• 제목/요약/키워드: $\beta$-type

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Microbacterium sp. AL-210이 생산하는 levan fructotransferase의 효소활성에 중요한 아미노산의 동정 (Identification of catalytic acidic residues of levan fructotransferase from Microbacterium sp. AL-210)

  • 성희경;문금옥;최기원;최경화;황경주;김묘정;차재호
    • 생명과학회지
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    • 제17권1호
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    • pp.6-11
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    • 2007
  • 당 분해효소의 family 32 (GH32)에 속하는 $\beta-fructofuranosidase$는 3차구조를 근거로 볼 때 W(L/M)(C/N)DP(Q/N), FRDPK, 그리고 ECP(D/G) 부위를 포함하는 세 군데의 보전적인 영역을 가지고 있다. 이러한 $\beta-fructofuranosidase$ family에 속하는 Microbacterium sp. AL-210 유래 levan fructotransferase (LFTase)의 보전적인 산성 아미노산들의 역할이 특정위치 돌연변이법으로 검사되었다. 각각의 돌연변이체는 대장균인 E. coli BL21 (DE3)균주에서 발현되어 대량 생산되었고, 금속 친화 크로마토그래피법과 FPLC법으로 순수 정제되었다. wild-type LFTase의 효소의 활성은 0.74 unit 인 반면 네 개의 돌연변이체인 D63A, D195N, E245A, E245D 각각은 specific activity를 측정해 본 결과 원 균주와 비교해서 약 100배 정도 감소한 효소활성을 보여 주었다. 이로써 아미노산 변형의 target이 되었던 Asp-63, Aps-195, 그리고 Glu-245가 모두 효소 활성 및 기질과의 결합에 상당히 중요한 역할을 하고 있음이 판명되었다. 이러한 세 부위의 산성 아미노산들은 inulinase, levan fructotransferase와 invertase에 모두 보전적으로 위치 하므로 이들은 $\beta-fructofuranosidase$ family내 에서 공통된 역할을 할 것으로 사료된다.

Bacillus sp. 유래 β-Mannanase의 정제 및 Chromatography에 의한 Xanthan Gum 가수분해물의 분리 (Purification of Bacillus sp. β-Mannanase and Separation of Xanthan Gum Hydrolysate by Chromatography Methods)

  • 박귀근
    • 한국식품영양과학회지
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    • 제32권4호
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    • pp.562-566
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    • 2003
  • DEAE Sepahcel ion exchange chromatography(2.5$\times$42cm)에 의해 Bacillus sp. 유래 $\beta$-Mannanase정제를 수행하였다. 정제효소의 비활성은 17.41 units/mg로서 정제배율은 84.74배를 나타내었다. Carbon column chromatography를 이용하여 0~50%의 ethanol gradient법으로 xanthan gum의 가수분해물을 분리한 결과 fraction number 40~45 및 50~60사이에서 broad한 2개 peak의 가수분해물 pattern을 나타내었다. 가수분해물의 분리도를 확인하기 위하여 TLC를 수행한 결과 fraction No. 40~44에서는 Rf value상 중합도 5에 해당하는 가수분해물이 주축을 이루고 있는 반면 fraction No.50~55에서는 중합도 7의 가수분해물이 주축을 이루고 있음을 확인할 수 있었다. 중합도별 가수분해물의 분리도를 높이기 위해 2차 Sephadex G-25 column chromatography를 수행한 결과 fraction No. 12~15에서 중합도 7의 올리고당과 fraction No. 77~80에서 중합도 5의 가수분해물을 분리 할 수 있었고, 가수분해물의 분리도를 확인하기 위해 2차 TLC를 수행 한 결과 fraction No. 12~15에서는 중합도 7이 주축을 이루고 있으나 일부 소량의 고중합도 가수분해물이 공존하고 있는 것으로 사료되며, fraction No. 77~80에서는 분리능이 높게 중합도 5의 가수분해물이 분리되었다. 이와 같이 분리된 2개의 fractions은 FACE법에 의해 Homo type가수분해물로 동정되었다.

중년여성의 사회적지지, 자기효능감, 지각된 건강상태가 노화불안에 미치는 영향 (The Effects of Social support, Self-efficacy and Perceived health status on Aging anxiety of the Middle-aged women)

  • 정영옥;오효숙
    • 한국보건간호학회지
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    • 제30권1호
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    • pp.30-41
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    • 2016
  • Purpose: This study was conducted to determine the effects of middle-aged women's social support, self-efficacy, and perceived health status on aging anxiety. Methods: Research subjects were 210 middle-aged women between 40 - 60 years living in G-city. Data were collected from July $21^{st}$ 2014 to August $8^{th}$ using a structured questionnaire and analyzed using t-test, One way ANOVA, and multiple regression analysis using the SPSS/WIN 21.0 program. Results: Aging anxiety showed statistically significant differences in religion (t=2.44, p=.015), satisfaction with married life (F=4.39, p=.014), type of leisure activity (F=8.09, p<.001), and number of friends (F=3.23, p=.024). Aging anxiety showed correlation with social support (r=-.21, p=.003), self-efficacy (r=-.46, p<.001), and perceived health status (r=-.33, p<.001). Factors affecting aging anxiety were self-efficacy (${\beta}=-.33$, p<.001), perceived health status (${\beta}=-.23$, p<.001), leisure activity type (${\beta}=-.16$, p=.010), and religious affiliation (${\beta}=-.17$, p=.038). The predictive power of these variables was 25% (F=18.63, p<.001). Conclusion: Nursing intervention strategies for improving self-efficacy, perceived health status, and leisure activity are required to relieve aging anxiety of middle-aged women.

Hydroquinone suppresses IFN-β expression by targeting AKT/IRF3 pathway

  • Kim, Yong;Kim, Han Gyung;Han, Sang Yun;Jeong, Deok;Yang, Woo Seok;Kim, Jung-Il;Kim, Ji Hye;Yi, Young-Su;Cho, Jae Youl
    • The Korean Journal of Physiology and Pharmacology
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    • 제21권5호
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    • pp.547-554
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    • 2017
  • Previous studies have demonstrated the role of hydroquinone (HQ), a hydroxylated benzene metabolite, in modulating various immune responses; however, its role in macrophage-mediated inflammatory responses is not fully understood. In this study, the role of HQ in inflammatory responses and the underlying molecular mechanism were explored in macrophages. HQ down-regulated the expression of interferon $(IFN)-{\beta}$ mRNA in LPS-stimulated RAW264.7 cells without any cytotoxicity and suppressed interferon regulatory factor (IRF)-3-mediated luciferase activity induced by TIR-domain-containing adapter-inducing interferon-${\beta}$ (TRIF) and TANK-binding kinase 1 (TBK1). A mechanism study revealed that HQ inhibited IRF-3 phosphorylation induced by lipopolysaccharide (LPS), TRIF, and AKT by suppressing phosphorylation of AKT, an upstream kinase of the IRF-3 signaling pathway. IRF-3 phosphorylation is highly induced by wild-type AKT and poorly induced by an AKT mutant, AKT C310A, which is mutated at an inhibitory target site of HQ. We also showed that HQ inhibited IRF-3 phosphorylation by targeting all three AKT isoforms (AKT1, AKT2, and AKT3) in RAW264.7 cells and suppressed IRF-3-mediated luciferase activities induced by AKT in HEK293 cells. Taken together, these results strongly suggest that HQ inhibits the production of a type I IFN, $IFN-{\beta}$, by targeting AKTs in the IRF-3 signaling pathway during macrophage-mediated inflammation.

임상실습기간 중 간호대학생의 수면 영향요인: Spielman의 수면장애모델을 근간으로 (Factors Influencing Sleep Patterns during Clinical Practice Weeks among Nursing Students : Based on Spielman's Model)

  • 김미예;최희정
    • Journal of Korean Biological Nursing Science
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    • 제18권4호
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    • pp.203-212
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    • 2016
  • Purpose: The purpose of this study was to identify the sleep patterns of nursing students using self-report questionnaire and physiologic measurement, to examine the factors influencing sleep patterns in based on Spielman's model. Methods: Participants were 119 nursing students who were in the clinical practice period. Self-report questionnaires and actigraphy were used to collect the data. Data were analyzed using descriptive statistics, correlation and regression by the SPSS/WIN 21.0 programs. Results: When sleep was measured by self-report questionnaire, 84 students (70.6%) showed poor sleep quality. The mean sleep efficiency was 82.6%, and 67 students(56.3%) showed low sleep efficiency (less than 85.0%). The factors affecting subjective sleep pattern measured by KMLSEQ were circadian sleep type (${\beta}=.28$, ${\rho}=.003$) and alcohol (${\beta}=.20$, ${\rho}=.031$). The factors affecting total sleep time were sedentary behavior (${\beta}=-.27$, ${\rho}=.003$) and daytime sleepiness (${\beta}=-.33$, ${\rho}$<.001). Conclusion: Many nursing students in their clinical practice period expressed sleep disturbance. Factors affecting the perceived sleep measured by the self-report questionnaire and objective sleep evaluated by physiologic measures were different. The evening type of students perceived poor sleep quality, however, sedentary life style and daytime sleepiness resulted in short sleep time. Therefore, more studies measuring the objective sleep characteristics are needed using subjective and objective characteristics.

Random Amplified Polymorphic DNA (RAPD) Analysis for Extended Spectrum ${\beta}-Lactamase$ Producing Klebsiella pneumonia Isolated from Clinical Specimens in Korea

  • Kim Yun-Tae
    • 대한의생명과학회지
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    • 제12권3호
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    • pp.267-274
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    • 2006
  • Klebsiella pneumoniae is the leading cause of nasocomial infection and the most commonly isolated from clinical specimens. $Extended-spectrum-{\beta}-lactamase$ (ESBL) producing K. pneumoniae infection was associated with a significantly longer duration of hospital stay and greater hospital charges. The purpose of this study is to investigate the antibiotic resistant patterns and the DNA fingerprint types of extended-spectrum ${\beta}-lactamase$ (ESBL) producing K. pneumoniae. 223K. pneumoniae strains were collected from three general hospitals with more than 500 beds in Busan, Korea from September 2004 to October 2005. The minimum inhibitory concentration (MIC) of antibiotics was measured using the Gram negative susceptibility (GNS) cards of VITEK (Vitek system, Hazelwood Inc., MO). Random amplified polymorphic DNA method was used to detect DNA fingerprint of the organisms. Of the 226 K. pneumoniae isolates 65 ESBL-producing K. pneumoniae strains were detected by the Vitek system and confirmed by the double-disk synergy test. All the 65K. pneumoniae strains were resistant cefazolin, cefepime, ceftriaxone and aztreonam, and 83.0% of the organisms were resistant to ampicillin/sulbactam, 66.1% to tobramycin, 67.6% to piperacillin/tazobactam, 61.5% to ciprofloxacin, and 47.6% to trimethoprim/sulfamethoxazole and 43.0% to gentamicin. The RAPD patterns were distincted as 10 types by three random 10-mer primers (208, 272, 277). Among ten type patterns, three types (Ic, IIb, IIIe) were remarkably represented at patient of internal department, nerve surgery department, general surgery department, and neonatal room. These results indicate that RAPD can be useful for DNA of strains typing in the epidemiological investigations. Therefore more investigation are needed in order to prevent the ESBL type-producing K. pneumoniae from spreading resistance to oxyimino cephalosphorin antibiotics.

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Both sitagliptin analogue & pioglitazone preserve the β-cell proportion in the islets with different mechanism in non-obese and obese diabetic mice

  • Yeom, Jin-A;Kim, Eun-Sook;Park, Heon-Seok;Ham, Dong-Sik;Sun, Cheng-Lin;Kim, Ji-Won;Cho, Jae-Hyoung;Yoon, Kun-Ho
    • BMB Reports
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    • 제44권11호
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    • pp.713-718
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    • 2011
  • In this study, the effects of sitagliptin analogue (SITA) or pioglitazone (PIO) treatment on glucose homeostasis and ${\beta}$-cell dynamics in animal models of type 2 diabetes-Akita and db/db mice were evaluated. After 4-6 weeks of treatment, both SITA and PIO were shown to lower non-fasting glucose levels and reduced glycemic excursion in the intraperitoneal glucose tolerance test. In addition, both drugs preserved normal islet structure and the proportion of ${\beta}$-cells in the islets. Compared to the controls, SITA treatment induced a higher ${\beta}$-cell proliferation rate in Akita mice and a lower rate of apoptosis in db/db mice, whereas PIO treatment induced a lower rate of apoptosis in db/db mice and reduced proliferation rates in Akita mice. In conclusion, both SITA and PIO appear to exert some beneficial effects on the islet structure in addition to glycemic control via different mechanisms that involve ${\beta}$-cell dynamics in Akita and db/db mice.

SSP 주파수 진폭변조가 Vasoactive Intestinal Peptide와 $\beta$-endorphin, cGMP에 미치는 영향 (Effects of frequency - amplitude electrical stimulation on sympathetic neurotransmitter and vasoactive intestinal peptide)

  • 최영덕;심규리;장문경
    • The Journal of Korean Physical Therapy
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    • 제14권4호
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    • pp.454-474
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    • 2002
  • Vasoactive intestinal peptide (VIP) is a very potent dilatator and a nonadrenergic, noncholinergic (NANC) neurotransmitter or neuromodulator in the peripheral and the central nervous systems. The mechanisms of action of VIP were examined in aortic circular and in uterine longitudinal smooth muscle strips of the rat. The effects of sympathetic neurotransmitter were investigated in gastric and aortic circular muscle strips of the mouse and the rat. The effects of silver spike point, SSP, low frequency electrical stimulations of VIP, sympathetic neurotransmitter and $\beta$-endorphin were examined in plasma, serum and 24h urine from the healthy volunteer. In gastric smooth muscle strips from the mouse, adrenergic neurotransmitter norepinephrine was inhibitory effected, followed by caused phasic and tonic contraction to the, muscrine receptor agonist carbachol and acetylcholine, respectively. In urine from the healthy volunteer, both norepinephrine and epinephrine were significantly decreased in continue type and low frequency (3 Hz) of SSP electrical stimulations. The contractile responses to S-HT in uterine longitudinal smooth muscle strips of the rats were completely decreased by a VIP 1 $\mu$M. The contractile responses to PGF2$\alpha$ were not decreased by a VIP. In plasma and serum from the healthy volunteer, both VIP and $\beta$-endorphin were significantly increased in continue type and low frequency (3 Hz) of SSP electrical stimulations. Therefore, this study demonstrate that VIP has the capacity to relax vascular or gastric smooth muscles in part by stimulating the generation of NO, and silver spike point low frequency electrical stimulation has the capacity both to decrease sympathetic neurotransmitters and to increase VIP, $\beta$-endorphin.

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Saccharomyces cerevisiae 변이주 세포벽 유래의 베타글루칸의 면역활성 및 Cisplatin과의 병용에 의한 항암 상승작용 (Immunostimulating Activity of Beta-Glucan Isolated from the Cell Wall of Mutant Saccharomyces cerevisiae, and Its Anti-Tumor Application in Combination with Cisplatin)

  • 김완재;윤택준;김동우;문원국;이광호
    • 한국식품영양학회지
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    • 제23권2호
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    • pp.141-146
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    • 2010
  • Cisplatin(cis-diamminedichloroplatium) is one of the most effective anti-cancer drugs being clinically used in the treatment of solid tumors. Despite its therapeutic benefits, its use in clinical practice is often limited because of dose related toxicity. It is known that yeast cell wall beta-glucans possess immuno-modulating properties, which allows for their application in antitumor therapy. IS2 is a kind of beta-glucan derived from the cell wall of mutated Saccharomyces cerevisiae, which exhibits anti-cancer activity in vitro and in vivo. The present study explored the possibility of combination therapy of IS2 with cisplatin. In experimental metastasis of colon26-M3.1 cells, prophylactic intravenous administration of IS-2 in combination with cisplatin effectively inhibited tumor metastasis compared with cisplatin alone or IS-2 treatment in vivo. IS-2 effectively enhanced Th1 type cytokines including IFN-$\gamma$, IL-2, IL-12 and GM-CSF. Simultaneously, this combined treatment inhibited production of Th2 type cytokines compared with control. These results suggested that IS-2 can be applied in combination therapy with anti-cancer drugs to minimize their side effects.

장애아동 어머니의 자아존중감, 가족기능, 사회적 지지가 웰니스에 미치는 영향 (Influence of Self Esteem, Family Function and Social Support on Wellness of Mothers of Children with Disabilities)

  • 김미경;권인수
    • Child Health Nursing Research
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    • 제22권1호
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    • pp.1-10
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    • 2016
  • Purpose: Purpose of this study was to investigate factors influencing wellness, and provide basic data for development of intervention programs to improve wellness in mothers of children with disabilities. Methods: Participants were 147 mothers of children with disabilities from an elementary school and kindergarten for children with disabilities located in J and C cities in G province. Data were collected from February 24 to April 24, 2015 using questionnaires. Results: Mean scores were $3.24{\pm}0.38$ of 5 for wellness, $2.40{\pm}0.41$ of 4 for self-esteem, and $2.87{\pm}0.36$ of 4 for family function. There were significant differences in wellness according to religion, education, and type of education program for the children. There were positive correlations between self-esteem, family function, social support, and wellness. Factors influencing wellness were selfesteem (${\beta}$ =.450), family function (${\beta}$ =.313), type of education program for the children (${\beta}$ =.140), and religion (${\beta}$ =.127). These factors explained 42.7% of the variance in wellness. Conclusion: Findings show that levels of wellness in these mothers are not sufficient to allow them to care for their children and to maintain their own overall health. Therefore to increase wellness of mothers of children with disabilities, intervention strategies that focus on improving self-esteem and family functioning need to be developed.