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http://dx.doi.org/10.11002/kjfp.2011.18.3.358

Neuronal Cell Protection and Antioxidant Activities of Hot Water Extract from Commercial Buckwheat Tea  

Jeong, Chang-Ho (Department of Food Science and Biotechnology, Institute of Life Science an Resources, Kyung Hee University)
Jeong, Hee-Rok (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
Choi, Sung-Gil (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
Shim, Ki-Hwan (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
Heo, Ho-Jin (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Food Science and Preservation / v.18, no.3, 2011 , pp. 358-365 More about this Journal
Abstract
The antioxidant and neuronal cell-protective effects of hot water extract from commercial buckwheat tea (CBTE) were evaluated. The 2,2'-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and malondialdehyde (MDA) inhibitory effect of the CBTE increased in a dose-dependent manner. The Intracellular reactive oxygen species (ROS) accumulation that resulted from hydrogen peroxide ($H_2O_2$) treatment more significantly decreased when CBTE was present in the media than when the PC12 cells were treated only with $H_2O_2$. In the neuronal cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT), the aqueous extracts showed a protective effect against $H_2O_2$-induced neurotoxicity, and the lactate dehydrogenase (LDH) release into the medium was also inhibited by CBTE. The total phenolics of CBTE was 9,608.10 mg/100 g, and the major phenolic compounds were rutin (13.42 mg/100 g) and quercitrin (0.90 mg/100 g). These data suggested that CBTE, including the aforementioned phenolics, may be useful in reducing the risk of neurodegenerative disease.
Keywords
Commercial buckwheat tea; rutin; antioxidant; neuronal cell protection;
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