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Inhibitory Effect of Grapefruit Seed Extract Mixture on the Physiological Function of Botrytis cinerea  

Cho Sung-Hwan (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
Kim Chul-Hwan (Department of Forest Products, Gyeongsang National University)
Park Woo-Po (Division of Food Science, Masan College)
Publication Information
Food Science and Preservation / v.11, no.3, 2004 , pp. 417-423 More about this Journal
Abstract
Botanical antimicrobial agent-grapefruit seed extract mixture (BAAG) have an unknown compounds which exhibit the antibiotic activities aganist microorganisms including bacteria and fungi. We have examined the effects of BAAG on the physiological function of Botrytis cinerea which was isolated from necrotic lesions of decayed fruits and vegetables such as cucumbers, grapes, tomatoes, and red peppers during storage. In the results of enzymatic activities related to the energetic metabolism there was no inhibitory effect of BAAG on the activities of several enzymes in vitro including glucose 6-phosphate dehydrogenase and malate dehydrogenase, while there was inhibitory effect of BAAG on the activities of hexokinase and succinate dehydrogenase. O-nitrophenyl-$\beta$-D-galactopyranoside(ONPG), the artificial substrate of $\beta$-galactosidase was hydrolyzed in the presence of BAAG, indicating that the membrane was pertubated by the BAAG. From the results we suggested that the antibiotic activity of BAAG is due to the change of membrane permeability of the cell. BAAG was fractionated and purified by silica gel and sephadex column chromatography. Among active fractions two peaks were identified as naringin and limonin when they were analyzed by by NMR and Fast atomic bombardment.
Keywords
botanical antimicrobial agent-grapefruit seed extract mixture; energetic metabolism; beta-galactosidase; fast atomic bombardment;
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