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http://dx.doi.org/10.3746/jkfn.2014.43.3.419

Metabolomic Analysis of Ethyl Acetate and Methanol Extracts of Blueberry  

Jo, Young-Hee (Dept. of Food Science and Technology, Chung-Ang University)
Kim, Sugyeong (Dept. of Food Science and Technology, Chung-Ang University)
Kwon, Da-Ae (Dept. of Food Science and Technology, Chung-Ang University)
Lee, Hong Jin (Dept. of Food Science and Technology, Chung-Ang University)
Choi, Hyung-Kyoon (College of Pharmacy, Chung-Ang University)
Auh, Joong-Hyuck (Dept. of Food Science and Technology, Chung-Ang University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.3, 2014 , pp. 419-424 More about this Journal
Abstract
Metabolite profiling of blueberry (cultivar "Spartan") was performed by extraction using different solvents, methanol and ethyl acetate, through metabolomic analysis using LC-MS/MS. Unsupervised classification method (PCA) and supervised prediction model (OPLS-DA) provided good categorization of metabolites according to the extraction solvents. Metabolites of the anthocyanin family, including delphinidin hexoside, delphinidin, 5-O-feruloylquinic acid, malvidin hexoside, malvidin-3-arabinoside, petunidin-3-arabinoside, and petunidin hexoside, were mainly detected in methanol fractions, whereas those of the flavonoid family, including chlorogenic acid, chlorogenic acid dimer, 6,8-di-C-arabinopyranosyl-luteolin, and luteolin were successfully prepared in the ethyl acetate fraction. Thus, metabolomic analysis of blueberry extracts allows for the simple profiling of whole and distinctive metabolites for future applications.
Keywords
metabolites profiling; blueberry; LC-MS/MS;
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