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http://dx.doi.org/10.3746/jkfn.2017.46.4.490

Safety Evaluation of Ethanol Extract from Unripe Fruit of Bitter Melon (Momordica Charantia L.) in Sprague-Dawley Rats  

Ryu, Hyeon Yeol (General Toxicity Evaluation Center, Bio Convergence Technology Laboratory, Korea Conformity Laboratories (KCL))
Lee, Somin (General Toxicity Evaluation Center, Bio Convergence Technology Laboratory, Korea Conformity Laboratories (KCL))
Ahn, Kyu Sup (General Toxicity Evaluation Center, Bio Convergence Technology Laboratory, Korea Conformity Laboratories (KCL))
Yong, Yeon (General Toxicity Evaluation Center, Bio Convergence Technology Laboratory, Korea Conformity Laboratories (KCL))
Kim, Hye Jin (General Toxicity Evaluation Center, Bio Convergence Technology Laboratory, Korea Conformity Laboratories (KCL))
Kim, Seong-Eun (Nutraceutical Food R&D Center, KolmarBNH Co., Ltd.)
Lee, Hak Sung (Nutraceutical Food R&D Center, KolmarBNH Co., Ltd.)
Hong, Su-Young (Nutraceutical Food R&D Center, KolmarBNH Co., Ltd.)
Kim, Hyun-Kyu (Nutraceutical Food R&D Center, KolmarBNH Co., Ltd.)
Hwang, In Guk (Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
Song, Kyung Seuk (General Toxicity Evaluation Center, Bio Convergence Technology Laboratory, Korea Conformity Laboratories (KCL))
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.46, no.4, 2017 , pp. 490-500 More about this Journal
Abstract
This study was performed to evaluate repeated dose oral toxicity upon administration of the test substance 1,2-benzisothiazolin-3-one for 90 days and to determine NOAEL (no observed adverse effect level) and target organs in Sprague-Dawley rats. Single, 2-week repeated, and 13-week repeated oral dose toxicity studies were conducted in Sprague-Dawley rats. The dose levels of groups were 1,250, 2,500, and 5,000 mg/kg/d. All dose groups were compared with the vehicle control group. The animals were observed for clinical signs and weekly body weight. Urinalysis, hematology, and serum biochemistry analyses were conducted. Subsequently, animals were sacrificed and subjected to histopathological examination. For the result, NOAEL of ethanol extract from unripe fruit of bitter melon had an optimal dose of 5,000 mg/kg/d and acceptable daily intake up to 3,000 mg/man. There was no target organ detected. Therefore, bitter melon, which contains a variety of bioactive substances, could be widely used as a health functional food ingredient.
Keywords
Momordica charantia; bitter melon; safety evaluation; NOAEL;
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Times Cited By KSCI : 3  (Citation Analysis)
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