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http://dx.doi.org/10.11002/kjfp.2014.21.3.442

Characterization of alkaline cellulase from Bacillus subtilis 4-1 isolated from Korean traditional soybean paste  

Baek, Seong Yeol (Fermented Food Division, Department of Agro-food Resource, NAAS, RDA)
Lee, You Jung (Fermented Food Division, Department of Agro-food Resource, NAAS, RDA)
Yun, Hye Ju (Fermented Food Division, Department of Agro-food Resource, NAAS, RDA)
Park, Hye Young (Fermented Food Division, Department of Agro-food Resource, NAAS, RDA)
Yeo, Soo-Hwan (Fermented Food Division, Department of Agro-food Resource, NAAS, RDA)
Publication Information
Food Science and Preservation / v.21, no.3, 2014 , pp. 442-450 More about this Journal
Abstract
In this study, we isolated a cellulase-producing bacterium isolated from traditional Korean fermented soybean paste and investigated the effect of culture conditions on the production of cellulase. This bacterium, which was identified as Bacillus subtilis 4-1 through 16S rRNA gene sequence analysis, showed the highest cellulase activity when the cells were grown at $45^{\circ}C$ for 24 hours in the CMC medium supplemented with 1.0% of soluble starch and 0.1% yeast extract. The initial optimum pH of the medium was observed in the range of 5.0~9.0. The optimal pH and temperature for the production of cellulase from B. subtilis 4-1 were pH 9.0 and $60^{\circ}C$ respectively. In addition, the enzyme showed significant activity in the temperature range of $20{\sim}90^{\circ}C$, which indicates that B. subtilis 4-1 cellulase is an alkaline-resistance and thermo-stable enzyme. This enzyme showed higher activity with CMC as the substrate for endo-type cellulase than avicel or pNPG as the exo-type substrates for exo-type cellulase and ${\beta}$-glucosidase. These results suggest that the cellulase produced from B. subtilis 4-1 is a complex enzyme rather than a mono-enzyme.
Keywords
cellulase; Bacillus sp. cellulase; soybean paste; fermented foods;
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Times Cited By KSCI : 8  (Citation Analysis)
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