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http://dx.doi.org/10.3746/jkfn.2014.43.8.1158

Anti-inflammatory Effect of Ethanol Extract from Sargassum fulvellum on Lipopolysaccharide Induced Inflammatory Responses in RAW 264.7 Cells and Mice Ears  

Jeong, Da-Hyun (Department of Food Science & Technology/Institute of Food Science and Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University)
Kim, Min-Ji (Institute of Fisheries Sciences, Pukyong National University)
Kang, Bo-Kyeong (Department of Food Science & Technology/Institute of Food Science and Pukyong National University)
Bark, Si-Woo (Department of Food Science & Technology/Institute of Food Science and Pukyong National University)
Pak, Won-Min (Department of Food Science & Technology/Institute of Food Science and Pukyong National University)
Kim, Bo-Ram (Department of Food Science & Technology/Institute of Food Science and Pukyong National University)
Ahn, Na-Kyung (Department of Food Science & Technology/Institute of Food Science and Pukyong National University)
Choi, Yeon-Uk (Department of Food Science & Technology/Institute of Food Science and Pukyong National University)
Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science and Pukyong National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.8, 2014 , pp. 1158-1165 More about this Journal
Abstract
This study investigated the anti-inflammatory effects of Sargassum fulvellum ethanol extract (SFEE) on the lipopolysaccharide (LPS)-induced inflammatory response. SFEE remarkably suppressed production of NO and pro-inflammatory cytokines (IL-6, $TNF-{\alpha}$, and $IL-1{\beta}$ at 50 and $100{\mu}g/mL$. There were no cytotoxic effects on proliferation of macrophages treated with SFEE compared to the control. SFEE reduced expression of iNOS and COX-2 proteins in a dose-dependent manner. The formation of edema in mouse ears was reduced at the highest dose tested compared to the control. Moreover, in the acute toxicity test, no mortality occurred in mice administered 5,000 mg/kg body weight of SFEE over the 2-week observation period. These results suggest that SFEE may have significant effects on inflammatory factors and be a potential anti-inflammatory therapeutic material.
Keywords
anti-inflammatory effect; $NF-{\kappa}B$; pro-inflammatory cytokines; Sargassum fulvellum;
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