• Preventive Nutrition and Food Science
• /
• 제21권3호
• /
• pp.236-244
• /
• 2016

In this study, we compared the anti-inflammatory activity of Pinus koraiensis cone bark extracts prepared by conventional extraction and microwave-assisted extraction (MAE). Water extracts and 50% ethanol extracts prepared using MAE were applied to RAW 264.7 cell at 5, 10, 25, and $50{\mu}g/mL$ of concentrations, and tested for cytoxicity. The group treated with $50{\mu}g/mL$ of 50% ethanol extracts showed toxicity. In order to investigate the inhibition of nitric oxide (NO) production in RAW 264.7 cells, extracts of water and ethanol were treated with 5, 10, and $25{\mu}g/mL$ concentrations. The inhibitory activity of water and 50% ethanol extracts groups were determined as 40% and 60% at $25{\mu}g/mL$ concentration, respectively. We found concentration dependent decreases on inducible NO synthase. The inhibitory effect against forming inflammatory cytokines, prostaglandin $E_2$, tumor necrosis factor-${\alpha}$, interleukin (IL)-6, and IL-$1{\beta}$, was also superior in the $25{\mu}g/mL$ treated group than the control group. According to these results, the water extracts and 50% ethanol extracts both inhibited inflammatory mediators by reducing the inflammatory response. Therefore, The MAE extracts of P. koraiensis cone bark can be developed as a functional ingredient with anti-inflammatory activity.

• Journal of Ginseng Research
• /
• 제34권4호
• /
• pp.304-313
• /
• 2010

This study was undertaken to discover the effects and possible mechanisms of the effect of red ginseng extract (RGE) on spontaneous sleep. The effects of a low dose (10 mg/kg) and a high dose (200 mg/kg) of RGE were compared in rats. After recovery from a surgical operation enabling electroencephalograms recordings, rats were administered RGE orally. RGE was administered orally for 1 day or once per day for 5 days in either 10 or 200 mg/kg doses. Polygraphic signs were recorded for 12 h after oral administration of RGE. Both treatment with a large dose (200 mg/kg) of RGE for one day and treatment with either a large or a small dose for 5 days reduced the number of sleep.wake cycles. Daily treatment with RGE (either 10 or 200 mg/kg) for 5 days augmented NREM and total sleep, but reduced wakefulness. Delta wave activity recorded during non-REM (NREM) sleep and REM sleep was increased after one treatment with RGE (either 10 or 200 mg/kg). Delta wave activity during NREM was enhanced after daily treatment with RGE (either 10 or 200 mg/kg) for 5 days. Both alpha and beta subunits of the $\gamma$-aminobutyric acid $(GABA)_A$ receptor were significantly over-expressed in the hypothalamus of the RGE-treated groups. Moreover, the expression of glutamic acid decarboxylase was also increased in the hypothalamus. These results demonstrate that RGE may regulate spontaneous sleep via $GABA_A$ergic systems.

• 대한한방체열의학회지
• /
• 제3권1호
• /
• pp.27-35
• /
• 2004

Today, everybody lives in the stress. So 50% to 80% or 60% to 80% of the modern diseases are estimated what is caused by the stress. And one of the most important point of the stress is a too serious and continuos tension state. Therefore the best way to promotion, persistence, prevention and treatment of the modern man's health will be the relaxion from the tension state. The relax therapy as known as am effective method to the disease which as concerned with stress. that can be adapted to the treatment of anxiety-related diseases, insomnia, hypertension, tension headache, migraine, asthma, preparation of delivery, G-I disorders and menstrual disorders. Until now we guess the change of a relaxed state by the observation to the change of autonomic nervous system(ANS) and EEG. From the point of the ANS, sympathetic nervous system progresses slowly but parasympathetic nervous system increases the activity. In the EEG, both the alpha-wave concerned with the deep physical relaxed state and emotional stability and the theta-wave appeared in the state of meditation are increased. Current studies show that among the biochemical changes, especially serotinin like body hormone is increased by the feeling of the stability or the happiness. So, to see the change of the relaxed state in the image of the D.I.T.I., we used the deviational differences between the pre-relaxed state and the post-relaxed state of 70 people. As a result, the portional deviation of the face is decreased in the image of the D.I.T.I. So it can be thaught that we can determine the changes of the relaxed state by the D.I.T.I.

• 한국재료학회:학술대회논문집
• /
• 한국재료학회 1994년도 추계 학술발표 강연 및 논문 개요집
• /
• pp.1-1
• /
• 1994

;The electronic state calculations for various types of ceramic materials have beell performed by the use of $DV-X\;{\alpha}$ cluster method. The molecular orbital levels and wave functions for model clusters have been computed to study the electronic properties ami chemical bonding of the ceramics. For ${\beta}-sialon(Si_{6-z}Al_zO_zN_{8-z})$ which is a high temperature structural material based on ${\beta}-Si_3N_4$, we have made model cluster calculations to estimate the strength of chemical bonding between atoms by the Mulliken population analysis. It is found that the covalent bonding between Si and N atoms is very strong in pure ${\beta}-Si_3N_4$, but the covalency around solute atom is considerably weakened when Si atom is substituted by AI. This tendency is enhanced by an additional substitution of oxygen atom for N. The result calculated can well explain the experimental data of changes in mechanical properties such as the reductions of Young's modulus and Vickers hardness with increment of z-value in ${\beta}-sialon$. Various model clusters for transition metal oxides which show many interesting physical and chemical properties have also been calculated. High-valent perovskite-type iron oxides EMFe0_3E(M=Ca and Sr) possess very interesting magnetic and chemical properties. In these oxides, iron exists as $Fe^{4+}$ state, but the experimental measurement of Mossba~er effect suggests that disproportionation $2Fe^{4+}=Fe^{3+}+Fe^{5+}$ takes place for $CaFe0_3$ at low temperatures. The model cluster calculations for these compounds indicated the existence of considerably strong covalent bonding of Fe-O. The calculations of hyperfine interaction at iron neucleus show very good agreement with the experimental Mossbauer measurements. The result calculated also implies that the disproportionation reaction is strongly possible by assuming the quenching of breathing phonon mode at low temperatures.tures.

• Korean Journal of Acupuncture
• /
• 제29권3호
• /
• pp.467-475
• /
• 2012

Objectives : The aim of this study was to examine the effects of Acupuncture at the GV20 and GV22 on normal human beings using power spectrum analysis. Methods : Electroencephalogram(EEG) power spectrum exhibits site-specific and state-related differences in various frequency bands. 8 channels Background Electroencephalogram (EEG) was carried out in 30 subjects(24 females and 4 males). Results : In ${\delta}$(theta) band, the power values decreased significantly at the 8-channel average value(p=0.03) and especially at T3(p=0.02), T4(p=0.001) and P3(p=0.03). In ${\alpha}$(alpha) band, the power values have no significant changes. In ${\beta}$(beta)band, the power values increased significantly at the 8-channel average value (p=0.02) and especially at T4(p=0.003), P3 (p= 0.03) and P4(0.02). In ${\beta}/{\delta}$(beta/theta) ratio, the value increased significantly at the 8-channel average value(p=0.002) and especially at Fp2(p=0.05), F4(p=0.007), T3(0.012), T4(0.005), P3 (0.007) and P4(0.03) Conclusions : Through this data, we conclude that acupuncture at the GV20 and GV22 on normal human beings could have possibility to awake the cerebral cortex by the functional mechanism.

• 한국식품과학회지
• /
• 제39권1호
• /
• pp.1-6
• /
• 2007

이취성분의 하나인 헥사날이 CD 종류, CD 농도, 저장기간에 따라 결합하는 정도를 전자코를 이용하여 분석하였다. 여러 종류의 CD 중에서 ${\alpha}-CD$가 헥사날과 가장 많은 양이 반응하면서 CD복합체를 이루었다. CD의 농도가 증가할수록 잔존하는 헥사날의 양이 감소하였는데 5% ${\beta}-CD$ 첨가시 86%의 헥사날이 감소하여 헥사날-CD복합체를 이루고 있음을 보여 주었다. CD복합체를 이룬 후 저장초기 상태에서는 헥사날의 양 변화에 별다른 차이가 없었지만 저장일이 지남에 따라 헥사날의 양이 점차 감소하였다. 또한 저장기간이 지남에 따라 자연 휘발되는 양보다는 CD와의 결합에 의해 헥사날의 양의 감소효과가 더 큰 것을 알 수 있었으며 이런 정도를 전자코로 분석할 수 있었다.

• The Korean Journal of Physiology
• /
• 제25권2호
• /
• pp.147-158
• /
• 1991

The effects of noradrenaline on the spontaneous contraction recorded from a strip of mucosa-free antral circular muscle were studied in the guinea-pig stomach, and the changes in slow waves and membrane resistance were analyzed in order to elucidate the mechanism for the excitatory response to noradrenaline. Electrical responses of circular muscle cells were recorded using glass microelectrodes filled with 3 M KCI. Electrotonic potentials were produced to estimate membrane resistance by the partition stimulating method. All experiments were performed in tris-buffered Tyrode solution which was aerated with 100% $O_2$ and kept at $35^{\circ}C$. The results obtained were as follows: 1) The spontaneous contractions were potentiated dose-dependently by the application of noradrenaline. 2) Through the experiments using adrenoceptor-blockers, the strong excitatory effect via $[\alpha}-adrenoceptors$ and the weak inhibitory efffect via ${\beta}-adrenoceptors$ were noted. 3) Noradrenaline produced hyperpolarization of membrane potential, and increases in the amplitude and the maximum rate of rise of slow waves. 4) In the presence of apamin, Ca-dependent K channel blocker, the characteristic hyperpolarization was not developed. However, the excitatory effect of noradrenaline on spontaneous contraction remained. 5) Membrane resistance was reduced during the hyperpolarized state by the application of noradrenaline, and the change of membrane resistance and the hyperpolarized state were completely abolished by apamin. From the above results, following conclusions could be made: Excitatory responses to noradrenaline result from the dominant ${\alpha}-excitatory$, and the weak ${\beta}-inhibitory$ action of noradrenaline. Hyperpolarization of membrane potential by noradrenaline is due to the activation of Ca-dependent K channel.

• 한국자기학회지
• /
• 제1권1호
• /
• pp.1-5
• /
• 1991

다결정${(Fe_{2}O_{3})}_{1-x-y}{(In_{2}O_{3})}_{x}{(Eu_{2}O_{3})}_{y}$(x=0.01, y=0.02과 x=0.02, y=0.03)의 자기적 성질을 X선 회절, $M\"{o}ssbauer$ 효과 방법과 자기이력 측정에 의해서 연구하였다. X선 회절결과는 이들 시료의 결정구조는 $\alpha-Fe_{2}O_{3}$와 같음을 보인다. 사중극자 분열과 평균 반폭치 분석으로부터 x=0.01과 y=0.02인 시료는 Morin 전이를 보이며, x=0.02와 y=0.03시료에서 반 강자성 벡타와 결정축[111]사이의 각은 온도의 증가에 따라 약 $35^{\circ}$로부터 (111) 평면에 놓이기 까지 변한다. 초미세 자기장의 온도 의존성은 스핀파 이론을 써서 분석하였다. 상온에서의 이성질체 이동값은 약 0.35mm/s로 이는 시료내의 철의 이온값은 $3^{+}$임을 의미하는 것이다. 이성질체 이동의 온도의존성은 Delbye모형을 써서 분석하였다.

• 한국게임학회 논문지
• /
• 제15권2호
• /
• pp.19-32
• /
• 2015

실재감, 몰입, 각성은 비디오가 제공하는 흥미로운 사용자 경험이다. 사용자들은 개인의 관심에 응해줄 수 있는 미디어에 대한 요구가 강해지고 있다. 본 연구는 이러한 사용자의 요구를 바탕으로 설문조사방법과 뇌파측정을 결합하여 비디오 시청과 게임을 비교한 연구이다. 연구결과, 비디오 게임이 비디오 시청에 비해 실재감, 몰입, 각성이 높은 수준으로 확인되었으며, 몰입의 상태와 관련이 있다고 알려진 뇌파 역시 높은 수준으로 확인되었다. 마지막으로 게임몰입에 영향을 주는 요인으로 실재감, 각성, 뇌파(${\alpha}$, ${\beta}$, ${\delta}$)변인이 영향을 주는 것으로 확인되었다. 이 연구는 자기보고와 더불어 뇌파측정을 통해 실증적으로 분석하고 관련분야의 이론적, 방법론적 발전에 기여하고자 하였다.

• Korean Journal of Acupuncture
• /
• 제21권2호
• /
• pp.1-28
• /
• 2004

Objectives : Human physiological changes in the state of qigong has been measured using EEG(Electroencephalography), functional MRI(functional Magnetic Resonance Image), EAV(Electro-Acupuncture according to Voll) and SQUID(Superconducting Quantum Interference Device) measurements. Methods & Results : EEGs were measured to study the differences between Qigong masters and Qi receiver on the changes of EEG. During Qigong, an alpha waves were increased. The power spectra indicate that the peak frequency of alpha waves increased during Qigong. Qi receiver's EEG signals seemed to affected by the state of himself. Brain activation did not observed when qigong master concentrates the Qi at Laogong(P8). But a localization of fMRI signal in the sensory cortex was observed by electric acupuncture stimulation at Laogong(P8). Five phase deviation of EAV were clearly changed in the both cases of Qigong master and Qi receiver. When a Qigong master concentrates the Qi at Yintang, Laogong(P8), Qihai(CV6) meridian points during Qigong state, the change of magnetic field around acupoints Yintang, Laogong points has been measured using 40-Channel DROS-SQUID apparatus. After smoothing process of the continuously measured magnetic signal around acupoints for a few minutes, we could observe that a series of peaks, magnitude of -1.0~2.5pT appeared. But there was no significant difference in changes of magnetic signal around acupoints. Physical signals of magnetocardiogram has been measured by using 2-Channel DROS SQUID(Magnetocardiogram). Physical signals of magnetocardiogram were clealy changed at the ST segments after S-wave when qigong master concentrates the Qi.