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http://dx.doi.org/10.3746/jkfn.2015.44.5.657

Radioprotective Effects of Post-Treatment with Hesperetin against γ-Irradiation-Induced Tissue Damage and Oxidative Stress in BALB/c Mice  

Kang, Jung Ae (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Nam, You Ree (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Rho, Jong Kook (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Jang, Beom-Su (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Chung, Young-Jin (Department of Food and Nutrition, Chungnam National University)
Park, Sang Hyun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.44, no.5, 2015 , pp. 657-663 More about this Journal
Abstract
Ionizing radiation induces cell damage through formation of reactive oxygen species. The present study was designed to evaluate the protective effects of post-treatment with hesperetin against ${\gamma}$-irradiation-induced cellular damage and oxidative stress in BALB/c mice. Healthy female BALB/c mice were exposed to ${\gamma}$-irradiation and administered hesperetin (25 mg/kg and 50 mg/kg, b.w., orally) for 7 days after 6 Gy of ${\gamma}$-irradiation. Exposure to ${\gamma}$-irradiation resulted in hematopoietic system damage manifested as decreases in spleen indexes and WBC count. In addition, hepatocellular damage characterized by increased levels of aspartate aminoransferase (AST) and alanine aminotransferase (ALT) in plasma. However, post-irradiation treatment with hesperetin provided significant protection against hematopoietic system damage and decreased AST and ALT levels in plasma. The results indicate that ${\gamma}$-irradiation induced increases in lipid peroxidation and xanthine oxidase (XO) as well as decreases in antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and glutathione (GSH) in the liver. These effects were also attenuated by post-treatment with hesperetin, which decreased lipid peroxidation and XO as well as increased antioxidant enzymes and GSH. These results show that post-treatment with hesperetin offers protection against ${\gamma}$-irradiation-induced tissue damage and oxidative stress and can be developed as an effective radioprotector during radiotherapy.
Keywords
hesperetin; ${\gamma}$-irradiation; tissue damage; oxidative stress;
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