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http://dx.doi.org/10.3746/jkfn.2016.45.11.1544

Calcium Bioavailability and Antiosteoporotic Effects of Cheonggukjang Containing High Poly-Gamma Glutamate Contents  

Lee, Mu-Jin (Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine)
Jung, Ho-Kyung (Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine)
Sim, Mi-Ok (Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine)
Jang, Ji-Hun (Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine)
Kim, Tae-Mook (Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine)
Lee, Hyun-Joo (Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine)
Lee, Ki-Ho (Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine)
An, Byeong-Kwan (Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine)
Cho, Jung-Hee (Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine)
Jang, Min-Cheol (Heanam Natural Farming Association Corporation)
Yong, Ju-Hyun (Heanam Natural Farming Association Corporation)
Kim, Jong-Choon (College of Veterinary Medicine BK21 Plus Project Team, Chonnam National University)
Cho, Hyun-Woo (Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.45, no.11, 2016 , pp. 1544-1551 More about this Journal
Abstract
Various types of fermented soybean foods are consumed in various Asian countries, including China, Indonesia, Japan, Korea, and Vietnam. Cheonggukjang is a Korean whole soybean paste fermented by Bacillus subtilis and regarded as a healthy food. The objective of this study was to investigate the Ca-bioavailability and anti-osteoporotic effects of cheonggukjang (CGJ) containing high poly-gamma glutamate (PGA) contents in an animal model. Blood samples were collected from the jugular vein at 0, 0.5, 1, 2, 3, 4, and 5 h after a single oral dose in male rats. Ca-bioavailability in CGJ containing high PGA contents was approximately 3~5 times higher than that of natural CGJ. Female rats were either sham-operated (sham; n=5) or surgically ovariectomized (OVX; five animals per group) and then administered to OVX control, raloxifene hydrochloride (RLX) 1 mg/kg/d, or CGJ containing high contents of PGA (20 and 200 mg/kg/d) for 12 weeks. Serum osteocalcin concentration was significantly lower in the CGJ 200 mg/kg/d group compared with the OVX control group. Serum progesterone concentration was significantly higher in the CGJ 200 mg/kg/d group compared with the OVX control group. Reduction grade of the trabecular bone decreased in the RLX 1 and CGJ 200 mg/kg/d group compared with that of the OVX control group. In conclusion, CGJ 200 mg/kg/d may have inhibitory effects on osteoporosis in OVX rats, and Ca-bioavailability was improved in CGJ containing high PGA contents.
Keywords
cheonggukjang; calcium bioavailability; ovariectomized; osteocalcin; progesterone;
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Times Cited By KSCI : 10  (Citation Analysis)
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1 Booth SL, Suttie JW. 1998. Dietary intake and adequacy of vitamin K. J Nutr 128: 758-788.   DOI
2 Akedo Y, Hosoi T, Inoue S, Ikegami A, Mizuno Y, Kaneki M, Nakamura T, Ouchi Y, Orimo H. 1992. Vitamin $K_2$ modulates proliferation and function of osteoblastic cells in vitro. Biochem Biophys Res Commun 187: 814-820.   DOI
3 Lee KH, Ryu SH, Lee YS, Kim YM, Moon GS. 2005. Changes of antioxidative activity and related compounds on the Chungkukjang preparation by adding drained boiling water. Korean J Food Cook Sci 21: 163-170.
4 Choi YB, Sohn HS. 1998. Isoflavone content in Korean fermented and unfermented soybean foods. Korean J Food Sci Technol 30: 745-750.
5 Chung WY, Kim SK, Son JY. 2008. Isoflavones contents and physiological activities of soybeans fermented with Aspergillus oryzae or Bacillus natto. J Korean Soc Food Sci Nutr 37: 141-147.   DOI
6 Somekawa Y, Chiguchi M, Ishibashi T, Aso T. 2001. Soy intake related to menopausal symptoms, serum lipids, and bone mineral density in postmenopausal Japanese women. Obstet Gynecol 97: 109-115.
7 Morabito N, Crisafulli A, Vergara C, Gaudio A, Lasco A, Frisina N, D'Anna R, Corrado F, Pizzoleo MA, Cincotta M, Altavilla D, Ientile R, Squadrito F. 2002. Effects of genistein and hormone-replacement therapy on bone loss in early postmenopausal women: a randomized double-blind placebo- controlled study. J Bone Miner Res 17: 1904-1912.   DOI
8 Taku K, Umegaki K, Sato Y, Taki Y, Endoh K, Watanabe S. 2007. Soy isoflavones lower serum total and LDL cholesterol in humans: a meta-analysis of 11 randomized controlled trials. Am J Clin Nutr 85: 1148-1156.   DOI
9 Akesson K, Lau KH, Baylink DJ. 1997. Rationale for active vitamin D analog therapy in senile osteoporosis. Calcif Tissue Int 60: 100-105.   DOI
10 Abadjieva DK, Kang SK. 1996. Effects of Cervi Cornu aquaacupuncture on lipid metabolism and osteoporosis in ovariectomized rats. J Korean Orient Med 17: 168-177.
11 Kim SJ. 2003. Study on the relationship between osteoporosis- cause factor and bone mineral density, biochemical marker. MS Thesis. Pukyong National University, Busan, Korea. p 28-29.
12 Liu G, Peacock M. 1998. Age-related changes in serum undercarboxylated osteocalcin and its relationships with bone density, bone quality, and hip fracture. Calcif Tissue Int 62: 286-289.   DOI
13 McDonald LE, Pineda MH. 1989. Veterinary endocrinology and reproduction. 4th ed. Lea and Febiger Philadelphia, PA, USA. p 303-354.
14 Heersche JN, Bellows CG, Ishida Y. 1998. The decrease in bone mass associated with aging and menopause. J Prosthet Dent 79: 14-16.   DOI
15 Lee O. 2004. Effects of supplementation of Puerariae radix ethanol extract on the antioxidative defense system in rats. Korean J Nutr 37: 872-880.
16 Thurman RG, Bradford BU, Iimuro Y, Knecht KT, Connor HD, Adachi Y, Wall C, Arteel GE, Raleigh JA, Forman DT, Mason RP. 1977. Role of Kupffer cells, endotoxin and free radicals in hepatotoxicity due to prolonged alcohol consumption: studies in female and male rats. J Nutr 127: 903S-906S.
17 Morel DW, Chisolm GM. 1989. Antioxidant treatment of diabetic rats inhibits lipoprotein oxidation and cytotoxicity. J Lipid Res 30: 1827-1834.
18 Kimmel DB. 1996. Animal models for in vivo experimentation in osteoporosis research. Osteoporosis Academic Press, San Diego, CA, USA. p 671-690.
19 Black LJ, Sato M, Rowley ER, Magee DE, Bekele A, Williams DC, Cullinan GJ, Bendele R, Kauffman RF, Bensch WR, Frolik CA, Termine JD, Bryant HU. 1994. Raloxifene (LY139481 HCI) prevents bone loss and reduces serum cholesterol without causing uterine hypertrophy in ovariectomized rats. J Clin Invest 93: 63-69.   DOI
20 Bang BH, Rhee MS, Kim KP, Yi DH. 2012. Influences of culture medium components on the production poly(${\gamma}$-glutamic acid) by Bacillus subtilis GS-2 isolated Chungkookjang. Korean J Food Nutr 25: 677-684.   DOI
21 Lee KH, Sim MO, Song YS, Jung HK, Jang JH, Kim MS, Kim TM, Lee HE, An BK, Jung WS. 2016. Effects of polygamma glutamate contents Cheonggukjang on osteoblast differentiation. J Korean Soc Food Sci Nutr 45: 664-670.   DOI
22 Allen LH. 1982. Calcium bioavailability and absorption: a review. Am J Clin Nutr 35: 783-808.   DOI
23 Pansu D, Bellaton C, Bronner F. 1981. Effect of Ca intake on saturable and nonsaturable components of duodenal Ca transport. Am J Physiol 240: G32-G37.
24 Bronner F. 1992. Current concepts of calcium absorption: an overview. J Nutr 122: 641-643.   DOI
25 Park C, Chol YH, Shin HJ, Poo HY, Song JJ, Kim CJ, Sung MH. 2005. Effect of high-molecular-weight poly-${\gamma}$-glutamic acid from Bacillus subtilis (chungkookjang) on Ca solubility and intestinal absorption. J Microbiol Biotechnol 15: 855-858.
26 Han KO. 2005. Molecular pathophysiology of postmenopausal osteoporosis. J Korean Soc Menopause 11: 175-177.   DOI
27 Li M, Wronski TJ. 1995. Response of femoral neck to estrogen depletion and parathyroid hormone in aged rats. J Bone Miner Res 16: 551-557.
28 Johnell O, Oden A, De Laet C, Garnero P, Delmas PD, Kanis JA. 2002. Biochemical indices of bone turnover and the assessment of fracture probability. Osteoporos Int 13: 523-526.   DOI
29 Park HM. 2010. Current use of drugs for osteoporosis in Korea. Korean J Obstet Gynecol 53: 152-159.   DOI
30 Walsh CA, Birch MA, Fraser WD, Ginty AF, Gallagher JA. 2000. Cytokine expression by cultured osteoblasts from patients with osteoporotic fractures. Int J Exp Path 81: 159-163.
31 Lee JW, Kim H, Jhee O, Won H, Yu Y, Lee M, Kim T, Om A, Kang J. 2005. Effects of alternative medicine extract on bone mineral density, bone strength and biochemical markers of bone metabolism in ovariectomized rats. Korean J Food Nutr 18: 72-80.
32 Kim YM, Kim JH, Cho DS. 2015. Gender difference in osteoporosis prevalence, awareness and treatment: based on the Korea national health and nutrition examination survey 2008-2011. J Korean Acad Nurs 45: 293-305.   DOI
33 Choi HJ. 2013. Treatment for osteoporosis: focusing on bisphosphonate therapy. Korean J Fam Pract 3: 16-24.
34 Chung HY. 2008. Osteoporosis diagnosis and treatment 2007. J Korean Endocr Soc 23: 76-108.   DOI
35 Kanis JA, Melton LJ, Christiansen C, Johnston CC, Khaltaev N. 1994. The diagnosis of osteoporosis. J Bone Min Res 9: 1137-1141.
36 Grodstein F, Stampfer MJ, Colditz GA, Willett WC, Manson JE, Joffe M, Rosner B, Fuchs C, Hankinson SE, Hunter DJ, Hennekens CH, Speizer FE. 1997. Postmenopausal hormone therapy and mortality. N Engl J Med 336: 1769-1775.   DOI
37 Kang MJ, Cho JY, Shim BH, Kim DK, Lee J. 2009. Bioavailability enhancing activities of natural compounds from medicinal plants. J Med Plant Res 13: 1204-1211.
38 Tanimoto H, Mori M, Motoki M, Torii K, Kadowaki M, Noguchi T. 2001. Natto mucilage containing poly-${\gamma}$-glutamic acid increases soluble calcium in the rat small intestine. Biosci Biotechnol Biochem 65: 516-521.   DOI
39 Lee JO, Ha SD, Kim AJ, Yuh CS, Bang IS, Park SH. 2005. Industrial application and physiological functions of Chongkukjang. Food Science and Industry 38(2): 69-78.
40 Kang SA, Jang KH, Cho Y, Hong K, Suh JH, Choue R. 2003. Effects of artificial stomach fluid and digestive enzymes on the aglycone isoflavone contents of soybean and black bean (Rhynchosia molubillis: Yak-Kong). Korean J Nutr 36: 32-39.
41 Lee MS, Kang JI, Kim HS. 2006. Effect of ${\gamma}$-PGA(poly-${\gamma}$- glutamic acid) supplement on calcium absorption and bone metabolism in rats. J Korean Soc Food Sci Nutr 35: 255-261.   DOI
42 Louie DS. 1996. Calcium and phosphorus in health and disease: Intestinal bioavailability and absorption of calcium. CRC Press, Boca Raton, FL, USA. p 45.