과정에서 발생한 뇌파의 변화를 측정하여 분석한 결과 EEG 알파파의 평균 진폭은 차이가 없었으나 EEG 베타파는 더 높은 진폭대로 발생하는 것으로 확인되었다. 이 연구는 전통적인 실험연구 방법에 EEG 뇌파측정을 방법론적으로 보완하여 3D 입체영상게임의 사용경험과정에서의 뇌생리학적 변화와 차이를 검증하였다.