• Title/Summary/Keyword: $[U-^{14}C]\

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Effect of C18-polyunsaturated Fatty Acids on Their Direct Incorporation into the Rumen Bacterial Lipids and CLA Production In vitro

  • Choi, S.H.;Song, M.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.4
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    • pp.512-515
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    • 2005
  • An in vitro study was conducted to determine the effect of C18-polyunsaturated fatty acid on direct incorporation into the rumen bacteria, bio-hydrogenation and production of CLA in vitro. Sixty milligrams of linoleic acid ($C_{18:2}$) or linolenic acid ($C_{18:3}$) were absorbed into the 0.5 g cellulose powder was added to the 150 ml culture solution consisting of 120 ml McDougall's buffer and 30 ml strained rumen fluid. Four uCi of 1-$^{14}C_{18:2}$ or 1-$^{14}C_{18:3}$ (1 uCi/15 mg each fatty acid) were also added to the corresponding fatty acids to estimate the direct incorporation into the bacterial lipids. The culture solution was then incubated anaerobically in a culture jar with stirrer at 39$^{\circ}C$ for 12 h. Ammonia concentration and pH of the culture solution were slightly influenced by the fatty acids. Amount of fatty acid incorporated into the bacteria was 1.20 mg and 0.43 mg/30 ml rumen fluid for $C_{18:2}$ and $C_{18:3}$, respectively during 12 h incubation. Slightly increased CLA (sum of cis-9, trans-11 and cis-10, trans-12 $C_{18:2}$) was obtained from the $C_{18:3}$ addition compared to that from $C_{18:2}$ after 12 h incubation in vitro.

Crystal Structure Analysis of Uranium Oxides (산화우라늄의 결정구조 분석)

  • 김정석;최용남;이창희;김시향;이영우
    • Journal of the Korean Ceramic Society
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    • v.38 no.11
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    • pp.967-972
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    • 2001
  • The crystal and defect structures of U $O_{x}$(x=2.0, 2.03, 2.14, 2.19, 2.20 and 2.26) were analysed by rietveld refinement and the results were compared to the U-O phase diagram. Neutron diffraction data were collected in the temperature range of RT~100$0^{\circ}C$. The specimens of x=2.14, 2.19, and 2.20 consisted of two phase: $UO_{2+x}$(Fm3m, a≒5.4$\AA$) and $U_4$$O_{9}$(I43d, a≒21.8$\AA$). The proportion of the $UO_{2+x}$(Fm3m) phase increased with increasing the temperature. The variation of the proportion of the two phases with temperature in the U $O_{2.2}$ and U $O_{2.18}$ samples showed some deviation from the expected values from the phase diagram especially at the high temperature range. The phase transitions ${\gamma}$longrightarrow$\beta$longrightarrow$\alpha$ of $U_4$$O_{9}$ were discussed in relation with the phase separation.eparation.ion.

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The Sterol Components of Undaria Pinnatifida and the Incorporation of $^14C-1-acetate$ into Them (미역의 스테롤 조성과 $^14C-1$-식초산염의 스테롤 전환에 관하여)

  • JOH Yong-Goe;HATA Mitsuo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.10 no.3
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    • pp.163-170
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    • 1977
  • The present study was carried out to knew the sterol components of U. pinnatifida and their incorporation abilities of $^14C-1-acetate$ injected into it. The results obtained are as follows: 1. The total lipids are classified as hydrocarbon $1.6\%$, pigment and sterol ester $2.5\%$, triglyceri do $3.3\%$, free fatty acid $2.2\%$, free sterol $3.8\%$, chlorophyll $18.8\%$, and polar lipids $ 67.3\%$ 2. The sterol mixture from U. pinnatifida are omposed of cholesterol $3.5\%$, 24-methylene-cholesterol $11.2\%$, fucosterol $85.3\%$. 3. The radioactivities of the lipids classes from U. pinnatifida injected with $^14C-1-acetate$ are distributed 4,648 dpm/ug in total lipid, 2,754 dpm/mg in polar lipids, 373 dpm/mg in chlorophyll, 22,481 dpm/mg in free sterol, 6,520 dpm/mg in free fatty acid, 789 dpm/mg in sterol ester and 358 dpm/mg in hydrocarbon respectively. 4. The specific radioactivities of the sterols are 115 dpm/mg in cholesterol, 147,821 dpm/mg in 24-methylenecholesterol, 20, 887 dpm/mg in fucosterol.

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Important Radionuclides and Their Sensitivity for Ground water Pathway of a Hypothetical Near-Surface Disposal Facility

  • Park, J. W.;K. Chang;Kim, C. L.
    • Nuclear Engineering and Technology
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    • v.33 no.2
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    • pp.156-165
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    • 2001
  • A radiological safety assessment was performed for a hypothetical near-surface radioactive waste repository as a simple screening calculation to identify important nuclides and to provide insights on the data needs for a successful demonstration of compliance. Individual effective doses were calculated for a conservative ground water pathway scenario considering well drilling near the site boundary. Sensitivity of resulting ingestion dose to input parameter values was also analyzed using Monte Carlo sampling. Considering peak dose rate and assessment time scale, C-14 and T-129 were identified as important nuclides and U-235 and U-238 as potentially important nuclides. For C-14, the dose was most sensitive to Darcy velocity in aquifer The distribution coefficient showed high degree of sensitivity for I-129 release.

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POSITIVE SOLUTIONS FOR NONLINEAR m-POINT BVP WITH SIGN CHANGING NONLINEARITY ON TIME SCALES

  • HAN, WEI;REN, DENGYUN
    • Journal of applied mathematics & informatics
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    • v.35 no.5_6
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    • pp.551-563
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    • 2017
  • In this paper, by using fixed point theorems in cones, the existence of positive solutions is considered for nonlinear m-point boundary value problem for the following second-order dynamic equations on time scales $$u^{{\Delta}{\nabla}}(t)+a(t)f(t,u(t))=0,\;t{\in}(0,T),\;{\beta}u(0)-{\gamma}u^{\Delta}(0)=0,\;u(T)={\sum_{i=1}^{m-2}}\;a_iu({\xi}_i),\;m{\geq}3$$, where $a(t){\in}C_{ld}((0,T),\;[0,+{\infty}))$, $f{\in}C([0,T]{\times}[0,+{\infty}),\;(-{\infty},+{\infty}))$, the nonlinear term f is allowed to change sign. We obtain several existence theorems of positive solutions for the above boundary value problems. In particular, our criteria generalize and improve some known results [15] and the obtained conditions are different from related literature [14]. As an application, an example to demonstrate our results is given.

Effect of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-tang) on Hair Regrowth and Cytokine Changes on Hair-Removed C57BL/6 Mice (익기보혈탕(益氣補血湯)이 C57BL/6 마우스의 육모(育毛)촉진 및 Cytokine 변화에 미치는 영향)

  • Hong, Jung-Ae;Song, Mi-Yeon;Choi, In-Hwa;Sohn, Nak-Won;Chung, Seok-Hee
    • The Journal of Korean Medicine
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    • v.31 no.1
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    • pp.138-152
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    • 2010
  • Objective: Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) is one of the representative prescriptions for invigoration of vitality and nourishing of the blood. This study was carried out to investigate the effects of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) on hair regrowth and cytokine changes in a shaving model of C57BL6 mice. Method: Five-week-old mice were acclimated for 1 week at a temperature between $21-23^{\circ}C{\acute{E}}$, 40-60% relative humidity, and 12h of a light/dark cycle before beginning of the experiment. There were two groups including normal saline (control) and a positive control of oral intake of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) extract (sample) in 18 female mice. The test compounds were topically treated once a day over 14 days. The hair regrowth was photographically and histologically determined during the experimental period of 14 days. Revelation of TGF-${\beta}1$ and EGF were also determined using immunohistochemistry. In addition to that, IFN-$\gamma$, IL-4 and IL-10 were determined in serum. Results: Hair regrowth in the sample group was promoted earlier and faster than the control group, as shown by concentrations of hairs and thick-hair ratio in the sample group. TGF-${\beta}1$ was not revealed in either control or sample group. EGF was strongly positive in out root sheath of some thick hair of the sample group. Serum IFN-$\gamma$ was significantly decreased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-4 was significantly increased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-10 was decreased in the sample group compared with the control group, but with no real statistical significance. Conclusion: These results suggest that Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) has hair growth promoting activity and it can be used for treatment of alopecia. Also, these effects relate to EGF revelation of hair roots, a decrease in serum IFN-$\gamma$, and an increase of serum IL-4.

Natural 15N Abundances of Corn Treated with Urea and Composted Pig Manure in a Pot Experiment (요소와 돈분퇴비 시용에 따른 포트 재배 옥수수의 질소동위원소 자연존재비 차이)

  • Choi, Woo-Jung;Lee, Sang-Mo;Kim, Kyoung-Cheol;Kim, Pan-Gun;Yoo, Ji-Hyeok;Yoo, Sun-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.4
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    • pp.284-291
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    • 2001
  • To study whether N isotope composition (${\delta}^{15}N$) of crop reflects the kind of fertilizer (chemical or organic) applied to field, a pot experiment was conducted. Corn (Zea mays L.) was cultivated under greenhouse conditions for 70 days. Composted pig manure and urea were applied at 0 and 0 (C0U0), at 0 and 300 (COU2), at 300 and 0 (C2U0) and at 150 and $150kg\;N\;ha^{-1}$ (C1U1), respectively. The ${\delta}^{15}N$ values of composted pig manure and urea were + 13.9‰ and -2.3‰, respectively. The ${\delta}^{15}N$ values of whole parts (roots + stems + leaves + grains) were + 12.7, + 12.9, + 14.0 and + 13.0‰ for C0U0, C0U2, C2U0 and C1U1 treatments, and were not significantly affected by the application of isotopically different N sources (P<0.05). However, leaves or grains showed significantly (P<0.05) different ${\delta}^{15}N$ values between treatments. The ${\delta}^{15}N$ values of leaves and grains were + 14.3 and + 16.2‰ for C2U0, +13.2 and +13.9‰ for C0U0, +10.1 and + 12.6‰ for C1U1 and +10.1 and +12.4‰ for C0U2 treatments. The different ${\delta}^{15}N$ values of corn from the values of N sources (compost and urea) applied to soil showed that the ${\delta}^{15}N$ values of corn were affected not only by the isotope composition of N source, but also by N pool mixing and isotope fractionation accompanying N transformation. This study suggests that although the ${\delta}^{15}N$ values of crop are not identical to the ${\delta}^{15}N$ values of N sources applied to fields, the application of isotopically different N sources such as compost and chemical fertilizer may result in qualitative difference in ${\delta}^{15}N$ values of crop.

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Physicochemical Properties of Waxy Rice Starches Prepared from Three Different Cultivars (다수계 찰벼와 일반찰벼 전분의 이화학적 성질)

  • Kim, Hyong-Soo;Kang, Ock-Joo;Yoon, Gae-Soon
    • Applied Biological Chemistry
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    • v.26 no.4
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    • pp.211-216
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    • 1983
  • The physicochemical properties of starches isolated from three kinds of waxy ricer were investigated. The average diammeter of Orchal starch granule was 3.6u, and those of Hangang and Baegoon starch granules were 4.8u and 5.6u, respectively. X-ray diffraction patterns of all samples were A types. Optical transmittance of starch suspensions was increased rapidly $60{\sim}62^{\circ}C$, $50{\sim}55^{\circ}C$ and $50{\sim}60^{\circ}C$ in Olchal, Hangang and Baegoon, respectively, and all of them exhibited maximum transmittance $75{\sim}80^{\circ}C$ in range. Initial gelationization temperature by means of amylogram pattern was $60{\sim}61^{\circ}C$, then, Olchal starch had a little higher temperature than others. Raising power of them was $260\sim220$, and then, Olchal starch has a little higher raising power. But, water binding capacity of Baegoon and Hangang were somewhat higher than that of Olchal. Blue values of Olchal and Hangang were similar with 0.13, and that of Baegoon was 0. 14. Alkari numbers of Olchal, Hangang and Baegoon were 4.2, 4.9 and 5.1. The degree of retrogradation of Hangang and Baegoon starch gels were somewhat higher than that of Olchal starch gel.

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Oxidation Behavior of Simudated Metallic U-Nb Alloys in Air (모의 금속전환체 U-Nb 합금의 공기중 산화거동)

  • Lee Eun-Pyo;Ju June-Sik;You Gil-Sung;Cho il-Je;Kook Dong-Hak;Kim Ho-Dong
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.2 no.4
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    • pp.239-244
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    • 2004
  • In order to enhance an oxidation resistance of the pure uranium metal under air condition, a small quantity of niobium(Nb) which is known to mitigate metal oxidation is added into uranium metal as an alloying element. A simulated metallic uranium alloy, U-Nb has been fabricated and then oxidized in the range of 200 to $300^{\circ}C$ under the environment of the pure oxygen gas. The oxidized quantity in terms of the weight gain(wt%) has been measured with the help of a thermogravimetric analyzer. The results show that the oxidation resistance of the U-Nb alloy is considerably enhanced in comparison with that of the pure uranium metal. It is revealed that the oxidation resistance of the former with the niobium content of 1, 2, 3, and 4 wt% is : 1) 1.61, 7.78, 11.76 and 20.14 times at the temperature of $200^{\circ}C$ ; 2) 1.45, 5.98, 10.08 and 11.15 times at $250^{\circ}C$ ; and 3) 1.33, 4.82, 8.87 and 6.84 times at $300^{\circ}C$ higher than that of the latter, respectively. Besides, it is shown that the activation energy attributable to the oxidation is 17.13~21.92 kcal/mol.

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