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http://dx.doi.org/10.7318/KJFC/2021.36.2.235

Anti-proliferative and Apoptotic Activity of Extracts of Lindera glauca Blume root in Human HCT116 Colorectal Cancer Cells  

Kim, Yeah-Un (Department of Food and Nutrition, Kwangju Women's University)
Moon, Ha-Rin (Department of Food and Nutrition, Chonnam National University)
Han, Inhwa (Department of Food and Nutrition, Kwangju Women's University)
Yun, Jung-Mi (Department of Food and Nutrition, Chonnam National University)
Publication Information
Journal of the Korean Society of Food Culture / v.36, no.2, 2021 , pp. 235-245 More about this Journal
Abstract
Lindera glauca Blume has been used in Korean traditional medicine to treat the symptoms of paralysis, abdominal pain, speech disorders, extravasations, contusions, and pain caused by rheumatoid arthritis. We investigated the effect of L. glauca Blume extracts on the proliferation of colorectal cancer cells in vitro using HCT116 human colorectal cancer cell lines. We also investigated its mechanism of action. For this purpose, we used the MTT assay, western blotting, DNA fragmentation analysis, and flow cytometry. HCT116 cells were cultured in several concentrations of ethanol extracts of L. glauca Blume root (0, 50, 100 ㎍/mL). In this study, colon cancer cell growth was inhibited by L. glauca Blume root extract in a dose-dependent manner. It was associated with induction of apoptosis as assessed by nuclear fragmentation and cell cycle analysis. Apoptosis was assessed using western blotting for TNF-α, IL-6, NF-κB, Caspase-3, PARP, Bax, Bcl-2, and SIRT1. The extract also dose-dependently upregulated the expression Bax, the pro-apoptotic gene and downregulated the expression of the anti-apoptotic gene Bcl-2. Furthermore, the extract enhanced Caspase-3 activity in a dose-dependent manner. Our findings provide evidence that L. glauca Blume extract may mediate its anti-proliferative effect via the modulation of apoptosis.
Keywords
Lindera glauca Blume; anti-proliferative activity; apoptosis; HCT116; colorectal cancer cells;
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