• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.258-261
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• 2006

The addition of zeolite particles enhances the stability of mRNA molecules in a cell-free protein synthesis system. When $20{\mu}g/{\mu}L$ of zeolite (Y5.4) is added to a reaction mixture of cell-free protein synthesis, a substantial increase in protein synthesis is observed. The stabilizing effect of zeolite is most dearly observed in an in vitro translation reaction directed by purified mRNA, as opposed to a coupled transcription and translation reaction. Upon the addition of zeolite in the in vitro translation reaction, the life span of the mRNA molecules is substantially extended, leading to an 80% increase in protein synthesis. The effect of zeolite upon the mRNA stability appears be strongly related to the cation exchange (potassium to sodium) reaction. Our results demonstrate the possibility of modifying this biological process using heterogeneous, non-biological substances in a cell-free protein synthesis system.

• Journal of Periodontal and Implant Science
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• 제34권2호
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• pp.345-356
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• 2004

The triclosan was shown to have anti-microbial and anti-inflammatory effect with inhibition of inflammatory mediators such as prostaglandin $E_2(PGE_2)$. The purpose of this study was to elucidate whether and how $PGE_2$ could be inhibited by triclosan in human gingival fibroblast. Human gingival fibroblast-1 cells (ATCC CRL2014) were pre-treated for 1 hour with triclosan (0.001 ${\mu}/ml{\sim}10$ ${\mu}/ml$) and then stimulated with $TNF-{\alpha}$ (1.0 ng/ml). $PGE_2$ synthesis was evaluated by ELISA and gene expression of COX-1 and COX-2 was evaluated by RT-PCR after $TNF-{\alpha}$, triclosan, and NS-398 (COX-2 inhibitor, 5, ${\mu}M$) and/ or cycloheximide (protein synthesis inhibitor, 2 ${\mu}g/ml$). Triclosan was cytotoxic to human gingival fibroblasts in the concentration higher than 1.0 ${\mu}g/ml$ for longer than 24 hours in tissue culture. The $PGE_2$ synthesis was inhibited by triclosan in dose-dependent manner. Greater COX-2 mRNA suppression was observed with triclosan (0.1 ${\mu}g/ml$) than with $TNF-{\alpha}$ alone, without change in COX-1 gene expression. Inhibitory effects of triclosan on $PGE_2$ synthesis disappeared in presence of cycloheximide. This study suggests that triclosan inhibit prostaglandin $E_2$ at the level of COX-2 gene regulation and require de novo protein synthesis.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2001년도 춘계학술대회 논문집
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• pp.275-278
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• 2001

Research on the forced vibration control of a flexible GFR composite beam using $\mu$-synthesis is performed on this paper. Modal analysis method and modal coordinates are introduced to obtain the state equations of the structural system. Using these equations, Robust control algorithm using $\mu$-synthesis is adopted to suppress the forced vibration of a flexible beam since the designed controller can considered plant uncertainty and external disturbance. Constant disturbance which is generated by shaking the flexible beam as I's natural frequency is effectively rejected by a PZT actuator. Simulations and experiments are carried out with the designed controller and effectiveness of forced vibration suppression strategy is verified by results.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2000년도 추계학술대회 논문집
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• pp.11-14
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• 2000

This paper presents the vibration control of a glass-fiber reinforced thermoplastic composite beam with a distributed PVDF sensor and piezo-ceramic achlator. The three types of different controllen which are PID, H$\infty$ , and p-synthesis ontrollcr are employed to achieve vibration suppression in the transient vibration of composite beam. In the H$\infty$ , controller design, 1st and 2nd natural frequencies are considered in the modeling, because robust control theory which has robustness to struchred uncertainty is adopled Lo suppress the vibration. If the controller designed by H$\infty$ , theory does not satisfy control performance, it is improved by $\mu$ -synthesis method with D-K iteration so that the$\mu$-contoller based on the structured singular value satisfies the nominal performance and robust performance Simulations and experiments were carried out with the designed controllers m order to demonstrate the suppression efficiency of each controller.

• International Journal of Precision Engineering and Manufacturing
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• 제1권2호
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• pp.76-83
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• 2000

A study on the robust control of a composite beam with a distributed PVDF sensor and piezo-ceramic actuator is presented in this paper. $1^{st}$ and $2^{nd}$ natural frequencies are considered in the modeling, because robust control theory which has robustness to structured uncertainty is adopted to suppress the vibration. If the controllers designed by $H_{\infty}$ theory do not satisfy control performance, it is improved by $\mu$-synthesis method with D-K iteration so that the $\mu$-controller based on the structured singular value satisfies the nominal performance and robust performance. Simulation and experiment were carried out with the designed controller and the verification of the robust control properties was presented by results.

• 한국동물학회지
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• 제20권2호
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• pp.93-99
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• 1977

HeLa $S_3$세포에서 MMC에 의해 유발된 DNA회복합성은 농도$(0.05\\sim 0.5\\mu g/ml)$에 따른 증가를 보이지 않고 그 율도 비\ulcorner적 낮아 $0.1\\sim 0.5\\mu g/ml$ 농도에서 조사한 전 세포의 $7\\sim 9%$를 나타내고 있다. 시간 변화에 따른 실험에서는 MMC를 제거한 후 24시간까지 거의 비슷한 율로 DNA회복합성이 계속되고 있다. thymidine 상사체중 BUdR을 전처리한 군에서만이 MMC에 의한 DNA회복합성을 증가시켰다. 그러나 BUdR 또는 IUdR과 MMC를 복합처리 할 경우 시간경과에 따라 정상 DNA합성은 감소된다. 이들 결과는 MMC에 의해 유발된 DNA손상은 빠르고 느린 두단계로 회복됨을 암시하는 것이라 생각된다.

• 한국융합학회논문지
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• 제8권5호
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• pp.1-11
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• 2017

$H_{\infty}$ 제어와 뮤-합성 제어 방법을 사용하여 두 가지 상황, 즉 불확실성이 포함되지 않았을 때와 20% 불확실성이 포함되었을 때, 하에서 스프링-매스-댐퍼 시스템의 진동을 최소화하였다. 두 컨트롤러의 안정성 및 성능 파악을 위해 GM와 PM 값을 사용하여 분석되었다. 중앙 수치 미분법과 RMS 방법을 사용하여 출력 응답의 신호 강도가 비교되었다. 끝으로, 안정성 비교를 위하여 3가지 다른 $W_{per\;f1}$ 가중함수의 경우에 대해 총 40개의 $H_{\infty}$ 제어기와 뮤-합성 제어기 무작위 표본이 생성되었다. 전반적으로, 40개 플랜트 모델에서 얻어진 결과 값의 견고한 안정성과 성능 값에 따르면, 뮤-합성 제어기가 $H_{\infty}$ 제어기보다 진동 관리에 효과적임이 입증되었다.

• 약학회지
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• 제34권5호
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• pp.365-373
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• 1990

The effects of the protein fraction of Panax ginseng on primary cultured chicken embryonic brain cells and DRG cultured with a deficient medium were studied. The protein fraction was further fractionated into four groups according to the molecular weight; larger than 10,000 dalton(fraction A), between 5,000 and 10,000 daltons(fraction B), between 1,000 and 5,000 daltons(fraction C), between 500 and 1,000 daltons(fraction D). All four protein fractions at the concentration of $100\;{\mu}g/ml$ significantly increased the number of the brain cells which promoted the neurite outgrowth. The activity of PDHC in the brain cells was elevated significantly by the protein fraction B at the concentration of $100\;{\mu}g/ml$. It was noted that $100\;{\mu}g/ml$ protein fraction C and D significantly enhanced the synthesis of protein in the brain cells. At the concentration of $100\;{\mu}g/ml$, the protein fraction B enhanced RNA synthesis and the protein fraction A significantly enhanced DNA synthesis in the brain cells. The protein fractions B, C, and D significantly promoted the neurite outgrowth of DRG at the concentration of $100\;{\mu}g/ml$.

• 한국항공우주학회지
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• 제44권1호
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• pp.57-69
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• 2016

폐루프 기준으로 $H_{\infty}$ 제어기를 설계하는 알고리즘에는 3가지(2-ARE, mu-synthesis, LMI) 방법이 있다. 본 논문에서 3가지 $H_{\infty}$ 제어기 설계 알고리즘에 대한 기초 이론을 종합하고, Matlab$^{TM}$에 구현된 함수가 사용될 경우, 실무입장에서 가장 적절하다고 판단되는 $H_{\infty}$ 제어기 설계 알고리즘 제시를 위해 인공위성 자세 제어기 설계 후 결과를 비교 분석하였다: 2-ARE 방법과 LMI 방법은 robust stability, robust performance 및 control authority 측면에서 거의 유사하였으나, LMI 방법에 비해 2-ARE 방법이 weighting 함수설계에 더 민감하였고, mu-synthesis 방법은 다른 2가지 설계 방법에 비해 성능면에서 다소 떨어지고, control authority가 크게 나타났다. 따라서 인공위성 자세제어 설계를 위해 실무 관점에서 LMI 방법이 더 편리한 설계 알고리즘이라는 결론을 얻었다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2004년도 추계학술대회논문집
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• pp.574-577
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• 2004

This paper presents a hybrid system combining lead rubber bearings and hydraulic actuators controlled by a $\mu-synthesis$ method for seismic response control of a cable-stayed bridge. A hybrid system could alleviate some of restrictions and limitations that exist when each system is acting alone because multiple control devices are operating. Therefore, the overall control performance of a hybrid system may be improved compared to each system, however the overall system robustness may be negatively impacted by active device in the hybrid system or active controller may cause instability due to small margins. Therefore, a $\mu-synthesis$ method that guarantees the robust performance is considered to enhance the possibility of real applications of the control system. The performances of the proposed control system are compared with those of passive, active, semiactive control systems and hybrid system controlled by a LQG algorithm. Furthermore, an extensive robust analysis with respect to stiffness and mass matrices perturbation and time delay of actuator is performed. Numerical simulation results show that the performances of the proposed control system are superior to those of passive system and slightly better than those of active and semiactive systems and two hybrid systems show similar control performances. Furthermore, the hybrid system controlled by a f-synthesis method shows the good robustness without loss of control performances. Therefore, the proposed control system could effectively be used to seismically excited cable-stayed bridge which contains many uncertainties.