• Journal of Korean society of Dental Hygiene
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• v.12 no.6
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• pp.1203-1211
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• 2012

Objectives : The aim of this study was to investigate the factor-related oral care self-efficacy among the type 2 diabetic patients. Methods : Questionnaire was conducted with 174 Type 2 diabetic patients from 9th January to 9th March in 2012. The following conclusion was obtained as a result of carrying out t-test and one-way ANOVA analysis and multiple linear regression analysis. Results : 1. Analysis of the level of each item concerning oral care self-efficacy showed tooth brushing self-efficacy was $13.3{\pm}2.9$, the highest of all. 2. The factor that was most highly related with oral care self-efficacy was oral health behaviors(${\beta}=0.474$). The other factors were found to be expected duration of diabetes(${\beta}=-0.205$), self-assessed physical health(${\beta}=0.177$) and oral health(${\beta}=0.111$) in such order (p<0.05). Conclusions : Diabetes causes a variety of complications in the mouth, and therefore it is very important to practice oral care activity in order to oral health promotion. This study showed oral care self-efficacy appeared to be the greatest factor of relevance in practicing oral care activity. So, dental hygienist is obliged to keep on motivating so that the patient may maintain the oral care activity for him/herself. Also, a study on various intervention methods to improve oral care self-efficacy should be continued.

• Journal of the Korean Society of Food Culture
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• v.28 no.2
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• pp.186-194
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• 2013

The purpose of this study was to understand the effect of loyalty program in family and fastfood restaurant on relationship quality (trust, commitment) and revisit intention. Based on total 394 samples obtained from empirical research, this study reviewed the reliability and fitness of a research model using the Amos program. The hypothesized relationships in the model were simultaneously tested using a structural equation model (SEM). The proposed model provided an adequate fit to the data, ${\chi}^2$ 102.338 (df 48), GFI .959, RMR .049, NFI .970, CFI .984 SEM resulted that loyalty progam (${\beta}$= .429, t=7.880, p< .001) showed a positive significant effect on trust, and that loyalty program (${\beta}$= .455, t=9.532, p< .001) had a positive significant effect on commitment. Also, both trust (${\beta}$= .450, t=6.595, p< .001) and commitment (${\beta}$= .474, t=5.117, p< .001) had a positive significant effect on revisit intention. In addition, there were moderating effects related to restaurant type (family and fastfood restaurant) in terms of causal relationships between loyalty program, relationship quality and revisit intention. Limitations and future research directions are also discussed.

• Korean Journal of Occupational Health Nursing
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• v.22 no.2
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• pp.112-120
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• 2013

Purpose: The purpose of this study was to identify the influence of interpersonal attitude (I+, I-, U+, U-) on communication competence in care workers for frail elderly. Methods: This study was a cross-sectional survey. The data were collected from 153 care workers for frail elderly using a structured questionnaire. The data were analyzed using multiple regression with the SPSS/WIN 20.0 program. Results: The interpersonal attitude style of subjects was I+U+, and the score of communication competence was 3.53. There were significant differences in interpersonal attitude (I+, I-), and communication competence depending on age, education level, experience of personality type test (yes). Factors influencing on communication competence in care workers were interpersonal attitude (I+, I-, U+) and experience of personality type test with $R^2$ value of 48.5% (F=23.47 p<.001). The most influencing factor was I+ (${\beta}$=.36), followed by I- (${\beta}$=-.22), U+ (${\beta}$=.20), and experience of personality type test (yes) (${\beta}$=.16). Conclusion: It is needed to maintain the interpersonal attitude style (I+U+) of care workers. Continuing education program is needed for increasing communication competence especially for those fifties and over, and experienced care workers. Giving an opportunity for personality test is helpful to increase communication competence in care workers.

• Korean Journal of Food Science and Technology
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• v.27 no.4
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• pp.631-634
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• 1995

Some structural characteristics of hot-water insoluble rice starch [3 varieties of Japonica type and 3 varieties of Tongil type(Japonica-Indica breeding type)]were investigated. The amylose contents of hot-water insoluble starches were 2.7-6.1%. The chain distributions of hot-water insoluble starches were composed of super long chain of above ${\overline{DP}}\;55$ (31.9-38.7%), B chain of ${\overline{DP}}\;40-50$ (12.3-18.0%) and A chain of ${\overline{DP}}\;10-20$ (48.5-50.4%). The chain distributions of hot-water insoluble starches and their ${\beta}-limit$ dextrin were difference between rice varieties and their were differ from those of amylopectin and their ${\beta}-limit$ dextrin. these result suggest that hot-water insolubility of rice starch was showed its characteristic on the varieties and it be responsible for the molecular structure of amylopectin.

• Journal of Periodontal and Implant Science
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• v.49 no.4
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• pp.258-267
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• 2019

Purpose: Increased bone regeneration has been achieved through the use of stem cells in combination with graft material. However, the survival of transplanted stem cells remains a major concern. The purpose of this study was to evaluate the viability of transplanted mesenchymal stem cells (MSCs) at an early time point (24 hours) based on the type and form of the scaffold used, including type I collagen membrane and synthetic bone. Methods: The stem cells were obtained from the periosteum of the otherwise healthy dental patients. Four symmetrical circular defects measuring 6 mm in diameter were made in New Zealand white rabbits using a trephine drill. The defects were grafted with 1) synthetic bone (${\beta}$-tricalcium phosphate/hydroxyapatite [${\beta}-TCP/HA$]) and $1{\times}10^5MSCs$, 2) collagen membrane and $1{\times}10^5MSCs$, 3) ${\beta}-TCP/HA+collagen$ membrane and $1{\times}10^5MSCs$, or 4) ${\beta}-TCP/HA$, a chipped collagen membrane and $1{\times}10^5MSCs$. Cellular viability and the cell migration rate were analyzed. Results: Cells were easily separated from the collagen membrane, but not from synthetic bone. The number of stem cells attached to synthetic bone in groups 1, 3, and 4 seemed to be similar. Cellular viability in group 2 was significantly higher than in the other groups (P<0.05). The cell migration rate was highest in group 2, but this difference was not statistically significant (P>0.05). Conclusions: This study showed that stem cells can be applied when a membrane is used as a scaffold under no or minimal pressure. When space maintenance is needed, stem cells can be loaded onto synthetic bone with a chipped membrane to enhance the survival rate.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.104-110
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• 2004

To determine evolution and genotype of new chromosomal AmpC $\beta$-lactamases among clinical isolates of Enterobacter species, we performed antibiotic susceptibility testing, pI determination, sequencing, and phy-logenetic analysis using developed expression/secretion vector. Six isolates have shown to produce AmpC $\beta$-lactamases. Six genes of AmpC $\beta$-lactamases that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized in pMSG12119. Insert fragment containing the ampC genes was sequenced and found to have an open reading frame coding for 381-amino-acid $\beta$-lactamase. The nucleotide sequence of four ampC genes ($bla_EcloK992004.l$, $bla_EcloK995120.1$, $bla_EcloK99230$, and $bla_EareK9911729$) shared considerable homology with that of chromosomal ampC gene ($bla_EcloMHN1$) of E. cloacae MHN1 (more than 99.6% identity). The sequences of two ampC genes ($bla_EcloK9973$ and $bla_EcloK9914325$) showed close similarity to the chromosomal ampC gene ($bla_EcloQ908R$) of E. clo-acae 908R (99.7% identity). The results from phylogenetic analysis suggested that six ampC genes could be originated from $bla_EcloMHN1$ / or $bla_EcloQ908R$ / MIC patterns and exact pI values of six transformants indicated that the developed expression/secretion vector (pMSG1219) was suitable for the characterization of foreign genes in E. coli strain.

• The Journal of the Korea Contents Association
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• v.14 no.8
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• pp.257-265
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• 2014

The purpose of this study was to examine the relationship of the self-rated peridontal health status of dental patients and the type of their treatment(implant, orthodontic treatment and general treatment) to the quality of life(happiness index), to investigate influential factors for oral health, and ultimately to provide some information on the development of oral health care programs geared toward boosting the happiness index. The subjects in this study were the patients who visited dental hospitals and clinics in Busan and South Gyeongsang Province. As for relationship between the field of treatment and the happiness index, the patients who received orthodontic treatment scored higher in both each itemand all the five items, and the patients who received implant treatment scored lower. Regarding links between self-rated periodontal health status and the happiness index, the patients who had no oral symptoms scored higher in both each item and all the five items, and the respondents whose self-rated health status and self-rated oral health status were both very good scored higher in the happiness index. The preparation of oral health programs that aim to improve the self-rated oral health of patients who visit dental hospitals or clinics is required.

• Childhood Kidney Diseases
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• v.15 no.2
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• pp.125-137
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• 2011

Purpose : Men are generally more prone to chronic renal disease and progression to end stage renal disease than women. The purpose of this study is to prove the effect of gender and sex hormone on renal fibrosis in mice with unilateral ureteral obstruction (UUO) and to elucidate the specific underlying mechanisms. Methods :We compared the expression of ${\alpha}$-smooth muscle actin (${\alpha}$-SMA) in female and male mice with complete UUO (day 7). After this, we estimated the changes of renal fibrosis in the female mice with oophorectomy and in the female mice with oophorectomy and replacement of $17{\beta}$-estradiol, respectively. Results : The level of ${\alpha}$-SMA in the female kidney with UUO was significantly lower than that in the male kidney with UUO. oophorectomy and replacement of $17{\beta}$-estradiol did not change the expression of angiotensin II type 1 (AT1) receptor in the female kidney with UUO, whereas the expression of angiotensin II type 2 (AT2) receptor was significantly more elevated in the intact female (IF) and the oophorectomized female with estrogen (OF+E) than that in the oophorectomized female (OF). The expressions of inducible nitric oxide synthase (iNOS) in the IF and OF+E mice were significantly more elevated than that in the OF mice, which was similar to the expression of AT2 receptor. Conclusion : The female gender is associated with resistance to renal fibrosis in obstructive uropathy and this gender difference may originate from the existence of $17{\beta}$-estradiol, which has an anti-fibrotic effect via upregulation of the AT2 receptor and iNOS.

• Development and Reproduction
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• v.22 no.2
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• pp.155-163
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• 2018

This study aimed to investigate changes in the activity and mRNA expression of plasminogen activators (PAs) induced by $17{\beta}$-estradiol ($E_2$), human chorionic gonadotropin (hCG), and interleukin-$1{\beta}$ ($IL-1{\beta}$) in porcine endometrial cells. Endometrial cells were isolated from the epithelium and cultured to 80% confluence. They were then treated for 24 h with $E_2$ (0.2, 2, 20, and 200 ng/mL), $IL-1{\beta}$ (0.1, 1, 10, and 100 ng/mL), and hCG (0.5, 1, 1.5 and 2 IU/mL). mRNA expressions of urokinase-type (uPA) and tissue-type (tPA) PAs were analyzed using reverse transcription PCR, and activities were measured using a PA activity assay. mRNA expressions of uPA and tPA increased with $E_2$ treatment; however, this was not significant. Similarly, treatment with hCG did not influence the mRNA expressions of PAs. Interestingly, treatment with 0.1 ng/mL $IL-1{\beta}$ significantly reduced the mRNA expression of uPA, but did not affect that of tPA. Treatment with 2, 20, and 200 ng/mL $E_2$ increased PA activity compared with the control group; treatment with 0.1 and 1 ng/mL $IL-1{\beta}$ significantly increased PA activity compared with the other $IL-1{\beta}$ treatment groups, whereas treatment with 10 and 100 ng/mL $IL-1{\beta}$ decreased. Treatment with 2 IU/mL hCG increased PA activity compared with the other treatment groups, although there were no significant differences between the hCG and control groups. In conclusion, the activity and mRNA expression of PAs were differently regulated by the hormone/cytokine and its concentration in porcine endometrial cells. Therefore, understanding PA regulatory mechanisms may help to improve the reproductive potential of domestic animals.

• Journal of Microbiology and Biotechnology
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• v.16 no.6
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• pp.889-895
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• 2006

To investigate the antibiotic-resistant patterns and the gene types of extended-spectrum $\beta$-lactamase (ESBL)-producing Klebsiella pneumoniae, we collected 226 Klebsiella pneumoniae strains from three general hospitals with more than 500 beds in Busan, Korea from September 2004 to October 2005, The minimum inhibitory concentration (MIC) of antibiotics was measured using the Gram-negative susceptibility (GNS) cards of Vitek (Vitek system, Hazelwood Inc., MO, U.S.A.). Of the 226 K, pneumoniae isolates, 65 ESBL-producing K. pneumoniae strains were detected by the Vitek system and confirmed by the double-disk synergy test. TEM (Temoniera) type, SHV (sulfhydryl variable) type, and CTX-M (cefotaxime) type genes were detected by polymerase chain reaction. All 65 K. pneumoniae strains were resistant to ampicillin, cefazolin, cefepime, ceftriaxone, and aztreonam, and 83.0% of the organisms were resistant to ampicillin/sulbactam, 66.1% to tobramycin, 67.6% to piperacillin/tazobactam, 61.5% to ciprofloxacin, and 47.6% to trimethoprim/sulfamethoxazole, and 43.0% to gentamicin. TEM-type ESBLs (TEM-1 type, -52 type) were found in 64.6% (42 of 65) of the isolates, SHV-type ESBLs (SHV-2a type, -12 type, -28 type) in 70.7% (46 of 65) of isolates, and CTX-M-type ESBLS (CTX-M-15 type) in 45% (29 of 65) of isolates. Of the 65 ESBL-producing K. pneumoniae strains, two strains were found to harbor blaSHV-28, which were detected in Korea for the first time. Therefore, more investigation and research on SHV-28 are needed in order to prevent the ESBL type-producing K. pneumoniae from spreading resistance to oxyimino cephalosporin antibiotics.