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Screening of Fibrinolytic Enzyme Producing from Microorganisms in Korean Fermented Soybean Paste and Optimum Conditions of Enzyme Production.  

Ok Min (Department of Functional Food Consulting, Sorabol college)
Choi Young-Su (Department of Biotechnology, Dong-A University)
Publication Information
Food Science and Preservation / v.12, no.6, 2005 , pp. 643-649 More about this Journal
Abstract
This study was investigated to find out optimal medium maximizing the production of fibrinolytic enzyme by Bacillus sp. isolated from Korean fermented soybean paste, which could hydrolyze the fibrin produced through the blood coagulation mechanism in human body. Among carbon sources tested, galactose was most effective for the enzyme production, and the level of the concentration for the optimal enzyme production was $4\%$(w/v). For nitrogen sources tested, malt extract was most effective for the enzyme production, and level of the concentration for optimal enzyme production was $4\%$(w/v). For mineral sources tested, $K_2HPO_4$ was most effective for enzyme production. The enzyme was maximally produced by cultivating the organism at the liquid medium of the initial pH 6 and temperature of $40^{\circ}C$.
Keywords
Bacillus; fibrinolytic enzyme; korean fermented soybean paste;
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