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http://dx.doi.org/10.3746/jkfn.2016.45.11.1589

Induction of Apoptosis and Inhibition of Growth in Human Gastric Cancer by Piperine  

Shin, Seong-Ah (Department of Companion and Laboratory Animal Science, Kongju National University)
Lee, Hae-Nim (Department of Companion and Laboratory Animal Science, Kongju National University)
Choo, Gang-Sik (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, So-Jung (Department of Program in Cell Biology and Genetics, College of Medicine, Chungbuk National University)
Kim, Hyeong-Jin (Department of Companion and Laboratory Animal Science, Kongju National University)
Park, Young-Seok (Department of Companion and Laboratory Animal Science, Kongju National University)
Park, Byung-Kwon (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Byeong-Soo (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Sang-Ki (Department of Companion and Laboratory Animal Science, Kongju National University)
Lee, Hu-Jang (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.45, no.11, 2016 , pp. 1589-1594 More about this Journal
Abstract
Piperine [(E,E)-5-(3,4-methtylenedioxyphenyl)-2,4-pentadienolypiperidide] is a principal of Piperaceae, including Piper nigrum L. and Piper longum Linn., which has been used as a spice and traditional medicine. In this study, we investigated whether or not piperine has anti-cancer effects on AGS human gastric cancer cells. The results demonstrated that piperine not only inhibited proliferation using MTT assay but also induced apoptotic bodies using DAPI assay in a dose-dependent manner in response to piperine. Expression levels of p53, Bax (pro-apoptotic), cleaved caspase-9, and cleaved-PARP increased, whereas expression levels of Bcl-2, XIAP (anti-apoptotic), and Akt decreased in a dose-dependent manner compared with the control by western blotting analysis. To identify the connection between phospo-Akt and Bcl-2 family in response to piperine, LY249002 (Akt inhibitor) was treated with piperine ($150{\mu}M$). The results were shown that expression of phospo-Akt was reduced whereas expression of Bax and cleaved-PARP increased in a dose-dependent manner. These results indicate that piperine induced apoptosis in AGS cells and may serve as a chemopreventive or therapeutic agent for human gastric cancer.
Keywords
cancer cell; apoptosis; anticancer; piperine; Akt signaling;
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