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Effects of Saponin Contained Plant Extracts on Ruminal Fermentation Characteristics and Methane Production (Saponin 함유 식물 추출물의 첨가가 반추위 발효성상과 메탄생성에 미치는 영향)

  • Ok, Ji-Un;Baek, Youl-Chang;Kim, Kyoung-Hoon;Lee, Sang-Cheol;Seol, Yong-Joo;Lee, Kang-Yeon;Choi, Chang-Weon;Jeon, Che-Ok;Lee, Sang-Suk;Lee, Sung-Sil;Oh, Young-Kyoon
    • Journal of Animal Science and Technology
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    • v.53 no.2
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    • pp.147-154
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    • 2011
  • This study was conducted to evaluate the effects of saponin contained plant extracts on in vitro rumen fermentation characteristics and methane production. Ruminal fluid was collected from rumen cannulated Hanwoo steers fed rice straw and concentrate (5:5). Collected rumen fluids, corn starch and buffer including saponin contained plant extracts (ginseng, Ogapi, soapwort, tea plant and yucca; 0.5%/15 ml) were incubated at $39^{\circ}C$ for 24 h. All incubations were repeated five times. Rumen pH in all treatments was lower (p<0.05) compared with that of the control (no addition) during incubation time. The concentration of total VFA in all treatments was higher (p<0.05) than that of the control after 12h incubation. Compared with the control, the concentration of acetate and propionate in all treatments was lower and higher after 6h incubation, respectively. The concentration of $NH_3$-N in all treatments was lower (p<0.05) than that of the control except for Ogapi or yucca extracts supplementation. The number of protozoa in all treatments was significantly (p<0.05) lower than that of the control except for soapwort extract supplementation. The total gas production and methane production in all treatments was higher (p<0.05) and lower (p<0.05) compared with the control, except for ogapi or soapwort extracts supplementation after 12h incubation, respectively. Therefore, reduction in methane production by saponins may could be results from decreased protozoal population without any negative in vitro fermentation.

Analysis of Daily Milking Flow in Holstein Dairy Cow Using the LactoCorder (전자식유량계를 활용한 홀스타인 젖소의 비유형질 분석)

  • Cho, Kwang-Hyun;Choi, Jun-Pyo;You, Byung-Wha;Lee, Deuk-Hwan;Kong, Hong-Sik;Park, Kyung-Do;Lee, Hak-Kyo
    • Journal of Animal Science and Technology
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    • v.51 no.4
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    • pp.265-272
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    • 2009
  • A total of 486 milk records were collected from 16 diary farms in Imsil-gun, Jeollabuk-do. Results obtained were as follows: The average 3MG (amount of milk within the first three minute) was 7.44 kg and 55% of total milk yield was produced within 3 min. The average of SPL (% of foam in milk) was 33.93% and the average of MNG (strip yield) was 0.14 kg, which was less than 1% of total milk yield. The averages of HMF (highest milk flow), HMG (maximum milk flow rate in one minute) and DMHG (average milk flow in the main milking phase) were 3.03 kg/min, 2.94 kg/min and 2.05 kg/min, respectively and the average milking speed in Imsil-gun was slower than other regions. The average of tS500(time to reach 0.5 kg/min at beginning) was 0.23min (about 14 seconds) and that of tMGG (duration of the total milking) was 7.75min. The average tMBG (duration of the dry milking phase) was 0.58 min (35 seconds) and that of tMNG (duration of the stripping phase) was 0.42min (14 seconds). The averages of ELHMF (electrical conductivity at highest milk flow) and ELAP (beginning peak level of the electrical conductivity) were 6.81 mS/cm and 7.58 mS/cm, respectively. The average of ELMAX (maximum electrical conductivity) was 7.48 mS/cm and that of ELAD (beginning peak difference of the electrical conductivity) was 0.61 mS/cm. While the total milk yields for DMHG, tMHG (duration of the main milking phase), tPL (duration of the plateau phase), tAB (duration of the descending phase) and tMGG were positively correlated (0.35~0.54), those for tMBG and SPL were negatively correlated (-0.11 and -0.27). As the DMHG increased, tMHG, tPL, tAB, tMGG and SPL decreased. While the cows with higher electrical conductivity at the beginning of milking had less somatic cell counts, cows with higher electrical conductivity after the peak of milk yield had more somatic cell counts. The results of this experiment indicated that through milking based on milking and lactating standards and the regular checking of milking status, the qualities of milk and milk yields could be improved.

Microwave Irradiation as a Way to Reutilize the Recovered Struvite Slurry and to Enhance System Performance (회수된 MAP 슬러리의 재이용과 공정효율 향상을 위한 도구로서의 극초단파 조사)

  • Cho, Joon-Hee;Lee, Jin-Eui;Ra, Chang-Six
    • Journal of Animal Science and Technology
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    • v.51 no.4
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    • pp.337-342
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    • 2009
  • The feasibility of reutilization of magnesium ammonium phosphate (MAP) or struvite slurry recovered from the process through microwave irradiation was studied in this experiment. For this purpose, 4 different operations were performed with or without Mg source addition and different levels of MAP recycled in a batch reactor. Dissolution rate of MAP, ${NH_4}^+$ elimination pattern and physicochemical changes of MAP during microwave irradiation were also studied. The result showed that only 33% orthophosphate ($PO_4-P$) and 27% $NH_4-N$ removal occurred without adding any external Mg source (run A), whereas 87% $PO_4-P$ and 40% $NH_4-N$ removed when 1.0 M ratio of $MgCl_2$ (run B) was added based on $PO_4-P$ in influent. Although the addition of 1.0 molar ratio of microwave irradiated MAP (Run C) removed lower $PO_4-P$ and $NH_4-N$ than 1.0 M $MgCl_2$ (run B), $PO_4-P$ removal was double when compared with no Mg addition (run A). Addition of half MAP and half $MgCl_2$ (run D) showed the similar removal efficiency (88% $PO_4-P$ and 35% $NH_4-N$) with sole $MgCl_2$ addition (run B). Based on these results, the reutilization of MAP irradiated by microwave would be a feasible way to enhance the removal efficiencies of N and P, as well as curtail the Mg chemical usage. Track study showed that $NH_4-N$ gradually increased at initial stage of microwave irradiation of MAP, and then started eliminating from liquor as temperature increased over $45^{\circ}C$. Dissolution rate of ${PO_4}^{-3}$ during microwave irradiation was proportional to the initial MAP concentration, having $0.0091x^{0.6373}$ mg/sec. It was found from the scanning electron microscope (SEM) study that physical structure of MAP crystal started breaking down into small cube granules within very short time by electromagnetic vibration force during microwave irradiation and then gradually melted down into solution.

The Relationship Between Measurements of Color and Water-Holding Capacity in Pork Loin (돈육 등심의 육색과 보수력 측정치 사이의 상관관계에 관한 연구)

  • Moon, Sang-Hun;Jeong, Jin-Yeon;Kim, Gap-Don;Cho, In-Cheol;Jeon, Jin-Tae;Joo, Seon-Tea;Park, Gu-Boo
    • Journal of Animal Science and Technology
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    • v.51 no.4
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    • pp.329-336
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    • 2009
  • The objective of this study was to investigate the relationship between measurements of color and water-holding capacity (WHC) in pork loin. A total of 419 pork loins were sampled from crossbred (Landrace$\times$Jeju native black pig) F2 pigs at a commercial slaughter house. Meat color measurements (CIE $L^*$, $a^*$, $b^*$), chroma ($C^*$) and hue angle (h) were measured with the Minolta Chromameter and WHC was measured by filter paper fluid, drip loss and cooking loss. Also pH, moisture content and crude fat content were measured at 24 hr postmortem. CIE $L^*$, $b^*$ and h values had positive correlation with drip loss (r=0.52, 0.42), but CIE $a^*$ and $C^*$ values were not related to drip loss. Results showed that CIE $L^*$, $b^*$, and h color system was better than CIE $L^*$, $a^*$, $b^*$ color system to predict WHC, especially drip loss. pH was negatively correlated to drip loss (-0.42) and CIE $L^*$ (-0.67). Although CIE $L^*$ and pH were correlated to drip loss, the accuracy of their estimates for drip loss was 27% and 17%, respectively. Consequently, it was confirmed that meat color and WHC were not perfectly related and suggested that CIE $L^*$, $b^*$, and h color space should not be used independently to predict WHC of pork loin.

Effects of a Mixed Proportion of Alaska Pollack, Chicken Breast Surimi and Starch on Textural Properties of Sausage (명태 및 닭가슴살수리미와 전분의 혼합비율이 소시지의 조직학적 특성에 미치는 영향)

  • Jin, Sang-Keun;Kim, Il-Suk;Yang, Mi-Ra;Hur, In-Chul;Jung, Hyun-Jung;Yang, Han-Sul;Choi, Yeung-Joon
    • Journal of Animal Science and Technology
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    • v.50 no.4
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    • pp.543-550
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    • 2008
  • This study was conducted to determine the effect of different proportions of Alaska pollack surimi, chicken breast surimi and starch on textural properties of sausage. Sausage were manufactured such as Alaska pollack surimi(control), T1(90.91:0:9.09), T2(80:0:20), T3(62.5:37.5:0), T4(58.82:35.3:5.88), T5 (40:60:0) and T6(36.36:54.55:9.09) depending on different proportions of Alaska pollack surimi, chicken breast surimi and starch. Brittleness, breaking force and gel strength were significantly higher in T1 than the control(P<0.05), and breaking force, gel strength and folding test were significantly higher but sensory evaluation was significantly lower in T2 than the control(P<0.05). Breaking force, gel strength and jelly strength were significantly higher in T3 and T4 than the control(P<0.05). Gel characteristics and sensory scores were significantly higher but texture properties were significantly lower in T5 and T6 than the control(P<0.05). The T3 and T4 had similar textural and sensory properties in the surimi- sausage compared to the control. Therefore, the results of this study indicated that textural properties of sausage can be acceptable when chicken breast surimi replaced Alaska pollack up to 35%.

Comparison of Breeding Value by Establishment of Genomic Relationship Matrix in Pure Landrace Population (유전체 관계행렬 구성에 따른 Landrace 순종돈의 육종가 비교)

  • Lee, Joon-Ho;Cho, Kwang-Hyun;Cho, Chung-Il;Park, Kyung-Do;Lee, Deuk Hwan
    • Journal of Animal Science and Technology
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    • v.55 no.3
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    • pp.165-171
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    • 2013
  • Genomic relationship matrix (GRM) was constructed using whole genome SNP markers of swine and genomic breeding value was estimated by substitution of the numerator relationship matrix (NRM) based on pedigree information to GRM. Genotypes of 40,706 SNP markers from 448 pure Landrace pigs were used in this study and five kinds of GRM construction methods, G05, GMF, GOF, $GOF^*$ and GN, were compared with each other and with NRM. Coefficients of GOF considering each of observed allele frequencies showed the lowest deviation with coefficients of NRM and as coefficients of GMF considering the average minor allele frequency showed huge deviation from coefficients of NRM, movement of mean was expected by methods of allele frequency consideration. All GRM construction methods, except for $GOF^*$, showed normally distributed Mendelian sampling. As the result of breeding value (BV) estimation for days to 90 kg (D90KG) and average back-fat thickness (ABF) using NRM and GRM, correlation between BV of NRM and GRM was the highest by GOF and as genetic variance was overestimated by $GOF^*$, it was confirmed that scale of GRM is closely related with estimation of genetic variance. With the same amount of phenotype information, accuracy of BV based on genomic information was higher than BV based on pedigree information and these symptoms were more obvious for ABF then D90KG. Genetic evaluation of animal using relationship matrix by genomic information could be useful when there is lack of phenotype or relationship and prediction of BV for young animals without phenotype.

Effect of Dietary Supplementation of Dried-Citrus Pulp and Wheat Bran on Growth and Meat Quality in Horses (건조감귤박 및 소맥피를 급여한 말의 성장과 육질에 미치는 영향)

  • Chae, Hyun-Seok;Kim, Nam-Young;Cho, In-Chul;Cho, Sang-Rae;Cho, Won-Mo;Park, Yong-Sang;Oh, Shin-Ae;Jang, Aera;Seong, Pil-Nam;Ko, Moon-Suck
    • Journal of Animal Science and Technology
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    • v.55 no.3
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    • pp.219-227
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    • 2013
  • This study was conducted to elucidate the characteristics of equine meat traits and to evaluate the utilization of dried-citrus pulp abundantly produced in Jeju Island as feed ingredient in the diet of finished meat horses. Treatment groups consisted of wheat bran (A) and the dried-pellet mixture of citrus pulp (20%) and wheat bran (80%) (B). Feeding of agricultural by-products were 2 times in a day at the level of 1.5% of body weights. Water and dried-grass forage was not restricted. The average daily gain was similar in both groups. Feed conversion of treatment B showed lower level than that of A. Carcass rate of B was relatively lower than that of A, but meat yield grade of B was higher than that of A. For meat color traits, $L^*$ of B was lighter than that of A, and $b^*$ of group B was lower than that of group A. From the analysis of physical characteristics of equine meats, shearing force had decreased in the treatment B but the levels of drip loss and water holding cavity had increased in the treatment B compared to A. The level of crude fat is lower in the treatment B than the treatment A. For the mineral contents, the treatment B showed higher levels of Ca, K, Mg, Cu and Mn than that of A. For the fatty acid contents, the treatment B showed increased levels of stearic acid, oleic acid, eicosenoic acid, and arachidonic acid compared to A. Consequently, these results suggested that dried-citrus pulp might be utilized as replacement material for equine forage. Please rewrite the whole abstract and should include all required information. Before resubmission, please check it again from a native colleague in your group.

Comparing Farming Methods in Pollutant runoff loads from Paddy Fields using the CREAMS-PADDY Model (영농방법에 따른 논에서의 배출부하량 모의)

  • Song, Jung-Hun;Kang, Moon-Seong;Song, In-Hong;Jang, Jeong-Ryeol
    • Korean Journal of Environmental Agriculture
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    • v.31 no.4
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    • pp.318-327
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    • 2012
  • BACKGROUND: For Non-Point Source(NPS) loads reduction, pollutant loads need to be quantified for major farming methods. The objective of this study was to evaluate impacts of farming methods on NPS pollutant loads from a paddy rice field during the growing season. METHODS AND RESULTS: The height of drainage outlet, amount of fertilizer, irrigation water quality were considered as farming factors for scenarios development. The control was derived from conventional farming methods and four different scenarios were developed based combination of farming factors. A field scale model, CREAMS-PADDY(Chemicals, Runoff, and Erosion from Agricultural Management Systems for PADDY), was used to calculate pollutant nutrient loads. The data collected from an experimental plot located downstream of the Idong reservoir were used for model calibration and validation. The simulation results agreed well with observed values during the calibration and validation periods. The calibrated model was used to evaluate farming scenarios in terms of NPS loads. Pollutant loads for T-N, T-P were reduced by 5~62%, 8~37% with increasing the height of drainage outlet from 100 mm of 100 mm, respectively. When amount of fertilizer was changed from standard to conventional, T-N, T-P pollutant loads were reduced by 0~22%, 0~24%. Irrigation water quality below water criteria IV of reservoir increased T-N of 9~65%, T-P of 9~47% in comparison with conventional. CONCLUSION(S): The results indicated that applying increased the height of drainage after midsummer drainage, standard fertilization level during non-rainy seasons, irrigation water quality below water criteria IV of reservoir were effective farming methods to reduce NPS pollutant loads from paddy in Korea.

Transfer Factor of Heavy Metals from Agricultural Soil to Agricultural Products (농작물 재배지 토양 내 비소, 납 및 카드뮴의 농산물로의 전이계수 산출)

  • Kim, Ji-Young;Lee, Ji-Ho;Kunhikrishnan, Anitha;Kang, Dae-Won;Kim, Min-Ji;Yoo, Ji-Hyock;Kim, Doo Ho;Lee, Young-Ja;Kim, Won Il
    • Korean Journal of Environmental Agriculture
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    • v.31 no.4
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    • pp.300-307
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    • 2012
  • BACKGROUND: The Transfer Factor (TF) of heavy metals from soil to plant is important, because TF is an indicator of heavy metal in soils and a factor that quantifies bioavailability of heavy metals to agricultural products. This study was conducted to investigate the transfer ability of Arsenic (As), Cadmium (Cd), and Lead (Pb) from soil to agricultural products. METHODS AND RESULTS: We investigated heavy metals (As, Cd and Pb) concentrations in 9 agricultural products (rice, barely, corn, pulse, lettuce, pumpkin, apple, pear, tangerin) and soil. TF of agricultural products was evaluated based on total and HCl-extractable soil concentration of As, Cd, and Pb. Regression analysis was used to predict the relationship of total and HCl-extractable concentration with agricultural product contents of As, Cd, and Pb. The result showed that TF was investigated average 0.006~0.309 (As), 0.002~6.185 (Cd), 0.003~0.602 (Pb). The mean TF value was the highest as rice 0.309 in As, lettuce 6.185, pear 0.717, rice 0.308 in Cd, lettuce 0.602, pumpkin 0.536 in Pb which were dependent on the vegetable species and cereal is showed higher than fruit-vegetables in As. CONCLUSION(S): Soil HCl-extractable concentration of As, Cd, and Pb had the larger effects on thier contents in agricultural products than total soil concentrations. We suggests that TF are served as influential factor on the prediction of uptake. Further study for uptake and accumulation mechanism of toxic metals by agricultural products will be required to assess the human health risk and need TF of more agricultural products.

Effect of Potassium Fertigation Level on Growth and Yield of 'Campbell Early' Grapevine (Vitis labrusca L.) in Open Field (노지 포도재배에서 칼륨관비 수준에 따른 과실의 수량 및 품질 변화)

  • Kang, Seok-Beom;Lee, In-Bog;Park, Jin-Myeon;Song, Yang-Ik;Kweon, Hun-Joong
    • Korean Journal of Environmental Agriculture
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    • v.30 no.2
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    • pp.132-137
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    • 2011
  • BACKGROUND: This experiment was carried out to evaluate the effect of potassium fertigation on the growth and yield of fourteen years-old 'Campbell Early' grapevine (Vitis labrusca L.) on a sandy loam soil. METHODS AND RESULTS: Potassium application (125 kg/ha/yr) was top-dressed as 30% of annual potassium application in all treatments and the remainders were drip-irrigated with 0 (0% of total K, K0 level of the remainder), 25 (17.5% of total K, K1/4 level of the remainder), 50 (35% of total K, K1/2 level), and 100mg/L (70% of total K, K1 level) in the intervals of 2 times a week for 12 weeks and the effect of K drip fertigation was compared to control treatment in which the K remainder was applied with surface application as additional fertilizer. The growth of stem diameter, leaf number and shoot length were highest in K1/2 (50 mg/L K), but K of mineral contents was lowest in K0. Yield of grapevine was no difference in 2008, but significantly higher K1/2 than other treatments in 2009. However, fruit quality (color degree, brix, acid contents) was no difference among the treatment. CONCLUSION(s): From the results, It is expected that K1/2 levels of fertigation based on soil testing could be more efficient to get optimum yield and save potassium fertilizer than control (surface application) treatments when grapevine was drip-irrigated at open field condition.