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http://dx.doi.org/10.3746/jkfn.2017.46.11.1286

Protective Effect of 3,5-Dicaffeoylquinic Acid Isolated from Ligularia fischeri against Oxidative Damage in HepG2 Cells  

Park, Sun-Young (Animal Products and Food Science Program, College of Animal Life Sciences, Kangwon National University)
Kim, Gur-Yoo (Animal Products and Food Science Program, College of Animal Life Sciences, Kangwon National University)
Jhoo, Jin-Woo (Animal Products and Food Science Program, College of Animal Life Sciences, Kangwon National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.46, no.11, 2017 , pp. 1286-1292 More about this Journal
Abstract
This study was conducted to investigate the hepatoprotective effects of 3,5-dicaffeoylquinic acid (3,5-DCQA) isolated from Ligularia fischeri against hydrogen peroxide-induced oxidative stress in HepG2 cells. Antioxidative effects of 3,5-DCQA were determined by measuring antioxidant enzyme [superoxide dismutase (SOD), catalase (CAT) glutathione peroxidase (GPx)] expression levels against hydrogen peroxide-induced oxidative stress using real-time PCR analysis. 3,5-DCQA treatment significantly increased gene expression levels of SOD, CAT, and GPx in a dose-dependent manner ($10{\sim}30{\mu}g/mL$) in HepG2 cells. Hepatoprotective effects were analyzed by measuring glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT) activities using a biochemistry analyzer in hydrogen peroxide-treated HepG2 cells. 3,5-DCQA treatment significantly reduced GOT, LDH, and GGT activities in a dose-dependent manner ($10{\sim}30{\mu}g/mL$) against increased liver function index enzyme activities induced by hydrogen peroxide oxidative stress in HepG2 cells. The results reveal that 3,5-DCQA compound isolated from Ligularia fischeri can be useful for the development of an effective hepatoprotective agent.
Keywords
3,5-dicaffeoylquinic acid; Ligularia fischeri; HepG2 cell; antioxidant enzymes; real-time PCR;
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