• Radiation Oncology Journal
• /
• v.14 no.4
• /
• pp.265-279
• /
• 1996

Damage produced by radiation elicits a complex response in mammalian cells, including growth rate changes and the induction of a variety of genes associated with growth control and apoptosis. At doses of 10,000 cGy or greater, the exposed individual was killed in a matter of minutes to a couple of days, with symptoms consistent with pathology of the central nervous system(CNS) including degenerative changes. The nature of the damage in irradiated cells underlies the unique hazards of ionizing radiation. Radiation injury to CNS is a rare event in clinical medicine, but it is catastrophic for the patient in whom it occurs. The incidence of cerebral necrosis has been reported as high as 16% for doses greater than 6,000 cGy. In this study, the effect of radiation on brain tissue was studied in vivo. Jun and p53 genes in the rat brain were induced by whole body irradiation of rat with 600Co in doses between 1 Gy and 100 Gy and analyzed for expression of jun and p53 genes at the postirradiation time up to 6 hours. Northern analyses were done using 1.8 Kb & 0.8 Kb-pGEM-2-JUN/Eco RI/Pst I fragments, 2.0 Kb-php53B/Bam HI fragment and ,1.1 Kb-pBluescript SK--ACTIN/Eco RI fragment as the digoxigenin or [${\alpha}^{32}P$] dCTPlabeled probes for Jun, p53 and ${\beta}$-actin genes, respectively. Jun gene seemed to be expressed near the threshold levels in 1 hour after irradiation of $^{60}$Co in dose less than 1 Gy and was expressed in maximum at 1 hour after irradiation of $^{60}$Co in dose of 30 Gy. Jun was expressed increasingly with time until 5 or 6 hours after irradiation of $^{60}$Co in doses of 1 Gy and 10 Gy. After irradiation of $^{60}$Co in dose between 20 Gr and 100 Gy, the expression of Jun was however increased to peak in 2 hours and decreased thereafter. p53 gene in this study also seemed to be expressed near the threshold levels in 1 hour after irradiation of $^{60}$Co in dose less than 1 Gy and was expressed in maximum at 6 hours after irradiation of $^{60}$Co in dose of 1 Gy, p53 was expressed increasingly with time until 5 or 6 hours after irradiation of $^{60}$Co in dose between 1 Gy and 40 Gy. After irradiation of $^{60}$Co in doses of 50 Gy and 100 Gy, the expression of p53 was however increased to peak in 2 hours and decreased thereafter. The expression of Jun and p53 genes was not correlative in the brain tissue from rats. It seemed to be very important for the establishment of the optimum conditions for the animal studies relevant to the responses of genes inducible on DNA damage to ionizing radiation in mammalian cells. But there are many limitations to the animal studies such as the ununiform patterns of gene expression from the tissue because of its complex compositions. It is necessary to overcome the limitations for development of in situ Northern analysis.

• Korean Journal of Environmental Agriculture
• /
• v.19 no.4
• /
• pp.328-331
• /
• 2000

Maize (Zea mays L. cv. kosungjaerae and cv. youngwoljaerae) seeds were irradiated with the dose of $0.5{\sim}20$ Gy by $^{60}Co\;{\gamma}-ray$ radiation to investigate the effect of the low dose ${\gamma}-ray$ radiation on the germination rate, early growth and yield. The low dose radiation was able to improve the germination rate and early growth in maize, but the optimal radiation doses were different depended on kinds of cultivars. High stimulatory effect in early growth of maize was observed in 2 Gy irradiation group of kosungjaerae cultivar and in 12 Gy irradiation group of youngwoljaerae cultivar. The optimal radiation dose for the enhancement of yield and yield components in maize was 8 Gy in kosungjaerae cultivar and $4{\sim}12$ Gy in youngwoljaerae cultivar.

• Journal of Radiation Industry
• /
• v.7 no.2_3
• /
• pp.115-119
• /
• 2013

The density profile measurement technology by gamma transmission has been widely used to diagnose processes in the field of refinery and petrochemical industry. This technology can reveal a clue and position of abnormal phenomenon of industrial processes during their operation. In this paper, the feasibility of the gamma transmission technology for detecting changes in the amount of fluid in a distillation column was evaluated by using Monte Carlo simulations. The simulations assumed that $^{60}Co$ (1.17, 1.33 MeV) sources and NaI (Tl) detectors (${\Phi}5{\times}5cm$) are located in opposite sides of a column and it concurrently moves in vertical direction. To determine the dependency of a spatial resolution on aperture size of a collimator, the simulation model for a tray in a column were simulated with the aperture sizes of 1 and 2 cm. The thickness of the high density area including a tray and fluid was 7.6 cm in the simulation. The spatial resolution of the tray was 8.2 and 8.5 cm, respectively. As a result, it was revealed that the conventional density profile measurement technique is not able to show the deviation of liquid level on a tray in a column.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.35 no.1
• /
• pp.104-108
• /
• 2006

Effect of gamma irradiation on the softening of dried fernbrake at different moist-heating conditions was investigated. Dried fernbrake packaged in controlled atmosphere $(CO_2\;25\%,\;N_2\;75\%)$ was irradiated up to 7 kGy with $\gamma-ray$. Hardness of dried fernbrake was significantly decreased at the irradiation dose of above 5 kGy. Hardness of cooked fernbrake was significantly decreased with increasing irradiation dose level as well as heating temperature and time. $DT_{50}$ value (The heating time required to reach the $50\%$ reduction of hardness) for irradiated fernbrake was shortened according to dose level as well as heating temperature and heating time: $DT_{50}$ value of control: 23 min, 5 or 7 kGy: $2.2\~5.0$ min at $60^{\circ}C$. Also, the activation energy for softening of irradiated fernbrake ($1.85\~1.88$ kcal/mole for $5\~7kGy$) decreased compared to control (4.30 kcal/mole). Moisture content, swelling and rehydration rate of gamma irradiated fernbrake during moist heating increased according to the irradiation doses. Sensory results showed that scores of off-flavor and odor in irradiated fernbrake upto 7 kGy were not significantly different from control. Based on these results, gamma irradiation was effective for the reduction of cooking time and the activation energy for softening of dried fernbrake with increasing dose levels.

• Korean Journal of Food Science and Technology
• /
• v.42 no.2
• /
• pp.136-141
• /
• 2010

This study attempted to determine whether Photostimulated Luminescence (PSL) is applicable for the detection of post-irradiated foods by measuring the PSL photon counts of unirradiated and irradiated dried spices and herbs. A total of 19 dried spices and herbs was irradiated with a $^{60}Co$ $\gamma$-ray source at 1, 5 and 10 kGy followed by measurement of PSL photon. The photon counts of unirradiated samples below 700 correspond to negative. Fifteen samples irradiated over 1 kGy showed photon counts of more than 5,000, indicating irradiation treatment. Intermediate counts (photon count 700-5,000) were observed in irradiated white/black pepper, nutmeg and cinnamon bark at 10 kGy. These results suggest that it is possible to detect whether dried spices and herbs were irradiated by analyzing PSL, with the exception of white/black pepper, nutmeg and cinnamon bark. Irradiated white/black pepper, nutmeg and cinnamon bark containing low levels of minerals were not sensitive to PSL. Therefore, further investigation is sugguested to be performed by Thermoluminescence (TL) analysis or another validated or standardized method.

• Journal of Radiation Protection and Research
• /
• v.29 no.4
• /
• pp.263-268
• /
• 2004

Using the Monte Carlo simulation, a study on the lion-proportionality of the prototype phoswich detector with $2'{\times}2'$ CSI(Tl) and plastic scintillator, which was made by KAERI, has been carried. The defector response functions (DRFs) calculated by simulations were compared with the experimental measurement on the $^{137}Cs\;and\;^{60}Co$. To precisely simulate the DRF for the phoswich, the CSI(Tl) non-proportionality was calculated using the electron response and the simplified electron cascade sequence for treating the photoelectric absorption event. The resulting DRFs of $^{137}Cs\;and\;^{60}Co$ sources obtained by simulations were compared with experiments for verification. For $^{137}Cs$, gamma-ray responses simulated by MCNP5 are generally good agreement with the measured ones. But the DRF of $^{60}Co$ does not match well with the results of experiment in the energy region below second peak due to the coincidence effect of two gamma-rays (1.17 MeV and 1.33 MeV). Through the analysis of the non-proportionality of CsI(Tl) in the prototype phoswich, the improved DRFs considering non-proportionality were produced and the simulation results were verified using the experimental measurements. However, to more precisely reproduce the DRF for the phoswich, further studies in relation to the electron channeling effect and the Doppler broadening effect of a scintillator are still needed as well as considering that effect of the transfer contribution.

• Horticultural Science & Technology
• /
• v.32 no.6
• /
• pp.906-911
• /
• 2014

A new coreopsis cultivar, 'Uridream Pink', was developed by mutation breeding using gamma-ray irradiation. Rooted cuttings of 'Uridream 01', developed at Uriseed Company, were exposed to various gray ranges of gamma-rays from a $^{60}CO$ source for 24 h in 2009 at the Korea Atomic Energy Research Institute. Using gamma-rays with the range of 10-100 Gy, a chimeric mutant with pastel pink flowers was induced at 30 Gy from 'Uridream 01', which blooms red-purple flowers (Red-purple group, 59A). Clones that produced flowers with modified pink color were separated from the chimeric mutant and fixed by more than three rounds of cutting back from 2009 to 2010. The separated mutant clones with modified pink color were found to bloom flowers with marketable color and be free of any abnormal plant characteristics. The typical color of the flowers was pastel pink (Red-purple group, 67B), and the clones were registered as 'Uridream Pink' at the Korea Seed and Variety Service (plant variety protection number: 4410). Although the size of flowers and leaves of 'Uridream Pink' are smaller than those of 'Uridream 01', the number of flowers per plant is greater. 'Uridream Pink' can be used as both a pot and a garden plant because of its long blooming period from late spring to late autumn.

• Nuclear Engineering and Technology
• /
• v.3 no.2
• /
• pp.65-75
• /
• 1971

The gamma-ray shielding effects of magnetite concretes have been measured using a broad beam Co-60 gamma-ray source. Mathematical formulae for a trans-mission ratio-to-shield thickness relation were derived from the attenuation curve obtained experimentally and are I (x) = I (ο) exp(-$\mu$X) exp(1.03$\times$10$^{-1}$ X-3.38$\times$10$^{-3}$ X$^2$＋5.29$\times$10$^{-5}$ X$^3$) when X< 20 cm, I (x) =I (ο) exp(-$\mu$X) exp(4.66$\times$10$^{-2}$ X＋2.12$\times$10$^{-1}$ ) when X＞20 cm. Here I (x) is radiation intensity after passing through a thickness X of absorber, I(o) is the initial radiation intensity, $\mu$ is the linear attenuation coefficient of magnetite concrete and is given by (0.0532$\rho$＋ 0.0083)$^{4)}$ $cm^{-1}$ / in accordance with an earlier study, and X is the thickness of absorber. In addition, a model shield which is a rectangular magnetite concrete box with walls of 8cm thickness walls and internal demensions of 40$\times$40$\times$40 cm was constructed and its shielding effect has been measured. The emergent radiation flux appears to be greater with this configuration than with a slab shield of equal thickness.

• Nuclear Engineering and Technology
• /
• v.49 no.3
• /
• pp.592-597
• /
• 2017

Three plastic scintillators of 4.5 cm diameter and 2.5-cm length were fabricated for comparison with commercial plastic scintillators using polymerization of the styrene monomer 2.5-diphenyloxazole (PPO) and 1,4-bis benzene (POPOP). Their maximum emission wavelengths were determined at 426.06 nm, 426.06 nm, and 425.00 nm with a standard error of 0.2% using a Varian spectrophotometer (Agilent, Santa Clara, CA, USA). Compton edge spectra were measured using three gamma ray sources [i.e., cesium 137 ($^{137}Cs$), sodium 22 ($^{22}Na$), and cobalt 60 ($^{60}Co$)]. Energy was calibrated by analyzing the Compton edge spectra. The fabricated scintillators possessed more than 99.7% energy linearity. Light output was comparable to that of the BC-408 scintillator (Saint-Gobain, Paris, France). The fabricated scintillators showed a light output of approximately 59-64% of that of the BC-408 scintillator.

• Radiation Oncology Journal
• /
• v.8 no.2
• /
• pp.137-144
• /
• 1990

The filter elution technique was used to assay Co-60 $\gamma$ ray-induced DNA single-strand breaks(SSB) in EL 4 mouse leukemia cell and mouse spleen lymphocyte. The lymphocytes were stimulated with lipopolysaccharide (LPS, 20 $\mug/ml$) to label [${^3}H$] thymidine. EL 4 cells and lymphocytes in suspension were exposed at $0^{\circ}C$ to 0 Gy, 1 Gy, 5 Gy,10 Gy of Co-60 radiation and elution procedure was performed at PH 12.1. The number of DNA single-strand breaks increased with increasing doses of $\gamma$ rays. The strand scission factor (SSF) was estimated in each experiment (eluted volume 21 ml). The slope for EL 4 cells was $0.01301\pm0.00096\;Gy^{-1}(n=5)$ and the slope for lymphocytes was $0.01097\pm0.00091\;Gy^{-1}(n=5)$. The slopes were significantly different (P<0.005). Thus EL 4 cells were more sensitive to induction of DNA SSB by ionizing radiation than lymphocytes.