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Protective Effects of Helianthus annuus Seed Extract against Chemical-Induced Neuronal Cell Death  

Park, Ja-Young (Department of Food Science & Technology, Kyungpook National University)
Woo, Sang-Uk (Department of Food Science & Technology, Kyungpook National University)
Heo, Jin-Chul (Food and Bio-Industry Research Institute, Kyungpook National University)
Lee, Sang-Han (Department of Food Science & Technology, Kyungpook National University)
Publication Information
Food Science and Preservation / v.14, no.2, 2007 , pp. 213-219 More about this Journal
Abstract
To develop an anti-dementia agent with potential therapeutic value in the protection of neuronal cells, we selected a water extract of Helianthus annuus seed for analysis. We measured acetylcholinesterase inhibitory activity in the extract, and analyzed the protective effect of the extract on neuronal cell death induced by hydrogen peroxide, or amyloid ${\beta}-peptide$, of SH-SY5Y neuroblastoma cells. The result showed that the extinct exerted protective effects of 83%, 72% and 53% respectively, on cell death induced by 100M, 200M, and 500M hydrogen peroxide. Also, when 50M of amyloid ${\beta}-peptide$ was added to the cells, the extract showed a protective effect (up to 80%) on cell death. Overall, the results showed that the H. annuus extract inhibited acetylcholinesterase activity in a dose-dependent manner, and the extract also strongly protected against cell death induced by hydrogen peroxide or amyloid ${\beta}-peptide$.
Keywords
Amyloid ${\beta}-peptide$; Hydrogen peroxide; Neuronal cell death; Helianthus annuus seed extract;
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