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http://dx.doi.org/10.3746/jkfn.2015.44.8.1144

Antioxidant Activities and Antimicrobial Effects of Solvent Extracts from Lentinus edodes  

Han, So-Ra (Department of Pharmaceutical Engineering, SunMoon University)
Kim, Mi-Jin (Department of Pharmaceutical Engineering, SunMoon University)
Oh, Tae-Jin (Department of Pharmaceutical Engineering, SunMoon University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.44, no.8, 2015 , pp. 1144-1149 More about this Journal
Abstract
The aim of this study was to investigate the antioxidant and antimicrobial activities of various solvent (acetone, ethyl acetate, and ethanol) extracts from Lentinus edodes. The antioxidant activities were evaluated by measuring total polyphenol and flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity. Total polyphenol content and ABTS radical scavenging activity were highest in ethanol extract. ABTS radical scavenging activity of ethanol extract showed the highest value (98.5%), which was similar to that of ascorbic acid (95.7%). The ethyl acetate extract from Lentinus edodes showed relatively high total flavonoid content and DPPH radical scavenging activity. Negative correlations were found between total polyphenol contents and DPPH radical scavenging activities in Lentinus edodes extracts. Antimicrobial activities of the extracts were determined against Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae by the disc diffusion method. The acetone and ethanol extracts showed moderate antimicrobial activities against almost all tested microorganisms except E. coli and S. aureus, respectively. The ethyl acetate extract showed a significant growth inhibition effect against E. coli, Ent. cloacae, and B. subtilis.
Keywords
ABTS; antimicrobial effect; antioxidant activity; DPPH; Lentinus edodes;
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Times Cited By KSCI : 11  (Citation Analysis)
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1 Qi Y, Zhao X, Lim YI, Park KY. 2013. Antioxidant and anticancer effects of edible and medicinal mushrooms. J Korean Soc Food Sci Nutr 42: 655-662.   DOI   ScienceOn
2 Kim MJ, Chu WM, Park EJ. 2012. Antioxidant and antigenotoxic effects of shiitake mushrooms affected by different drying methods. J Korean Soc Food Sci Nur 41: 1041-1048.   DOI   ScienceOn
3 Kim YD, Kim KJ, Cho DB. 2003. Antimicrobial activity of Lentinus edodes extract. Korean J Food Preserv 10: 89-93.
4 Folin O, Denis W. 1915. A colorimetric method for determination of phenols (and phenol derivatives) in urine. J Biol Chem 22: 305-308.
5 Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.   DOI
6 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237.   DOI
7 Piddock LJ. 1990. Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. J Appl Bacteriol 68: 307-318.   DOI
8 Di Carlo G, Mascolo N, Izzo AA, Capasso F. 1999. Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci 65: 337-353.   DOI
9 Jankun J, Selman SH, Swiercz R, Skrzypczak-Jankun E. 1997. Why drinking green tea could prevent cancer. Nature 387: 561.   DOI
10 Cheung LM, Cheung PCK, Ooi VEC. 2003. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem 81: 249-255.   DOI
11 Ferreira IC, Barros L, Abreu RM. 2009. Antioxidants in wild mushrooms. Curr Med Chem 16: 1543-1560.   DOI
12 Kim SM, Cho YS, Sung SK. 2001. The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol 33: 626-632.
13 Que F, Mao L, Zhu C, Xie G. 2006. Antioxidant properties of Chinese yellow wine, its concentrate and volatiles. LWT -Food Sci Technol 39: 111-117.   DOI
14 Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28: 232-239.
15 Choi CW, Kim SC, Hwang SS, Choi BK, Ahn HJ, Lee MY, Park SH, Kim SK. 2002. Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Science 163: 1161-1168.   DOI
16 Gallardo C, Jeimenz L, Garcia-Conesa MT. 2006. Hydroxycinnamic acid composition and in vitro antioxidant activity of selected grain fractions. Food Chem 99: 455-463.   DOI
17 Vundac VB, Brantner AH, Plazibat M. 2007. Content of polyphenolic constituents and antioxidant activity of some Stachys taxa. Food Chem 104: 1277-1281.   DOI
18 Hong MH, Jin YJ, Pyo YH. 2012. Antioxidant properties and ubiquinone contents in different parts of several commercial mushrooms. J Korean Soc Food Sci Nutr 41: 1235-1241.   DOI   ScienceOn
19 Huang D, Ou B, Prior RL. 2005. The chemistry behind antioxidant capacity assays. J Agric Food Chem 53: 1841-1856.   DOI
20 Jin SY. 2011. Study on antioxidant activities of extracts from different parts of Korean and Iranian pomegranates. J Korean Soc Food Sci Nutr 40: 1063-1072.   DOI   ScienceOn
21 Yim SB, Kim MO, Koo SJ. 1991. Determination of dietary fiber contents in mushrooms. Korean J Soc Food Sci 7:69-76.
22 Lee JS, Kim T, Lee YH, Jin CM, Kim HG, Kim WJ, Oh DC, Park YI. 2006. Antimicrobial activity of the Coriolus versicolor liquid culture extracts against antibiotic resistant bacteria and purification of active substance. Korean J Mycol 34: 92-97.   DOI
23 Kim HJ, Ahn MS, Kim GH, Kang MH. 2006. Antioxidative and antimicrobial activities of Pleurotus eryngii extracts prepared from different aerial part. Korean J Food Sci Technol 38: 799-804.
24 Park JW, Kim T, Lim DJ, Lee HB, Joo YS, Park YI. 2004. Antibacterial activities of mushroom liquid culture extracts against livestock disease-causing bacteria and antibiotic resistant bacteria. Korean J Mycol 32: 145-147.   DOI
25 Choi OK, Kim Y, Cho GS, Sung CK. 2002. Screening for antimicrobial activity from Korean plants. Korean J Food & Nutr 15: 300-306.
26 Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39: 44-84.   DOI
27 Mizuno T. 1999. The extraction and development of antitumor-active polysaccharides from medicinal mushrooms in Japan. Int J Med Mushrooms 1: 9-29.   DOI
28 Chaovanalikit A, Wrolstad RE. 2004. Total anthocyannins and total phenolics of fresh and processed cherries and their antioxidant properties. J Food Sci 69: 67-72.
29 Park MH, Oh KY, Lee BW. 1998. Anti-cancer activity of Lentinus edodes and Pleurotus astreatus. Korean J Food Sci Technol 30: 702-708.
30 Lee IS, Chae HJ, Moon H. 2008. Inhibitory effect of aqueous extracts from the fruit body of Lentinus edodes on rat intestinal mucosa ${\alpha}$-glucosidase activity and reducing the increase of blood glucose after Streptozotocin-induced diabetic rats. J Exp Biomed Sci 14: 63-68.
31 Enman J, Rova U, Berglund KA. 2007. Quantification of the bioactive compound eritadenine in selected strains of shiitake mushroom (Lentinus edodes). J Agric Food Chem 55:1177-1180.   DOI
32 Hatvani N. 2001. Antibacterial effect of the culture fluid of Lentinus edodes mycelium grown in submerged liquid culture. Int J Antimicrob Agents 17: 71-74.   DOI
33 Kim H, You J, Jo Y, Lee Y, Park I, Park J, Jung MA, Kim YS, Kim S. 2013. Inhibitory effects of Lentinus edodes and rice with Lentinus edodes mycelium on diabetes and obesity. J Korean Soc Food Sci Nutr 42: 175-181.   DOI   ScienceOn
34 Lee GD, Chang HG, Kim HK. 1997. Antioxidative and nitrite-scavenging activities of edible mushroom. Korean J Food Sci Technol 29: 432-436.
35 Kim CH, Jeong JG. 2009. Antioxidant activities and the effect of reducing serum alcohol concentration of Lentinus edodes. Kor J Herbology 24: 159-164.