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Effect of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-tang) on Hair Regrowth and Cytokine Changes on Hair-Removed C57BL/6 Mice  

Hong, Jung-Ae (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyunghee University)
Song, Mi-Yeon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyunghee University)
Choi, In-Hwa (Dept. of Oriental Ophthalmology & Otolaryngology & dermatology, College of Oriental Medicine, Kyunghee University)
Sohn, Nak-Won (Dept. of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung-Hee University)
Chung, Seok-Hee (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyunghee University)
Publication Information
The Journal of Korean Medicine / v.31, no.1, 2010 , pp. 138-152 More about this Journal
Abstract
Objective: Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) is one of the representative prescriptions for invigoration of vitality and nourishing of the blood. This study was carried out to investigate the effects of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) on hair regrowth and cytokine changes in a shaving model of C57BL6 mice. Method: Five-week-old mice were acclimated for 1 week at a temperature between $21-23^{\circ}C{\acute{E}}$, 40-60% relative humidity, and 12h of a light/dark cycle before beginning of the experiment. There were two groups including normal saline (control) and a positive control of oral intake of Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) extract (sample) in 18 female mice. The test compounds were topically treated once a day over 14 days. The hair regrowth was photographically and histologically determined during the experimental period of 14 days. Revelation of TGF-${\beta}1$ and EGF were also determined using immunohistochemistry. In addition to that, IFN-$\gamma$, IL-4 and IL-10 were determined in serum. Results: Hair regrowth in the sample group was promoted earlier and faster than the control group, as shown by concentrations of hairs and thick-hair ratio in the sample group. TGF-${\beta}1$ was not revealed in either control or sample group. EGF was strongly positive in out root sheath of some thick hair of the sample group. Serum IFN-$\gamma$ was significantly decreased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-4 was significantly increased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-10 was decreased in the sample group compared with the control group, but with no real statistical significance. Conclusion: These results suggest that Yikgeebohyul-tang (Yiqib$\check{u}$xu$\grave{e}$-t$\bar{a}$ng) has hair growth promoting activity and it can be used for treatment of alopecia. Also, these effects relate to EGF revelation of hair roots, a decrease in serum IFN-$\gamma$, and an increase of serum IL-4.
Keywords
Alopecia; C57BL/6 mice; Yikgeebohyul-tang; hair regrowth;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Neville LF, Mathiak G, Bagasra O. The immunobiology of interferon-gamma inducible protein 10 kD (IP-10): a novel, pleiotropic member of the C-X-C chemokine superfamily. Cytokine Growth Factor Rev. 1997;8(3):207-19.   DOI   ScienceOn
2 Meng X, Sawamura D, Tamai K, Hanada K, Ishida H, Hashimoto I. Keratinocyte gene therapy for systemic diseases. Circulating interleukin-10 released from gene-transferred keratinocytes inhibits contact hypersensitivity at distant areas of the skin. J Clin Invest. 1998;101(6):1462-7.   DOI   ScienceOn
3 Peters EM, Hansen MG, Overall RW, Nakamura M, Pertile P, Klapp BF, et al. Control of human hair growth by neurotrophins: brain-derived neurotrophic factor inhibits hair shaft elongation, induces catagen, and stimulates follicular transforming growth factor beta2 expression. J Invest Dermatol. 2005;124(4):675-85.   DOI   ScienceOn
4 Soma T, Dohrmann CE, Hibino T, Raftery LA. Profile of transforming growth factor-beta responses during the murine hair cycle. J Invest Dermatol. 2003:121(5):969-75.   DOI   ScienceOn
5 Mitsui S, Ohuchi A, Hotta M, Tsuboi R, Ogawa H. Gene for a range of growth factors and cyclin-dependent kinase inhibitors are expressed by isolated human hair follicle. Br. J Dermatol. 1997;137:693.   DOI   ScienceOn
6 Botchkarev VA, Kishimoto J. Molecular control of epithelial-mesenchymal interactions during hair follicle cycling. J Investig Dermatol Symp Proc. 2003;8(1):46-55.
7 Stenn KS, Paus R. Controls of hair follicle cycling. Physiol Rev. 2001;81(1):449-494.
8 Jindo T, Tsuboi R, Imai R, Takamori K, Rubin JS, Ogawa H. The effect of hepatocyte growth factor/scatter factor on human hair follicle growth. J Dermatol Sci. 1995;10(3):229-32.   DOI   ScienceOn
9 Mak KK, Chan SY. Epidermal growth factor as a biologic switch in hair growth cycle. J Biol Chem. 2003;278(28):26120-6.   DOI   ScienceOn
10 Inui S, Fukuzato Y, Nakajima T, Yoshikawa K, Itami S. Androgen-inducible TGF-beta1 from balding dermal papilla cells inhibits epithelial cell growth: a clue to understand paradoxical effects of androgen on human hair growth.FASEB J. 2002;16(14):1967-9.
11 Lee hs, Song JY, Yun Km, Jae YM. Hair growth effect of Mori Cortex Mixture on the hair of rat. Korean J. Seric. Sci. 2000;42(2):83-5.
12 Choi W, Choi JH, Kim JH. Effect on Hair Growth by various herb medicine. The Journal of Oriental Ophthalmology & Otolaryngology & dermatology. 2002;15(2):80-103.
13 Shin YS. Studies on the effect of five herbal extracts on hair growth activity. Dept. of Oriental MedicineGraduate School Daejeon University. 2003.
14 Kim MY, An BJ. A Study of Hair Loss Prevention and Hair Growth Promotion by Korean Traditional Fomular. Korean journal of herbology. 2004:19 (2);9-20.   과학기술학회마을
15 Park WS, Sung DS, Kim DK, Cho WH, Lee HK, Lee CH, et al. The Effect of Hair Essence (HHRHG0202-80) Containing Five Herbal Extracts on Hair Growth and the Prevention of Alopecia in vitro& vivo. J Korean Oriental Med. 2004;25 (1):152-60.   과학기술학회마을
16 Kim JH, Lee KS. Experimental Study on the Effects of Jaeumgeonbi-tang on Sedative Action and Hematosis. J Korean Oriental Med. 1988;9 (1):35-41.
17 Lee HY, Baek TH. Effect of Jaeumgeonbi-tang Extract on Indomethacin-Induced Gastric Mucosal Lesions. J Korean Oriental Med. 2004;25(3):111-22.   과학기술학회마을
18 Lim KM, Jeong HW. Mechanism of Jaeumgeonbitang adding Aurantii Fructus.Gastrogae Rhizoma on the Improvement and Changes of Cerabral Hemodynamics. Korean Journal of Oriental Physiology and Pathology. 2003;17(1):64-70.   과학기술학회마을
19 Hong DS. The Effect of Jaeumgunbitang on The Brain Catecholamine Contents and Body Weight of Rats in Immobilization Stress. Graduate School Kyung Hee University. 1992.
20 Randall VA, Sundberg JP, Philpott MP. Animal and in vitro models for the study of hair follicles. J Investig Dermatol Symp Proc. 2003;8(1):39-45.   DOI   ScienceOn
21 Powell B, Crocker L, Rogers G. Hair follicle differentiation: expression, structure and evolutionary conservation of the hair type II keratin intermediate filament gene family. Development. 1992;114(2):417-33.
22 Rogers GE. Hair follicle differentiation and regulation. Int J Dev Biol. 2004;48(2-3):163-70.   DOI   ScienceOn
23 Kreplak L, Merigoux C, Briki F, Flot D, Doucet J. Investigation of human hair cuticle structure by microdiffraction: direct observation of cell membrane complex swelling. Biochim Biophys Acta. 2001;1547(2):268-74.   DOI   ScienceOn
24 Soma T, Ogo M, Suzuki J, Takahasi T, Hibino T. Analysis of apoptotic cell death in human hair follicles. J Invest Dermatol 1998;11:948-954.
25 Kalish RS, Gilhar A. Alopecia areata: autoimmunitythe evidence is compelling. J Invest Dermatol Symp Proc. 2003;8:164-167.   DOI   ScienceOn
26 Paus R, Hofmann U, Eichmuller S, Czarnetzki BM. Distribution and changing density of gamma-delta T cells in murine skin during the induced hair cycle. Br J Dermatol. 1994;130(3): 281-9.   DOI   ScienceOn
27 Peus D, Pittelkow MR. Growth factors in hair organ development and the hair growth cycle. Dermatol Clin. 1996;14(4):559-72.   DOI
28 Paus R, Christoph T, Muller-Rover S. Immunology of the hair follicle: a short journey into terra incognita. J Invest Dermatol Symp Proc. 1999;4 (3):226-34.   DOI   ScienceOn
29 Christoph T, Muller-Rover S, Audring H, Tobin DJ, Hermes B, Cotsarelis G, et al. The human hair follicle immune system: cellular composition and immune privilege. Br J Dermatol. 2000;142 (5):862-73.   DOI   ScienceOn
30 Ito T, Ito N, Saathoff M, Bettermann A, Takigawa M, Paus R. Interferon-gamma is a potent inducer of catagen-like changes in cultured human anagen hair follicles. Br J Dermatol. 2005;152 (4):623-31.   DOI   ScienceOn
31 Bodemer C, Peuchmaur M, Fraitaig S, Chatenoud L, Brousse N, De Prost Y. Role of cytotoxic T cells in chronic alopecia areata. J Invest Dermatol. 2000;114(1):112-6.   DOI   ScienceOn
32 Miceli MC, Parnes JR. Role of CD4 and CD8 in T cell activation and differentiation. Adv Immunol. 1993;53:59-122.   DOI
33 Kwon GJ . Experimental Studies on Hair Growth Effect of Samultang-gamibang in Spontaneous Alopecia Areata Mice. Professional Graduate School of Oriental Medicine Wonkwang University.2004.
34 KIm PS, Kim HT, Roh SS, Whang CY. Effect of DanGuiBoHyulTangGami-Bang on the Alopecia and Hair Growth Stimulation. The Journal of Oriental Ophthalmology & Otolaryngology & dermatology. 2004;17(3):38-60.
35 Kim JJ . Dongyangeuihak Jinryoyogam. Seoul: The Institute of Oriental medicine. 2007;176.
36 Park SD, Seo GS, Park WH. Effects of Bojungikgitang, Soeumin Bojungikgi-tang and its component groups on Antioxidant Activities in alloxaninduced diadetic rats. Kor. J. Herbology. 2001;16 (2):113-26.
37 Lee G. Dongyuashizhongyishu. Seoul:Daeseong medicine publishing company. 1983;35-7,86-7.
38 Youn GY. Dongeuiyimsangbangjehak. Seoul: Myungbo publishing company. 1987;254-63,303-8.
39 Baek TH, Jee HC. Study on effect of Bojungikgitang affecting lactate tolerance and recovery rate. Journal of Oriental Rehabilitation Medicine. 1998;8(1):171-86.
40 Kim JS, Sim SW, U YS, Nam HJ, Kim KJ. The Effect of Bojungikgi-tang on the NAsal tissue in allergic rhinitis of ovalbimin-inhalation Rats. Journal of the oriental medical surgery, ophthalmology & otolaryngology. 2004;17(1):66-74.
41 Eun JS, Choi H, Song JM. Effect of Bojungikgitang on the Specific immune Response in BALB/c Mice. Korean J. Oriental Physiology & Pathology. 2002;16(5):943-7.   과학기술학회마을
42 Gong TX. Wanbinghuichun. Seoul:Haenglim publishing company. 1972;220-2.
43 Heo J. Dongeuibogam. Seoul:Namsandang. 1983 ;202-3,359.
44 Chen SW. Taipinghuiminhejijufang. Beijing:People's Medical Publishing House. 1985;179-80, 310-1.
45 Yang YG. Huangdineijingsuwenyijie. Seoul:Seongbosa 1980;5-10,93-4,337,340.
46 Yang YG. Huangdineijinglingshuyikjie. Seoul: Seongbosa. 1980;104,452-6.
47 Hirobe T. Structure and function of melanocytes: microscopic morphology and cell biology of mouse melanocytes in the epidermis and hair follicle. Histol Histopathol. 1995;10(1):223-37.
48 Li J, Tzu J, Chen Y, Zhang YP, Nguyen NT, Gao J, et al. Laminin-10 is crucial for hair morphogenesis. EMBO J. 2003;22(10):2400-10.   DOI   ScienceOn
49 Lindner G, Botchkarev VA, Botchkareva NV, Ling G, van der Veen C, Paus R. Analysis of apoptosis during hair follicle regression (catagen). Am J Pathol. 1997;151(6):1601-17.
50 Danilenko DM, Ring BD, Yanagihara D, Benson W, Wiemann B, Starnes CO, et al. Keratinocyte growth factor is an important endogenous mediator of hair follicle growth, development, and differentiation. Normalization of the nu/nu follicular differentiation defect and amelioration of chemotherapy-induced alopecia Am J Pathol. 1995;147(1):145-54.
51 Chao YF. Zhubingyuanhoulun. Beijing:People's Medical Publishing House. 1983;761-7.
52 Lim SBN, Choi KD, Kim SK. The Oriental and Western Medical Study of Alopecia. The Journal of Jeahan Oruental Medical Academy. 1999;4(1):699-710.
53 Gilhar A, Landau M, Assy B, Ullmann Y, Shalaginov R, Serafimovich S, et al. Transfer of alopecia areata in the human scalp graft/ Prkdc(scid) (SCID) mouse system is characterized by a TH1 response. Clin Immunol. 2003;106(3):181-7.   DOI   ScienceOn
54 Otomo S. new hair growth drugs and their nonclinical evaluation methods. Hyangjanghwoiji. 1997;21:228.
55 Tanyasiri K, Hira K, Mitsuishi K, Ueki R, Sekigawa I, Ogawa H. Interleukin-16 in patients with alopecia areata. J Dermatol Sci. 2005;37 (1):55-7.   DOI   ScienceOn
56 Kim JH, Lee TH. Effects of Bojungikgitang on Starvation stress in Mice. The Korean Journal of Oriental Medical Prescription. 2006;14(1):133-40.
57 Kim SM, Sim SY, Byun HS, Kim KJ. The Effect of Bojungikgi-tang in mouse model of Allergic rhinitis. The Journal of Oriental Ophthalmology & Otolaryngology & dermatology. 2005;18(3):26-36.
58 Kagoura M, Morohashi M, Ogita Z. Animal models for the study of alopecia. Hyangjanghwoiji. 1997;21:207.
59 Hwang HJ, Kim JD. A Case Study on the Iron Deficiency Anemia. Korean J. Orient. Int. Med. 2000;21(4):655-60.