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http://dx.doi.org/10.3746/jkfn.2015.44.8.1128

Anti-Inflammatory Effect of Ethanol Extract from Grateloupia elliptica Holmes on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells and Mice Ears  

Bae, Nan-Young (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Min-Ji (Institute of Fisheries Sciences, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University)
Ahn, Na-Kyung (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Choi, Yeon-Uk (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Park, Ji-Hye (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Park, Sun-Hee (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.44, no.8, 2015 , pp. 1128-1136 More about this Journal
Abstract
This study investigated the anti-inflammatory effects of ethanol extract from Grateloupia elliptica Holmes (GEHEE) on the lipopolysaccharide-induced inflammatory response. Anti-inflammatory effects were detected by enzyme-linked immunosorbent assay, Western blotting, and immunohistochemistry. There were no cytotoxic effects on proliferation of macrophages treated with GEHEE compared to the control. GEHEE remarkably suppressed NO and pro-inflammatory cytokines (interleukin-6, tumor necrosis $factor-{\alpha}$, and $interleukin-1{\beta}$) production and reduced expression of inducible nitric oxide synthase, cyclooxygenase-2, and nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) proteins in a dose-dependent manner. GEHEE also significantly reduced activation of mitogen-activated protein kinases (MAPKs). The formation of edema in mouse ears was reduced at the highest dose compared to the control. GEHEE also reduced dermal thickness and mast cell numbers based on histological analysis. These results suggest that GEHEE exerts significant anti-inflammatory activity via inhibition of $NF-{\kappa}B$ and MAPKs activation and may be a potential anti-inflammatory therapeutic material.
Keywords
Grateloupia elliptica Holmes; anti-inflammatory activity; $NF-{\kappa}B$; MAPKs; ear edema;
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Times Cited By KSCI : 13  (Citation Analysis)
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