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http://dx.doi.org/10.3746/jkfn.2017.46.5.537

Anti-Inflammatory Effect of Ethanolic Extract from Polyopes affinis through Suppression of NF-κB and MAPK Activation in LPS-Stimulated RAW 264.7 Cells  

Kim, Min-Ji (Department of Food Science and Tehcnology/Institute of Food Sciences, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Department of Food Science and Tehcnology/Institute of Food Sciences, Pukyong National University)
Park, Sun-Hee (Department of Food Science and Tehcnology/Institute of Food Sciences, Pukyong National University)
Park, So-Young (Department of Food Science and Tehcnology/Institute of Food Sciences, Pukyong National University)
Choi, Hyeun-Deok (Institute of Fisheries Sciences, Pukyong National University)
Choi, Jung-Su (Subdivision of Culinary Arts, Kyungnam College of Information and Technology)
Jang, Mi-Ran (Health Functional Food Policy Division, Ministry of Food and Drug Safety)
Im, Moo-Hyeog (Department of Food Science and Biotechnology, Daegu University)
Ahn, Dong-Hyun (Department of Food Science and Tehcnology/Institute of Food Sciences, Pukyong National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.46, no.5, 2017 , pp. 537-544 More about this Journal
Abstract
In this study, the anti-inflammatory effect of Polyopes affinis ethanol extract (PAEE) was investigated using LPS-stimulated RAW 264.7 cells and a croton oil-induced ICR mice model. Treatment with PAEE significantly reduced production of nitric oxide (NO) and pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor $(TNF)-{\alpha}$, and $IL-1{\beta}$] in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. PAEE treatment also reduced expression of inducible NO synthase, cyclooxygenase-2, nuclear $factor-{\kappa}B$, and mitogen-activated protein kinases in LPS-stimulated RAW 264.7 cells. In the croton oil-induced ear edema test, application of PAEE (10~250 mg/kg body weight) reduced ear edema in a dose-dependent manner, and PAEE treatment at 50 mg/kg body weight showed similar inhibitory effects compared with prednisolone (10 mg/kg body weight). Histological analysis revealed reduced dermal thickness and lower number of infiltrated mast cells. These results suggest that PAEE might be used as a promising anti-inflammatory agent for inhibition of LPS-induced inflammation and ear edema formation.
Keywords
Polyopes affinis; anti-inflammatory effect; $NF-{\kappa}B$; MAPKs; ear edema;
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