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Effects of n-Hexane Fraction of Angelica acutiloba on Antioxidative System and Lipid peroxidation in Ethanol-Induced Hepatotoxicity of rats  

Choo Myung-Hi (Dept. of Food and Nutrition, Chosun University)
Choi Hyun-Suk (Dept. of Food and Nutrition, Chosun University)
Seo Young-Nam (Dept. of Food and Nutrition, Chosun University)
Lee Myung-Yul (Dept. of Food and Nutrition, Chosun University)
Publication Information
Food Science and Preservation / v.11, no.3, 2004 , pp. 364-372 More about this Journal
Abstract
To investigate antioxidative effects of n-hexane fraction of Angelica acutiloba on the ethanol-induced hepatotoxicity of rats, Sprague-Dawley rats weighing 100 $\pm$ 20 g were divided into 5 groups; normal group(NOR), ethanol(10 mL/kg, 35$\%$) treated group(CON), n-hexane fraction of Angelica acutiloba 70 mg/kg treated group(Al), n-hexane fraction of Angelica acutiloba 70 mg/kg and ethanol treated group(A2) and n-hexane fraction of Angelica acutiloba 140 mg/kg and ethanol treated group(A3), respectively. The antioxidative activities of ethanol extract of Angelica acutiloba in vitro were decreased in order of n-hexane > ethylacetate > chlorofonn > n-butanol (>) water fraction. The growth rate and feed efficiency rate decreased by ethanol were gradually increased to the adjacent level of the normal group by administering n-hexane fraction of Angelica acutiloba. It was also observed that the activities of SOD of liver, ALT and AST of serum increased by ethanol were markedly decreased in n-hexane fraction of Angelica acutiloba administered group, and not in activites of XO, catalase, as compared with the control group. The depleted content of GSH by ethanol was increased adjacent to normal level by administering n-hexane fraction of Angelica acutiloba. as a dose-dependent manner. These results suggested that n-hexane fraction of Angelica acutiloba has a possible protective effect on the ethanol-induced hepatotoxicity of rats.
Keywords
protective effect; hepatotoxicity; TBA-reactants; antioxidative activities;
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