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http://dx.doi.org/10.3746/jkfn.2016.45.8.1162

Optimization of Extraction of Marker Compounds from Red Ginsengs by Accelerated Solvent Extraction Using Response Surface Methodology  

Kim, Eun Ok (Functional Food Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Xu, Jiu Liang (Functional Food Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Um, Byung Hun (Functional Food Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.45, no.8, 2016 , pp. 1162-1169 More about this Journal
Abstract
A new method based on accelerated solvent extraction (ASE) combined with response surface methodology (RSM) has been developed for optimization of the extraction of ginsenoside [Rb1, Rg1, and Rg3(20S)], total phenolics, and benzopyrene in red ginseng. The RSM method, based on a five level and two variable central composite design, was employed to obtain the optimal combination of extraction conditions. In brief, ginsenosides Rb1, Rg1, and Rg3(20S) and total phenolics with undetectable benzopyrene were optimally extracted with 50% ethanol as an extraction solvent, extraction temperature of $158^{\circ}C$, extraction time of 20 min, extraction pressure of 2,500 psi, flush volume of 60%, and one extraction cycle. The contents of ginsenosides and total phenolics in red ginseng extracted by ASE under optimum conditions were significantly higher than those extracted by sonication and reflux extraction.
Keywords
accelerated solvent extraction; response surface methodology; red ginseng; marker compounds; optimization;
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1 Woo KS, Jang KI, Kim KY, Lee HB, Jeong HS. 2006. Antioxidative activity of heat treated licorice (Glycyrrhiza uralensis Fisch) extracts. Korean J Food Sci Technol 38: 355-360.
2 Kwon OC, Woo KS, Kim TM, Kim DJ, Hong JT, Jeong HS. 2006. Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J Food Sci Technol 38: 331-336.
3 Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS. 2006. Change of physicochemical characteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J Food Sci Technol 38: 342-347.
4 Choi Y, Lee SM, Chun J, Lee HB, Lee J. 2006. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem 99: 381-387.   DOI
5 Dewanto V, Wu X, Liu RH. 2002. Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50: 4959-4964.   DOI
6 Jeong SM, Kim SY, Kim DR, Jo SC, Nam KC, Ahn DU, Lee SC. 2004. Effect of heat treatment on the antioxidant activity of extracts from citrus peels. J Agric Food Chem 52: 3389-3393.   DOI
7 Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee J, Jeong HS. 2006. Change of Korean ginseng components with high temperature and pressure treatment. Korean J Food Sci Technol 38: 521-525.
8 Jung KH, Hong HD, Cho CW, Lee MY, Choi UK, Kim YC. 2012. Phenolic acid composition and antioxidative activity of red ginseng prepared by high temperature and high pressure process. Korean J Food & Nutr 25: 827-832.   DOI
9 Park CK, Jeon BS, Yang JW. 2003. The chemical components of Korean ginseng. Food Industry and Nutrition 8(2): 10-23.
10 Kim EO, Cha KH, Lee EH, Kim SM, Choi SW, Pan CH, Um BH. 2014. Bioavailability of ginsenosides from white and red ginsengs in the simulated digestion model. J Agric Food Chem 62: 10055-10063.   DOI
11 Kong H, Wang M, Venema K, Maathuis A, van der Heijden R, van der Greef J, Xu G, Hankemeier T. 2009. Bioconversion of red ginseng saponins in the gastro-intestinal tract in vitro model studied by high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry. J Chromatogr A 1216: 2195-2203.   DOI
12 Nho JW. 2010. Effect of temperature and solvent on ginseng (Panax ginseng C.A. Meyer) saponin profiling during high temperature and pressure processing. MS Thesis. Chungbuk National University, Cheongju, Korea.
13 Simko P. 2002. Determination of polycyclic aromatic hydrocarbons in smoked meat products and flavouring food additives. J Chromatogr B Analyt Technol Biomed Life Sci 770: 3-18.   DOI
14 Yoon SR, Lee MH, Park JH, Lee IS, Kwon JH, Lee GD. 2005. Changes in physicochemical compounds with heating treatment of ginseng. J Korean Soc Food Sci Nutr 34: 1572-1578.   DOI
15 Dewanto V, Wu X, Adom KK, Liu RH. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50: 3010-3014.   DOI
16 Lee HS, Lee HJ, Yu HJ, Ju DW, Kim Y, Kim CT, Kim CJ, Cho YJ, Kim N, Choi SY, Suh HJ. 2011. A comparison between high hydrostatic pressure extraction and heat extraction of ginsenosides from ginseng (Panax ginseng CA Meyer). J Sci Food Agric 91: 1466-1473.   DOI
17 Box GEP, Wilson KB. 1951. On the experimental attainment of optimum conditions. J Roy Statist Soc 13: 1-45.
18 Park KJ, Lim JH, Kim BK, Jeong JW, Kim JC, Lee MH, Cho YS, Jung H. 2009. Optimization of extraction conditions to obtain functional components from buckwheat (Fagopyrum esculentum M.) sprouts, using response surface methodology. Korean J Food Preserv 16: 734-741.
19 Yoon CH, Bok HS, Choi DK, Row KH. 2012. Optimization condition of astaxanthin extract from shrimp waste using response surface methodology. Korean Chem Eng Res 50: 545-550.   DOI
20 Kim DY, Teng H, Choi YH. 2012. Optimization of ultrasonic-assisted extraction process for Inonotus obliquus using response surface methodology. Curr Res Agric Life Sci 30: 68-75.
21 Csiktusnadi Kiss GA, Forgacs E, Cserhati T, Mota T, Morais H, Ramos A. 2000. Optimisation of the microwave-assisted extraction of pigments from paprika (Capsicum annuum L.) powders. J Chromatogr A 889: 41-49.   DOI
22 Teng H, Lee WY, Choi YH. 2013. Optimization of microwave-assisted extraction for anthocyanins, polyphenols, and antioxidants from raspberry (Rubus coreanus Miq.) using response surface methodology. J Sep Sci 36: 3107-3114.
23 Kown JH, Belanger JM, Pare JR. 2003. Optimization of microwave- assisted extraction (MAP) for ginseng components by response surface methodology. J Agric Food Chem 51: 1807-1810.   DOI
24 Gao F, Hu Y, Ye X, Li J, Chen Z, Fan G. 2013. Optimal extraction and fingerprint analysis of Cnidii fructus by accelerated solvent extraction and high performance liquid chromatographic analysis with photodiode array and mass spectrometry detections. Food Chem 141: 1962-1971.   DOI
25 Zhao LC, He Y, Deng X, Yang GL, Li W, Liang J, Tang QL. 2012. Response surface modeling and optimization of accelerated solvent extraction of four lignans from Fructus Schisandrae. Molecules 17: 3618-3629.   DOI
26 Cha KH, Lee HJ, Koo SY, Song DG, Lee DU, Pan CH. 2010. Optimization of pressurized liquid extraction of carotenoids and chlorophylls from Chlorella vulgaris. J Agric Food Chem 58: 793-797.   DOI
27 Kim JS, Kim KW, Choi KJ, Kwak YK, Im KS, Lee KH, Chung HY. 1996. Screening of antioxidative components from red ginseng saponin. Korean J Ginseng Sci 20: 173-178.
28 Nam KY. 2005. The comparative understanding between red ginseng and white ginsengs processed ginsengs (Panax ginseng C.A. Meyer). J Ginseng Res 29: 1-18.   DOI
29 Shoji J. 1999. Studies on the constituents of ginseng. Nat Med 53: 55-59.
30 Choi JE, Nam KY, Li X, Kim BY, Cho HS, Hwang KB. 2010. Changes of chemical compositions and ginsenoside contents of different root parts of ginsengs with processing method. Korean J Med Crop Sci 18: 118-125.
31 Kim MJ, Jung NP. 1987. The effect of ginseng saponin on the mouse immune system. Korean J Ginseng Sci 11: 130-135.
32 Ha TY, Lee JH, Han JH. 1987. Inhibitory effects of Panax ginseng on tumorigenesis in mice. J Cheonbuk Medical Sci 11: 1-11.
33 Park JH. 2004. Sun ginseng-A new processed ginseng with fortified activity. Food Industry and Nutrition 9(1): 23-27.
34 Park IH, Kim NY, Han SB, Kim JM, Kwon SW, Kim HJ, Park MK, Park JH. 2002. Three new dammarane glycosides from heat processed ginseng. Arch Phrm Res 25: 428-432.   DOI
35 Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299: 152-178.   DOI
36 Hu S, Jin S, Choi D. 2008. Analysis of benzo(a)pyrene in red ginseng beverage. J Fd Hyg Safety 23: 26-30.