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Inhibition of Nitric Oxide Production by ladybug extracts(Harmonia axyridis) in LPS-activated BV-2 cells  

Han Sang-Mi (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Lee Sang-Han (Department of Food Science and Technology, Kyungpook National University)
Yun Chi-Young (Department of Biology, Daejeon University)
Kang Seok-Woo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Lee Kyung-Gill (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kim Ik-Soo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Yun Eun-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Lee Pyeong-Jae (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kim Sun-Yeou (Graduate School of East-West Medical Science, Kyung Hee University)
Hwang Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Publication Information
Korean journal of applied entomology / v.45, no.1, 2006 , pp. 31-36 More about this Journal
Abstract
Inflammation in the brain has known to be associated with the development of a various neurologiacal diseases. The hallmark of neuro-inflammation is the activation of microglia, brain macrophage. Pro-inflammatory compounds including nitric oxide(NO) are the main cause of neuro-degenerative disease such as Alzheimer's disease. In the study, we examined whether Harmonia axyridis extracts inhibit the NO production by a direct method using Griess reagent, western blotting and by RT-PCR(Reverse Transcription-Polymerase Chain Reactionin) the gene expression of inducible nitric oxide synthase(iNOS). Distilled water$(H_2O)$ and methanol(MeOH) extracts of H. axyridis inhibited the protein expression of TNF-a(Tumor Necrosis Factor) and IL-6(Interleukin) in LPS (Lipopolysaccharide) stimulated BV-2 cells at the concentration of 100 ng/ml. Incubation of BV-2 cells with the extracts of $H_2O$ of MeOH inhibited the LPS induced NO and iNOS protein. And this inhibition of iNOS protein is concordant with the inhibition of iNOS mRNA expression. These data suggested that H. axyridis extracts may play a crucial role in inhibiting the NO production.
Keywords
Harmonia axyridis; Mmicroglia; BV-2; Nitric oxide;
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1 Depino, A. M., C. Earl, E. Kaczmarczyk, C. Ferrari, H. Besedovsky, A. Rey, F. J. Pitossi and W. H. Oertel. 2003. Microglial activation with atypical proinflammatory cytokine expression in a rat model of Parkinson's disease. Eur J Neurosci. 18: 2731-2742   DOI   ScienceOn
2 Sola, C., C. Casal, J. M. Tusell and J. Serratosa. 2002. Astrocytes enhance lipopolysaccharide-induced nitric oxie production by microglial cells. Eur J Neurosci 16: 1275 -1283   DOI   ScienceOn
3 McGeer, P. L. and E. G. McGeer. 2001. Inflammation, autotoxicity and Alzheimer disease. Neurobiol Aging. 22: 799-809   DOI   ScienceOn
4 McGeer, P. L. and E. G. McGeer. 2002. Inflammatory processes in amyotrophic lateral sclerosis. Muscle Nerve. 26: 459 - 470   DOI   ScienceOn
5 Motterlini, R., R. Foresti, R. Bassi and C. J. Green. 2000. Curcumin, an antioxidant and anti-inflammatory agent, induces heme oxygenase-1 and protects endothelial cells against oxidative stress. Free Radic Biol Med. 28: 1303 -1312   DOI   ScienceOn
6 Kasckow, J. W., G. Aguilera, J. J. Mulchahey, S. Sheriff and J. P. Herman. 2003. In vitro regulation of corticotropin-releasing hormone. Life Sci. 73: 769-781   DOI   ScienceOn
7 Streit, W. J., M. B. Graeber and G. W. Kreutzberg. 1988. Functional plasticity of microglia: a review. Glia. 1: 301 -307   DOI
8 Sweetman, H. L. 1958. The principle of biological control. W. C. Brown Co., Dubuque, Iowa
9 Ward, A. P, M. Deanna, C. Carsten and P. Mark. 2001. Corticotropin-releasing hormone protects neurons against insults relevant to the pathogenesis of Alzheimer's disease. Neurobiol dis. 8: 492 - 503   DOI   ScienceOn
10 Czlonkowska, A., I. Kurkowska-Jastrzebska, A. Czlonkowski, D. Peter and G. Stefano. 2002. Immune processes in the pathogenesis of Parkinson disease; a potential role for microglia and nitric oxide. Med Sci Monit. 8: 165 - 177
11 Scapagnini, G., R. Foresti, V. Calabrese, A. M. G. Stella, C. Green and R. Motterline. 2002. Caffeic acid phenethyl ester and curcumin: a novel class of heme oxygenase-1 inducers. Mol Pharmacol. 3: 554-561
12 Nakashima, M. N., K. Yamashita, Y. Kataoka, Y. S. Yamashita and M. Niwa. 1995. Time course of nitric oxide synthase activity in neuronal, glial, and endothelial cells of rat striatum following focal cerebral ischemia. Cell Mol Neurobiol. 15: 34 1-349   DOI   ScienceOn
13 Park, K. T. and J. S. Lee. 1998. Review on Insect Resources for Medical Use in Kangwon Province. Korean J. Apiculture. 13: 79-92
14 Park, D. S., M. A. Yoon, M. Z. Xu, H. N. Yu, J. R. Kim, T. S. Jeong and H. Y. Park. 2004. Original Articles: Screening of Anti-atherogenic Substances from Insect Resources. Kor. J. Pharmacogn. 35: 233-238   과학기술학회마을
15 Park, K. T., and Y. C. Park. 1994. Survey on the aphidivous predators for biological control agents. J. Agri. Sci. 36: 109-118
16 Arimoto, T. and G. Bing, 2003. Up-regulation of inducible nitric oxide synthase in the substantia nigra by lipopolysaccharide causes microglial activation and neurodegeneration. Neurobiol Dis. 12: 35 -45   DOI   ScienceOn
17 Emerit, J., M. Edeas and F. Bricaire. 2004. Neurodegenerative diseases and oxidative stress. Biomed Pharmacother. 58: 39-45   DOI   ScienceOn
18 Chen, J. C., F. M. Ho, P. D. L. Chao, C. P. Chen, K. C. G. Jeng, H. B. Hsu, S. T. Lee, W. T. Wu and W. W. Lin. 2005. Inhibition of iNOS gene expression by quercetin is mediated by the inhibition of IkE kinase, nuclear factor-kappa Band STAT1, and depends on heme oxygenase-l induction in mouse BV-2 microglia. Eur J Pharmacol. 521: 9 - 20   DOI   ScienceOn
19 Park, J. Y., J. C. Heo, S. M. An, E. Y. Yoon, S. M. Han, J. S. Hwang, S. W. Kang, C. Y. Yun and S. H. Lee. 2005. High Throughput-comatible screening of Anti-Oxidative substances by insect extract library. Korean J. Food Preserv. 12: 482-488   과학기술학회마을
20 Akiyama, R., S. Barger, S. Barnum, B. Bradt, J. Bauer and G. M. Cole. 2000. Inflammation and Alzheimer's disease. Neurobiol Aging. 21: 383 -421   DOI   ScienceOn
21 Jang, J. S., S. K. Kim, J. B. Jan, J. J. Ahn, H. S. Bae and B. I. Min. 2005. Effect of bee venom on the pro-inflammatory responses in RAW 264.7 macrophage cell lin. J. Ethnopharm. 99: 157 -160   DOI   ScienceOn
22 Yang, N. C., L. H. Lu, Y. H. Kao and L. Y. Chau. 2004. Heme Oxygenase-1 attenuates interleukin-1$\beta$-induccd nitric oxide synthase expression in vascular smooth muscle cells. J Biomed Sci. 11: 799-809
23 Popovic, M., M. Caballero-Bleda, L. Puelles and N. Popovic. 1998. Importance of immunological and inflammatory processes in the pathogenesis and therapy of Alzheimer disease. Int J Neurosci. 95: 203 - 236   DOI   ScienceOn
24 Raivich, G., H. Bluethmann and G. W. Kreutzberg. 1996. Signaling molecules and neuroglial activation in the injured central nervol.s system. Keio J Med. 45: 239-247   DOI   ScienceOn
25 Han, I. O., K. W. Kim, J. H. Ryu and W. K Kim. 2002. p38 mitogen-activated protin kinase mediates lipopolysaccharide, not interferon-gamma, -induced inducible nitric synthase expression in mouse BV-2 microglial cells. Neurosci Lett. 325: 9-12   DOI   ScienceOn
26 Lezoualc'h, F., S. Engert, B. Berning and C. Behl. 2000. Corticotropin-releasing hormone-mediated neuroprotection against oxidative stress is associated with the increase of non-amyloidogenic amyloid beta precusor protein and with the suppression of nuclear factor-kappa. B. Mol Endocrinol. 14: 14 7-159   DOI   ScienceOn