• Title/Summary/Keyword: "The cat"

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Nurses' Attitudes Toward Complementary and Alternative Therapies (간호사의 보완대체요법에 대한 태도)

  • Son, Haeng-Mi
    • Korean Journal of Adult Nursing
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    • v.14 no.1
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    • pp.62-72
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    • 2002
  • Purpose: This study was performed to develop a scale for evaluation of attitudes toward complementary and alternative therapies (CAT) and to investigate nurses' attitudes toward CAT. Method: The subjects were 263 nurses working at a university hospital in Seoul and Inchon. The personally designed questionnaire was tested for its reliability and validity. Nurses' attitudes to CAT were evaluated using the questionnaire. Results: Cronbach's $\alpha$ coefficient was 0.7405. 23 items were selected by item analysis and 4 factors including application, therapeutic effect, social interest and communication about CAT were classified by factor analysis. The mean score of attitudes and its subcategories were high, especially that of communication was very high. Nurses had a positive response to CAT in several items; acceptance as nursing intervention, its therapeutic value, complement for conventional medicine, and open communication about CAT. Attitudes were different significantly according to education and number of working years. There were high relationships between attitudes and its subcategories except communication. Conclusion: The Scale of attitudes toward CAT was proven to be reliable and valid. Positive nurses' attitudes toward CAT will help the patients be provided with a proper and safe way to take CAT.

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Simultaneous and Sequential Co-Immobilization of Glucose Oxidase and Catalase onto Florisil

  • Gul, Ozyilmaz;Tukel, S. Seyhan
    • Journal of Microbiology and Biotechnology
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    • v.17 no.6
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    • pp.960-967
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    • 2007
  • The co-immobilization of Aspergillus niger glucose oxidase (GOD) with bovine liver catalase (CAT) onto florisil (magnesium silicate-based porous carrier) was investigated to improve the catalytic efficiency of GOD against $H_2O2$ inactivation. The effect of the amount of bound CAT on the GOD activity was also studied for 12 different initial combinations of GOD and CAT, using simultaneous and sequential coupling. The sequentially co-immobilized GOD-CAT showed a higher efficiency than the simultaneously co-immobilized GOD-CAT in terms of the GOD activity and economic costs. The highest activity was shown by the sequentially co-immobilized GOD-CAT when the initial amounts of GOD and CAT were 10 mg and 5 mg per gram of carrier. The optimum pH, buffer concentration, and temperature for GOD activity for the same co-immobilized GOD-CAT sample were then determined as pH 6.5, 50 mM, and $30^{\circ}C$, respectively. When compared with the individually immobilized GOD, the catalytic activity of the co-immobilized GOD-CAT was 70% higher, plus the reusability was more than two-fold. The storage stability of the co-immobilized GOD-CAT was also found to be higher than that of the free form at both $5^{\circ}C\;and\;25^{\circ}C$. The increased GOD activity and reusability resulting from the co-immobilization process may have been due to CAT protecting GOD from inactivation by $H_2O2$ and supplying additional $O_2$ to the reaction system.

Analysis of the Dual Promoters and the $H_2O$$_2$-responsive Element of the cats Gene Encoding Catalase A in Streptomyces coelicolor

  • Cho, You-Hee;Hahn, Ji-Sook;Roe, Jung-Hye
    • Journal of Microbiology
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    • v.38 no.4
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    • pp.239-244
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    • 2000
  • The cats gene encodes the major catalase in Sreptomyces coelicolor, whose production increases upon H$_2$O$_2$treatment. Besides the previously identified primary promoter (catApl), a minor promoter (catAp2) was newly assigned by S1 nuclease mapping. The catAp2 transcript was observed transiently upon entry into the stationary phase in liquid culture and upon differentiation on solid plates, whereas the level of catApl transcription did not chance significantly during this growth transition. ThecatApl promoter was transcribed by the major vegetative RNA polymerase holoenzyme containing $\sigma$$\^$HrdB/, whereas the catAp2 was transcribed in vitro by the holoenzyme containing $\sigma$$\^$R/ that is activated under oxidative conditions. The cia-element regulating the H$_2$O$_2$-inducibility of catApl was identified within the 23 bp inverted repeat sequence located between -65 and -43 of the catApl promoter. We roamed this sequence HRE (H$_2$O$_2$-responsive Element). The distal half of the inverted repeat was more crucial for H$_2$O$_2$-dependent induction of the catApl transcript than the proximal half. HRE most likely serves as a binding site for the H$_2$O$_2$-responsive repressor CatR.

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Spinal Lymphoma in a Cat (고양이에서 척수림프종의 발생례)

  • 권오경;홍성혁
    • Journal of Veterinary Clinics
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    • v.17 no.1
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    • pp.279-284
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    • 2000
  • A 3.5kg, 3-year-old castrated male Japanese domestic cat withpelvic limb ataxia and dysuria was referred to the Veterinary Medical Center of the Tokyo University. On the neurologic examination findings, both pelivic had a LMN paresis. The cat was FeLV positive and FIV negative. Radiographic findings did not identify the spinal lesions. In magnetic resonance images(MRI) of the lumbar spinal cord, the cat had the lesions in the lumbar(L) 1 and L3, characterized by hyperintensity on a transverse T2-weighted and T1-weighted images, and contrast enhancement was evident. The mass removed by dorsal laminectomy. Histopathological examination of the mass revealed spinal lymphoma. Three weeks after the surgery, the cat administered chemotherapy protocol for lymphoma by current protocol. Two weeks after chemotherapy, the cat had a metastasis to bone marrow and died.

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Evaluation of EtherCAT Clock Synchronization in Distributed Control Systems (분산 제어 시스템을 위한 EtherCAT 시계 동기화의 성능 평가)

  • Kim, Woonggy;Sung, Minyoung
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.38 no.7
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    • pp.785-797
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    • 2014
  • Support for the precise time synchronization of EtherCAT, known as distributed clock (DC), enables the design of highly synchronized operations in distributed real-time systems. This study evaluates the performance of the EtherCAT DC through extensive experiments in a real automation system. We constructed an EtherCAT control system using Xenomai and IgH EtherCAT stack, and analyzed the clock deviation for different devices in the network. The results of the evaluation revealed that the accuracy of the synchronized clock is affected by several factors such as the number of slave devices, period of drift compensation, and type of system time base. In particular, we found that careful decision regarding the system time base is required because it has a fundamental effect on the master operation, which results in significantly different performance characteristics.

ROLE OF 5'FLANKING DNA FOR THE REGULATION OF CYP450IA1 GENE EXPRESSION

  • Sheen, Yhun-Y.
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1995.10a
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    • pp.153-155
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    • 1995
  • To investigate the mechanism of the regulation of cytochrome P450IAl, the 5'-flanking region of a trout cytochrome P4501Al was cloned into the CAT basic expression vector at HindⅢ site. This trout Cytochrome P450IAl upstream DNA containing CAT construct was transfected into Hepa-1 cells .3MC treatment to hepa I cells transfected with trout P450IAl-CAT construct increased CAT protein and mRNA by 2.81 fold when it was compared with that of control. This increase CAT protein and mRNA was decreased by concomitantly treated flavonoids and aminopyrine. The level of CAT protein was 29.2-58.0% of 3MC stimulated CAT protein.

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Performance Evaluation of a Real-time EtherCAT Master According to Network Controllers (실시간 EtherCAT 마스터의 네트워크 컨트롤러에 따른 성능 평가)

  • Hwa Il Seo;Sung Jin Kang
    • Journal of the Semiconductor & Display Technology
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    • v.23 no.2
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    • pp.19-22
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    • 2024
  • EtherCAT is an Ethernet-based fieldbus system standardized in IEC 61158 and SEMI, and widely used in the fields of factory automation, semiconductor equipment and robotics. In this paper, we summarize the current status of Xenomai real-time framework and RTnet, which are essential for Linux operating systems to operate in real-time, and implement a real-time EtherCAT master system with these open sources. The real-time performance of the implemented EtherCAT master is evaluated according to Intel network controllers 82574L, I219, I210, and I225, respectively. The results show that the implemented EtherCAT master provides precise control performance for control frequencies from 1KHz to 8KHz and similar performance for I219, I210, and I225, and relatively slightly larger jitter for 82574L.

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Factors Influencing on Use of Complementary.Alternative Therapy on Chronic Patients (만성질환자의 보완대체요법 이용에 영향을 미치는 요인)

  • Park, Hyeon-Shin;Hyun, Kyung-Sun
    • Journal of East-West Nursing Research
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    • v.17 no.1
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    • pp.1-8
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    • 2011
  • Purpose: The purpose of this study was to identify influencing factors of using numbers and period of complementary and alternative therapy (CAT) among chronic patients. Methods: The study employed a descriptive design. The participants were 221 chronic patients. Data were collected in a pharmacy near a general hospital from April to July, 2008. Results: Influencing factors on using number of CAT were age, numbers of disease, period of used CAT and monthly expenses on CAT (the ability to explain, 79.9%). Influencing factors on using period of CAT were the period of illness and using number of CAT (the ability to explain, 44.9%). Conclusion: The results of this study suggest that chronic patients need counseling and guides about using CAT especially for those are older and have many kinds and longer period of diseases.

Studies on catalase type in Cheju native horse (제주마(濟州馬)의 catalase형(型)에 관(關)한 연구(硏究))

  • Hyun, Hae-sung;Kim, Woo-kwon;Chang, Duk-gi
    • Korean Journal of Veterinary Research
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    • v.31 no.2
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    • pp.167-170
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    • 1991
  • The catalase phenotypes and the gene frequencies in erythrocyte of 223 Cheju native horses were studied by starch gel electrophoresis. The results obtained were as follows: 1. In the catalase phenotypes, three phenotypes, CatF, CatM and CatS, which were controlled by two allelic genes, $Cat^F$ and $Cat^S$, were observed and their frequencies of appearance were 24.21%, 47.53%, and 28.25% respectively. 2. The distribution of gene frequency was calculated as 0.480 in $Cat^F$ and 0.520 in $Cat^S$.

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In Vivo Quantitative Analysis of PKA Subunit Interaction and cAMP Level by Dual Color Fluorescence Cross Correlation Spectroscopy

  • Park, Hyungju;Pack, Changi;Kinjo, Masataka;Kaang, Bong-Kiun
    • Molecules and Cells
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    • v.26 no.1
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    • pp.87-92
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    • 2008
  • We employed dual color Fluorescence Cross Correlation Spectroscopy (FCCS) to measure the interaction between PKA regulatory (RII) and catalytic subunits (CAT) in living cells. Elevation of intracellular cAMP with forskolin decreased the cross-correlation amplitude between RFP-fused RII (RII -mRFP) and GFP-fused CAT (CAT-EGFP) by 50%, indicating that cAMP elevation leads to dissociation of RII-CAT complexes. Moreover, diffusion coefficient analysis showed that the diffusion rate of CAT-EGFP was significantly increased, suggesting that the decreased RII-CAT association caused by cAMP generated free CAT subunits. Our study demonstrates that in vivo FCCS measurements and their quantitative analysis permit one not only to directly quantify protein-protein interactions but also to estimate changes in the intracellular cAMP concentration.