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http://dx.doi.org/10.3746/jkfn.2016.45.8.1099

Immunomodulatory Activity of Crude Polysaccharide Separated from Cudrania tricuspidata Leaf  

Byun, Eui-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Jang, Beom-Su (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Sung, Nak-Yun (Department of Food Science and Technology, Kongju National University)
Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.45, no.8, 2016 , pp. 1099-1106 More about this Journal
Abstract
The objective of this study was to evaluate the immunomodulatory activity of crude polysaccharide separated from Cudrania tricuspidata leaf. C. tricuspidata polysaccharide (CTP) was extracted by ethanol precipitation. Immunomodulation activity was tested in macrophage cells (RAW 264.7 and bone-marrow derived macrophage) and splenocytes. CTP treatment significantly increased cell proliferation up to $250{\mu}g/mL$ in both RAW 264.7 and bone-marrow derived macrophages. In this concentration range (below $250{\mu}g/mL$), nitric oxide and cytokine [tumor necrosis factor $(TNF)-{\alpha}$ and interleukin (IL)-6] production also significantly increased. Similarly, splenocyte proliferation dosedependently increased except for the $1,000{\mu}g/mL$ treated group. Regarding cytokine production activity in splenocytes, CTP treatment significantly increased production of Th 1 type cytokines [interferon $(IFN)-{\gamma}$] production but not Th 2 type cytokines (IL-4). Therefore, the results indicate that CTP may have a potential effect on immunomodulatory activity in various immune cells, and this is useful for development of immune enhancing adjuvant materials as a natural ingredient.
Keywords
Cudrania tricuspidata; polysaccharide; immunomodulatory activity; macrophage cells; splenocyte;
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