Browse > Article
http://dx.doi.org/10.3746/jkfn.2003.32.4.562

Purification of Bacillus sp. β-Mannanase and Separation of Xanthan Gum Hydrolysate by Chromatography Methods  

박귀근 (경원대학교 생명공학부 분자ㆍ식품생명공학)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.32, no.4, 2003 , pp. 562-566 More about this Journal
Abstract
A $\beta$-mannanase of Bacillus sp. was purified by DEAE Sephacel ion exchange column chromatography. The specific activity of the purified enzyme was 17.41 units/mg protein, representing an 84.74-folds purification of the original crude extract. For the separation of two types of hydrolysates by the action of purified $\beta$-mannanase, carbon column chromatography, sephadex G-25 column chromatography and thin layer chromatography were accomplished. Main hydrolysates were D.P value 5 and 7 containing of low D.P values. By the method of FACE (Fluorophore Assisted Carbohydrate Electrophoresis), two types of hydrolysates were identified to homo type.
Keywords
Bacillus sp.; $\beta$-Mannanase; xanthan gum; FACE method;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Park GG.1994. Production of mannooligosaccharides by the Penicillium purpurogenum mannanase. J Korean Soc Food Nutr 23: 509-514.   과학기술학회마을
2 Takahashi R, Kusakabe I, Maekawa A, Suzuki T, Murakami K. 1983. Studies on mannanase of Actinomycetes. Japan J Trop Agr 27: 140-147.
3 Lowry OH, Rosebrough NJ, Fan AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-271.
4 Tipson RS, Horton D. 1976. ${\beta}$-1,4-Mannosidic linkage of mannan. In Advances in carbohydrate chemistry and biochemistry. Academic Press, New York. Vol 32, p 299-301.
5 Shin SJ. 1988. Emerging foodborne pathogenes of public health importance. The challenge and prospects for the 21st century in verterinary science 38: 77-83.
6 Shin SY, Park JH. 1997. Activities of oxidative enzymes related with oxygen tolerance in Bifidobacterium sp. J Microbiol Biotechnol 7: 356-359.   과학기술학회마을
7 Kobayashi Y, Echizen R, Mutai M. 1984. Intestinal flora and dietary factors. Processings of the 4th RIKEN Symposium on Intestinal flora. Japan Scientific Press, Tokyo. p 69-70.
8 McCleary BV. 1982. Purification and properties of a mannoside mannohydrolase from guar. Carbohydr Res 101: 74-92.   DOI
9 Hishimoto Y, Fukumoto J. 1969. $\beta$-1,4-Mannosidic linkage of konjak. Nippon Nogeikagaku Kaishi 43: 317-319.   DOI
10 Park GG, Jung GH, Kobayashi H. 1999. Purification and application of earthworm $\alpha$-galactosidase by affinity chromatography. Kor J Appl Microbiol Biotechnol 27: 298-307.   과학기술학회마을
11 Lemke M, Churchill PF, Wetzel RG. 1995. Effect of substrate and cell surface hydrophobicity on phosphate utilization in bacteria. Appl Environ Mocrobiol 61: 913-919.
12 Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428.   DOI
13 Kim JH, Lee TK, Yang HC, Oh DK. 1997. Optimization of medium for $\beta$-mannanaseproduction by Bacillus sp. WS- 42. Kor J Appl Microbiol Biotechnol 25: 212-217.   과학기술학회마을
14 Doyle MP, Roman DJ. 1992. Recovery of Campylobacter jujuni and Campylobacter coli from inoculated foods by selective enrichment. Appl Environ Microbiol 43: 1343-1349.
15 Haenel H, Bending J. 1975. Bifidobacterium role of intestinal flora. In Progresses in Food and Nutrition Science. Porgam Press p 1: 21.
16 Tauxe RV, Hargrett-Bean N, Patton CM, Wachsmuth IK. 1988. Campylobacter isolates in the United States, 1982- 1986. Morbid Weekly Report 37: 1-13.
17 Steeg RF, Hellemons JC, Kok AE. 1999. Synergistic actions fnisin, sublethal ultrahigh pressure, and reduced temperature on bacteria and yeast. Appl Environ Microbiol 65: 4148-4154.
18 Park GG, Chang HG. 1992. Sepration and preparation of galactosylmanno-oligosaccharides from copra galactomannan by mannanase from Penicillium purpurogenum. J Microbiol Biotechnol 2: 204-208.   과학기술학회마을
19 Park GG, Lee SY, Park BK, Ham SS, Lee JH. 1991. Characteristic features of a galactosidase from Penicillium purpurogenum. J Microbiol Biotechnol 1: 90-97.   과학기술학회마을
20 Tsujisaka Y, Hiyama K, Fukumoto. 1972. Guar gum hydrolyzing enzyme in plant. Nippon Nogeikagaku Kaishi 43: 155-160.
21 Isao K, Rihei T, Satoru K, Yoshio S, Kazuo M, Akio M, Takao S. 1985. Strusture of the glucomannooligosaccharides resulting from the hydrolysis of konjac glucomannan produced by a ${\beta}$-mannanase from Streptomyces sp.. Report of Research Projecton Tropical Agricultural Resorces 4:151-161.
22 Dekker RFH, Richard GN. 1976. Advances in carbohydrate chemistry and biochemistry. Hemicellulases: Their occurrence, purification, properties and mode of action 32: 300-301.   DOI