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http://dx.doi.org/10.3746/jkfn.2016.45.7.973

Polyphenol Contents and Antioxidant Activities of Lentil Extracts from Different Cultivars  

Lee, So-Hee (Department of Food Science and Technology, Keimyung University)
Lee, Syng-Ook (Department of Food Science and Technology, Keimyung University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.45, no.7, 2016 , pp. 973-979 More about this Journal
Abstract
Lentils (Lens culinaris) have been gaining increasing attention recently as a top five superfood, as they are high in protein and other essential nutrients, including folate, iron, potassium, and various antioxidants. In the present study, phenolic extracts from four different lentil cultivars (green, red, French, and beluga) were evaluated for their total phenolic contents and in vitro antioxidant activities. Total polyphenol and flavonoid contents of four different lentil extracts were 27.30~30.30 mg tannic acid equivalents (TAE)/g and 13.14~16.29 mg quercetin equivalents (QUE)/g, respectively. Beluga and red lentil extracts showed higher polyphenol contents than others (P<0.05), whereas there was no significant difference in flavonoid contents among the four lentil cultivars. $RC_{50}$ values of the lentil extracts for DPPH radical, ABTS radical, and $H_2O_2$ were $57.42{\sim}64.49{\mu}g/mL$, $66.11{\sim}75.69{\mu}g/mL$, and $59.72{\sim}72.86{\mu}g/mL$, respectively. Among the four lentil extracts, beluga lentil extract showed the most potent scavenging effect in all three reactive oxygen species (ROS) scavenging assays, and thus beluga extract was further tested for its inhibitory effect on early peroxidation of linoleic acid. The results showed that beluga lentil extract significantly inhibited linoleic acid peroxidation in a dose-dependent manner (concentration required for 50% reduction=$222.76{\m}g/mL$). In addition, beluga lentil extract showed a significant protective effect against alcohol-induced cytotoxicity in AML-12 cells (normal mouse hepatocyte cell line). Taken together, these results suggest that lentil extracts represent potential sources of natural antioxidants, and further studies will be necessary to determine their protective effects against oxidative stress in vivo.
Keywords
lentil; polyphenols; antioxidant; reactive oxygen species;
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1 Goldberg I. 1994. Functional foods. Chapman & Hall Press, New York, NY, USA. p 3-550.
2 Nicoletti M. 2012. Nutraceuticals and botanicals: overview and perspectives. Int J Food Sci Nutr 63(S1): 2-6.   DOI
3 Nam SY, Hong JT, Yun YW, Ahn B, Lee BJ. 2004. Occurrence and measurement of reactive oxygen species in biological systems. J Vet Med Biotechnol 5: 5-14.
4 Choi DS, Go HY. 1995. Chemistry of functional food. Ji-Gu Publishing Co., Paju, Korea. p 78-79.
5 Kim TS, Kang SJ, Park WC. 1999. Changes in antioxidant and antioxidant enzymes activities of soybean leaves subjected to water stress. J Korean Soc Agric Chem Biotechnol 42: 246-251.
6 Thavarajah D, Thavarajah P, Sarker A, Vandenberg A. 2009. Lentils (Lens culinaris Medikus Subspecies culinaris): a whole food for increased iron and zinc intake. J Agric Food Chem 57: 5413-5419.   DOI
7 Hefnawy TH. 2011. Effect of processing methods on nutritional composition and anti-nutritional factors in lentils (Lens culinaris). Ann Agric Sci 56: 57-61.
8 Galleano M, Pechanova O, Fraga CG. 2010. Hypertension, nitric oxide, oxidants, and dietary plant polyphenols. Curr Pharm Biotechnol 11: 837-848.   DOI
9 Xu BJ, Yuan SH, Chang SKC. 2007. Comparative analyses of phenolic composition, antioxidant capacity, and color of cool season legumes and other selected food legumes. J Food Chem 72: S167-S177.   DOI
10 Aguilera Y, Duenas M, Estrella I, Hernandez T, Benitez V, Esteban RM, Martin-Cabrejas MA. 2010. Evaluation of phenolic profile and antioxidant properties of Pardina lentil as affected by industrial dehydration. J Agric Food Chem 58: 10101-10108.   DOI
11 Singleton VL. 1981. Naturally occurring food toxicants: phenolic substances of plant origin common in foods. Adv Food Res 27: 149-242.   DOI
12 Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71: 109-114.   DOI
13 Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.   DOI
14 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237.   DOI
15 Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung Island. Korean J Food Sci Technol 37: 233-240.
16 Nakatani N, Kikuzaki H. 1987. A new antioxidative glucoside isolated from oregano (Origanum vulgare L.). Agric Biol Chem 51: 2727-2732.
17 Velioglu YS, Mazza G, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46: 4113-4117.   DOI
18 Zhang B, Deng Z, Ramdath DD, Tang Y, Chen PX, Liu R, Liu Q, Tsao R. 2015. Phenolic profiles of 20 Canadian lentil cultivars and their contribution to antioxidant activity and inhibitory effects on ${\alpha}$-glucosidase and pancreatic lipase. Food Chem 172: 862-872.   DOI
19 Khan MA, Ammar MH, Migdadi HM, El-Harty EH, Osman MA, Farooq M, Alghamdi SS. 2015. Comparative nutritional profiles of various faba bean and chickpea genotypes. Int J Agric Biol 17: 449-457.   DOI
20 Fratianni F, Cardinale F, Cozzolino A, Granese T, Albanese D, Matteo MD, Zaccardelli M, Coppola R, Nazzaro F. 2014. Polyphenol composition and antioxidant activity of different grass pea (Lathyrus sativus), lentils (Lens culinaris), and chickpea (Cicer arietinum) ecotypes of the Campania region (Southern Italy). J Funct Foods 7: 551-557.   DOI
21 Oomah BD, Mazza G. 1996. Flavonoids and antioxidative activities in buckwheat. J Agric Food Chem 44: 1746-1750.   DOI
22 Amarowicz R, Estrella I, Hernandez T, Duenas M, Troszynska A, Agnieszka K, Pegg RB. 2009. Antioxidant activity of a red lentil extract and its fractions. Int J Mol Sci 10: 5513-5527.   DOI
23 Kim JP, Yang YS, Kim JH, Lee HH, Kim ES, Moon YW, Kim JY, Chung JK. 2012. Chemical properties and DPPH radical scavenging ability of sword bean (Canavalia gladiata) extract. Korean J Food Sci Technol 44: 441-446.   DOI
24 Ademiluyi AO, Oboh G. 2012. Attenuation of oxidative stress and hepatic damage by some fermented tropical legume condiment diets in streptozotocin-induced diabetes in rats. Asian Pac J Trop Med 5: 692-697.   DOI
25 Finkel T, Holbrook NJ. 2000. Oxidants, oxidative stress and the biology of ageing. Nature 408: 239-247.   DOI
26 Das SK, Vasudevan DM. 2007. Alcohol-induced oxidative stress. Life Sci 81: 177-187.   DOI
27 Jung GT, Ju IO, Choi JS, Hong JS. 2000. The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis RUPRECHT (Omija) seed. Korean J Food Sci Technol 32: 928-935.