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http://dx.doi.org/10.3746/jkfn.2015.44.5.664

Chemical Properties and Immuno-Stimulating Activities of Crude Polysaccharides from Enzyme Digests of Tea Leaves  

Park, Hye-Ryung (Department of Food Science and Biotechnology, Kyonggi University)
Suh, Hyung Joo (Department of Integrated Biomedical and Life Science, College of Health Science, Korea University)
Yu, Kwang-Won (Department of Food and Nutrition, Korea University of Transportation)
Kim, Tae Young (Bionic Trading Corporation)
Shin, Kwang-Soon (Department of Food Science and Biotechnology, Kyonggi University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.44, no.5, 2015 , pp. 664-672 More about this Journal
Abstract
In order to develop new immuno-stimulating ingredients from mature leaves of green tea, crude polysaccharides were isolated from pectinase digests of tea leaves (green tea enzyme digestion, GTE-0), after which their immuno-stimulating activities and chemical properties were examined. GTE-0 mainly contained neutral sugars (54.9%) such as glucose (14.2%), arabinose (12.2%), rhamnose (11.1%), and galacturonic acid (45.1%), which are characteristic of pectic polysaccharides. The anti-complementary activity of GTE-0 was similar to that of polysaccharide K (used as positive control). Number of morphologically activated macrophages was significantly increased in the GTE-0-treated group. GTE-0 significantly augmented $H_2O_2$ and reactive oxygen species production by murine peritoneal macrophage cells in a dose-dependent manner, whereas production of nitric oxide showed the highest activity at a dose of $100{\mu}g/mL$ among all tested concentrations. Murine peritoneal macrophages stimulated with GTE-0 showed enhanced production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factors-${\alpha}$ in a dose-dependent manner. Further, GTE-0 induced higher phagocytic activity in a dose-dependent manner. In ex vivo assay for cytolytic activity of murine peritoneal macrophages, GTE-0-treated group showed significantly higher activity compared to the untreated group at an effector-to-target cell ratio of 20. The above results lead us to conclude that polysaccharides from leaves of green tea have a potent immuno-stimulating effect on murine peritoneal macrophage cells.
Keywords
green tea; polysaccharide; macrophage; cytokine; complement;
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