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Antimutagenic and Cytotoxic Effects of Acer ginnala Max. Bark Extracts  

Oh Heung-Seok (Gangwon Institute of Health and Environment)
Cui Cheng-Bi (Department of Food Science and Engineering, Agricultural college of Yanbian University)
Choi Hyung-Taek (Shin Won Food Industry Co., LTD.)
Kim Soo-Hyun (School of Biotechnology and Bioengineering, Kangwon National University)
Jeon Mi-Sun (School of Biotechnology and Bioengineering, Kangwon National University)
Ham Seung-Shi (School of Biotechnology and Bioengineering, Kangwon National University)
Publication Information
Food Science and Preservation / v.11, no.4, 2004 , pp. 550-556 More about this Journal
Abstract
In the present study, we investigated the antimutagenic and cytotoxic effects of Acer ginnala Max. bark extract on S. typhimurium TA98, TA100 and cancer cell lines with Ames test and SRB assay, respectively. They were extracted with methanol and then fractionated using hexane, chloroform, ethyl acetate, butanol, and water to obtain the fractions. The inhibition rate of methanol ($200\;{\mu}g/plate$) of Acer ginnala Max. bark extract in the Salmonella typhimurium TA100 strain showed $83.3\%$ against the mutagenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). In addition, the suppression of methanol extract with same concentration of in the Salmonella typhimurium TA98 and TA100 strains showed $80.3\%\;and\;92.7\%$ inhibition against 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indol (Trp-P-1), respectively. The cytotoxicity effects of Acer ginnala Max. bark extract against the cell lines with human lung carcinoma (A549), human gastric carcinoma (AGS), human hepatocellular carcinoma (Hep3B) and human breast adenocarcinoma (MCF-7) were inhibited with the increase of the extract concentration. The treatment of 1.0 mg/mL Acer ginnala Max. bark methanol extract of methanol showed strong cytotoxicities of $77.3\%,\;90.4\%,\;88.9\%,\;and\;83.7\%$ against A549, AGS, Hep3B and MCF-7, respectively.
Keywords
Acer ginnala Max.; antimutagenicity; cytotoxicity;
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