Browse > Article

Biological Activities of Ethanol Extracts and Fractions of Black Olympia Grape(Vitis Labruscana L.)  

박성진 (강원대학교 바이오산업공학부)
박부길 (강원대학교 바이오산업공학부)
이현용 (강원대학교 바이오산업공학부)
오덕환 (강원대학교 바이오산업공학부)
Publication Information
Food Science and Preservation / v.9, no.3, 2002 , pp. 338-344 More about this Journal
Abstract
This study was conducted to determine biological activities, such as lipid peroxidation inhibition and cytotoxic effect of ethanol extracts of Black Olympia grape seeds and skins, and of organic solvent fractionated ethanol extracts obtained from grape seeds and skins at different temperatures. Among different extraction temperatures, the ethanol extract of grape seed obtained at 30$\^{C}$ had the strongest lipid oxidation inhibition of 60.1%, while the strongest lipid oxidation inhibitory effect of 71.2% was observed in the presence of 20 $\mu\textrm{g}$/㎖ ethylacetate fraction obtained from ethanol extract of grape seeds at 30$\^{C}$. The ethanol extract of grape seeds showed more strong lipid oxidation inhibition than that of skin extracts. Similar results were observed in cytotoxic effects. The ethanol extract of grape seeds at 30$\^{C}$ exhibited more strong cytotoxicity than that of skin extracts on MCF-7, Hep3B, and A549 cell lines. Among organic solvent fractions extracted from the ethanol extracts of gape seeds and skins, the hexane fraction showed the strongest cytotoxic inhibition of 75.15% and 62.50% on MCF-7 and Hep3B cell in the presence of 1.0 $\mu\textrm{g}$/㎖ respectively. On the other hand, the water fraction showed the strongest cytotoxic inhibition of 65.41% on A549 cell in the presence of 1.0 $\mu\textrm{g}$/㎖. Overall, the ethanol extracts and their fractions of Black Olympia grape seeds showed strong lipid oxidation inhibition and cytotoxicity than those of grape skins.
Keywords
Black Olympia grape seed and skin; lipid peroxidation inhibition; cytotoxicity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Blavo, L. (1998) Polyphenols: Chemistry, dietary sources, metabolism and nutritional significance. Nutr. Rev. 56, 317-333   DOI   ScienceOn
2 Palma, M., and Taylor, L.T. (1999) Extraction of polyphenolic compounds from grape seeds with near critical carbon dioxide. J. Chromatogr. A. 849, 117-124   DOI   PUBMED   ScienceOn
3 Bagchi, D., Garg, A., Krohn, R.L., Bagchi, M., Bagchi, D.J., Balmoori, J., and Stohs, S.J. (1998) Protective effects of grape seed proanthocyanidins and selected antioxidants against TPA-induced hepatic and brain lipid peroxidation and DNA fragmentation, and peritoneal macrophage activation in mice. Gen. Pharmacol. 30, 771-776   DOI   ScienceOn
4 Zhao, J., Wang, J., Chen, Y., and Agarwal, R. (1999) Anti-tumor-promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3'-gallate as the most effective antioxidant constituent. Carcinogenesis 20, 1737-1745   DOI   ScienceOn
5 Cho, S.Y., Han, Y.B. and Shin, K.H. (2001) Screening for antioxidant activity of edible plants. J. Korean Soc. Food Sci. Nutr. 30, 133-137
6 Kim S.K., Kim Y.G., Lee M.K., Han J.S., Lee J.H., and Lee H.Y. (2000) Comparison of biological activity according to extracting solvents of four Acanthopanax root Bark. J. Medicinal Crop Sci. 8, 21-28
7 Shirataki, Y., Kawase, M., Saito, S., Kurihara, T., Tanaka, W., Satoh, K., Sakagami, H., and Motohashi, N. (2000) Selective cytotoxic activity of grape peel and seed extracts against oral tumor cell lines. Anticancer Res. 20, 423-426
8 Singletary, K.W., and Meline, B. (2001) Effect of grape seed proanthocyanidins on colon aberrant crypts and breast tumors in a rat dual-organ tumor model. Nutr. Cancer. 39, 252-258   DOI   ScienceOn
9 Manzocco, L., Anese, M., and Nicoli, M.C. (1998) Antioxidant properties of tea extracts as affected by processing. Lebensm.-Wiss. U. Technol. 31, 694-698   DOI   ScienceOn
10 Piver, B., Berthou, F., Dreano, Y., and Lucas, D. (2001) Inhibition of CYP3A, CYP1A and CYP2E1 activities by resveratrol and other non volatile red wine components. Toxicology Letters 125, 83-91   DOI   PUBMED   ScienceOn
11 Carnesecchi, S., Schneider, Y., Lazarus, A.S., Coehlo, D., Gosse, F., and Raul, F. (2002) Flavanols and procyanidins of cocoa and chocolate inhibit growth and polyamine biosynthesis of human colonic cancer cells. Cancer Letters 175, 147-155   DOI   ScienceOn
12 Ye, X., Krohn, R.L., Liu, W., Joshi, S.S., Kuszynski, C.A., McGinn, T.R., Bagchi, M., Preuss, H.G., Stohs, S.J., and Bagchi, D. (1999) The cytotoxic effects of a novel IH636 grape seed proanthocyanidin extract on cultured human cancer cells. Mol. Cell Biochem. 196, 99-108   DOI   PUBMED   ScienceOn
13 Bagchi, D., Garg, A., Krohn, R.L., Bagchi, M., Tran, M.X., ad n Stohs, S.J. (1997) Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro. Res. Commun. Mol. Pathol. Pharmacol. 95, 179-189
14 Lee, J.H. and Lee, S.R. (1994) Some physiological activity of phenolic substances in plant foods. Korean J. Food Sci. Technol. 26, 317-323