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http://dx.doi.org/10.3746/jkfn.2017.46.9.1091

Modification and Validation of Analytical Method for Oxypaeoniflorin and Paeoniflorin in Moutan Cortex Radicis Extract  

Choi, Seung-Hyun (Department of Food Science and Biotechnology, Kangwon National University)
Yoo, Chang-Kil (Graduate School of Integrative Medicine, CHA University)
Hwang, Ji-Hyun (Department of Food Science and Biotechnology, CHA University)
Lee, Gi-Bbeum (Department of Food Science and Biotechnology, CHA University)
Lee, Young-Jin (Graduate School of Integrative Medicine, CHA University)
Lee, Boo-Yong (Department of Food Science and Biotechnology, CHA University)
Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.46, no.9, 2017 , pp. 1091-1096 More about this Journal
Abstract
The aim of this study was the validation of a modified analytical method for determination of oxypaeoniflorin and paeoniflorin in Moutan Cortex Radicis extract. For validation of the analytical method, we modified established analytical methods and validated improvement. For validation, the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification of oxypaeoniflorin and paeoniflorin were measured by high performance liquid chromatography. The results show that the correlation coefficients of the calibration curve for oxypaeoniflorin and paeoniflorin were 1.0000 and 0.9998, respectively. The LOD for oxypaeoniflorin and paeoniflorin were $0.23{\mu}g/mL$ and $0.25{\mu}g/mL$, respectively. The inter-day and intra-day precision values of oxypaeoniflorin and paeoniflorin were 0.70~3.19% and 1.74~2.43%, and 0.32~0.92% and 0.62~2.28%, respectively. The inter-day and intra-day accuracies of oxypaeoniflorin and paeoniflorin were 98.33~102.11% and 97.72~118.12%, and 98.44~101.56% and 97.10~112.00%, respectively. Therefore, the analytical method was validated for the detection of oxypaeoniflorin and paeoniflorin in Moutan Cortex Radicis.
Keywords
Moutan Cortex Radicis; oxypaeoniflorin; paeoniflorin; HPLC-PDA; method validation;
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Times Cited By KSCI : 3  (Citation Analysis)
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