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http://dx.doi.org/10.3746/jkfn.2016.45.6.918

Antioxidant and Anti-Proliferative Activities of Oats under Different Solvent Extraction Conditions  

Ham, Hyeonmi (Department of Central Area, National Institute of Crop Science, Rural Development Administration)
Woo, Koan Sik (Department of Central Area, National Institute of Crop Science, Rural Development Administration)
Park, Ji-Young (Department of Central Area, National Institute of Crop Science, Rural Development Administration)
Lee, Byongwon (Department of Central Area, National Institute of Crop Science, Rural Development Administration)
Choi, Yong-Hwan (Department of Central Area, National Institute of Crop Science, Rural Development Administration)
Lee, Choonwoo (Department of Central Area, National Institute of Crop Science, Rural Development Administration)
Kim, Wook Han (Department of Central Area, National Institute of Crop Science, Rural Development Administration)
Lee, Junsoo (Department of Food Science and Biotechnology, Chungbuk National University)
Lee, Yu-Young (Department of Central Area, National Institute of Crop Science, Rural Development Administration)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.45, no.6, 2016 , pp. 918-922 More about this Journal
Abstract
The objective of this study was to determine the antioxidant and anti-proliferative activities of methanol, ethanol, acetone, and ethyl acetate extracts from oats (Avena sativa L.). Total polyphenol contents of extracts were analyzed by Folin-Ciocalteu assay. The antioxidant activities of extracts were determined by 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities and reducing power. The anti-proliferative activities of colon (HCT116), lung (NCI-H460), and breast (MCF7) cancer cells were investigated. Among solvents, methanol extract showed the highest amount of total polyphenols, which was 8.2 mg gallic acid equivalents/g residue. High levels of ABTS radical [12.1 mg Trolox equivalent antioxidant capacity (TEAC)/g residue] and DPPH radical (4.4 mg TEAC/g residue) scavenging activity and reducing power ($A_{700}=0.39$) were found in methanol extracts. Moreover, methanol extracts indicated higher anti-proliferative activities against HCT116 (69.5%), NCI-H460 (75.2%), and MCF7 (84.8%) cells compared with other extracts. The results show that methanol was the best solvent for extraction of antioxidant and anti-proliferative compounds from oats. Moreover, notable antioxidant and anti-proliferative activities of oats could have significant health benefits.
Keywords
oat; polyphenolics; antioxidant; antiproliferation;
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