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http://dx.doi.org/10.13050/foodengprog.2013.17.3.271

Development of Rapid Detection Kit for Aflatoxin B1 in Cereals  

Choi, Sunmi (Department of Food and Nutrition, Hanyang University)
Kim, Giyoung (Department of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration)
Lee, Sangdae (Department of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration)
Moon, Jihea (Department of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration)
Om, Aeson (Department of Food and Nutrition, Hanyang University)
Publication Information
Food Engineering Progress / v.17, no.3, 2013 , pp. 271-275 More about this Journal
Abstract
The aim of this study is to develop the one dot rapid detection kit that meets the limit of detection (LOD) of KFDA (10 ${\mu}g/kg$) for Aflatoxin $B_1$ (AFB1). The fabrication process of the one dot rapid detection kit, such as gold nanoparticle and AFB1-BSA conjugation, and AFB1-polyclonal antibody concentration was optimized. Fabricated rapid detection kits were tested with the standard AFB1 solutions and the solutions extracted from contaminated maize samples. The LOD in the standard AFB1 solution and extracted AFB1 solution was 5 ${\mu}g/kg$ for both. This result is lower than the LOD of KFDA in food (10 ${\mu}g/kg$) and satisfies the LOD of EU (2-12 ${\mu}g/kg$) and Japan (10 ${\mu}g/kg$) in food.
Keywords
Aflatoxin B1; Rapid detection kit; Cereals; Corn;
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Times Cited By KSCI : 3  (Citation Analysis)
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