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http://dx.doi.org/10.3746/jkfn.2017.46.5.552

Effects of Acanthopanax senticosus Water Extract on Glucose-Regulating Mechanisms in HepG2 Cells  

Kim, Dae-Jung (Well-being Bioproducts RIC, Kangwon National University)
Kang, Yun Hwan (National Development Institute of Korean Medicine)
Kim, Kyoung Kon (Department of Bio-Health Technology, Kangwon National University)
Kim, Tae Woo (Well-being Bioproducts RIC, Kangwon National University)
Park, Jae Bong (Department of Biochemistry, Hallym University College of Medicine)
Choe, Myeon (Well-being Bioproducts RIC, Kangwon National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.46, no.5, 2017 , pp. 552-561 More about this Journal
Abstract
This study aimed to investigate glucose uptake mechanisms and metabolic mechanisms for absorbed glucose in HepG2 cells treated with Acanthopanax senticosus water extract (ASW). A colorimetric assay kit was used to measure polyphenol content, glucokinase (GK) activity, glucose uptake, glucose consumption in cell culture medium, and glycogen content. RT-PCR and western blotting were performed to examine changes in the expression levels of glucose transporter 2 (GLUT2), hepatocyte nuclear factor $1{\alpha}$ ($HNF-1{\alpha}$), phosphatidylinositol 3-kinase (PI3k), protein kinase B (Akt), phospho-AMP-activated protein kinase (AMPK), phosphoenolpyruvate carboxykinase, GK, and glycogen synthase kinase $3{\beta}$ ($GSK3{\beta}$). Increased glucose uptake upon ASW treatment was confirmed to result from increased expression of $HNF-1{\alpha}$, which is one of the transcription factors acting on the GLUT2 promoter. From the measurements of GK activity, we observed that ASW had an effect on glucose phosphorylation, and we also confirmed that increased AMPK phosphorylation promoted glycolysis and suppressed gluconeogenesis. We confirmed that the increase in glycogen upon ASW treatment was induced by activation of Akt by PI3k, followed by phosphorylation of $GSK3{\beta}$. This study demonstrates that ASW activates glucose metabolic mechanisms in liver cells and is therefore a potential candidate to alleviate diabetes.
Keywords
Acanthopanax senticosus; anti-diabetic; glucose transporter; glucokinase; glycogen;
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