Browse > Article
http://dx.doi.org/10.3746/jkfn.2014.43.3.389

Antioxidant, Physiological Activities, and Acetylcholinesterase Inhibitory Activity of Portulaca oleracea Extracts with Different Extraction Methods  

Kwon, Yu-Ri (Dept. of Food Science and Technology, Catholic University of Daegu)
Cho, Sung-Mook (Dept. of Food Science and Technology, Catholic University of Daegu)
Hwang, Seung-Pil (Dept. of Food Science and Technology, Catholic University of Daegu)
Kwon, Gi-Man (Dept. of Food Science and Technology, Catholic University of Daegu)
Kim, Jae-Won (Dept. of Food Science and Technology, Catholic University of Daegu)
Youn, Kwang-Sup (Dept. of Food Science and Technology, Catholic University of Daegu)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.3, 2014 , pp. 389-396 More about this Journal
Abstract
The physiological properties of 70% ethanol extracts from Portulaca oleracea with different extraction methods (reflux extraction, RE; autoclave extraction, AE; low temperature high pressure extraction, LTPE) were investigated. The freeze-dried powder yields of RE, AE, and LTPE were 33.78%, 30.80%, and 11.05%, respectively. The color values of L and b were higher in LTPE, and the chroma values were higher in AE and LTPE compared to RE. The total polyphenolics and proanthocyanidin contents in LTPE were significantly higher than in other extracts. The amount of substances related to flavonoids contents was highest in RE (4.30 mg/g), followed by AE (4.06 mg/g), and LTPE (4.00 mg/g). DPPH radical scavenging ability with a concentration of 500 mg% (w/v) were in the following order; LTPE (88.87%)> RE (83.84%)> AE (80.67%). Further, the reducing power, ABTS radical scavenging ability, and nitrite scavenging activity was observed in the same tendency as seen with the DPPH radical scavenging ability. However, the ferrous ion chelating activity of RE (85.45%) and AE (83.88%) was significantly higher than that of LTPE (75.60%). ${\alpha}$-Glucosidase inhibitory activities of RE and LTPE with a concentration of 100 mg% were significantly higher than AE. Xanthine oxidase, and acetylcholinesterase inhibitory activities of LTPE were higher than the other extracts. These results suggest that the extracts from Portulaca oleracea have the potential to act as functional materials, and components of Portulaca oleracea could be effective in the prevention of Alzheimer's disease, and may be used to develop various functional food products.
Keywords
Portulaca oleracea; extraction method; antioxidant activity; acetylcholinesterase inhibitory activity;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Bae JH. 2012. The effect of Portulaca oleracea on the pathogens of gastroenteritis in infants. Korean J Food & Nutr 25: 233-238.   과학기술학회마을   DOI   ScienceOn
2 Rashed AN, Afifi FU, Disi AM. 2003. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1. J Ethnopharmacol 88: 131-136.   DOI   ScienceOn
3 Hung TM, Thung PT, Nhan NT, Mai NTT, Quan TL, Choi JS, Woo MH, Min BS, Bae KH. 2011. Cholinesterase inhibitory activities of alkaloids from Corydalis tuber. Nat Prod Sci 17: 108-112.   과학기술학회마을
4 Yook CS. 1989. Coloured medicinal plants of Korea. Academic Press, Seoul, Korea. p 164.
5 Dewanto V, Wu X, Adom KK, Liu RH. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50: 3010-3014.   DOI   ScienceOn
6 Abdel-Hameed ES. 2009. Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples. Food Chem 114: 1271-1277.   DOI   ScienceOn
7 Danrong Z, Yuqiong C, Dejiang N. 2009. Effect of water quality on the nutritional components and antioxidant activity of green tea extracts. Food Chem 113: 110-114.   DOI   ScienceOn
8 Habtemariam S, Harvey AL, Waterman PG. 1993. The muscle relaxant properties of Portulaca oleracea are associated with high concentrations of potassium ions. J Ethnopharmacol 40: 195-200.   DOI   ScienceOn
9 Xiang L, Xing D, Wang W, Wang R, Ding Y, Du L. 2005. Alkaloids from Portulaca oleracea L. Phytochemistry 66: 2595-2601.   DOI   ScienceOn
10 Park SH, Kim DK, Bae JH. 2011. The antioxidant effect of Portulaca oleracea extracts and its antimicrobial activity on Helicobacter pylori. Korean J Food & Nutr 24: 306-311.   과학기술학회마을   DOI   ScienceOn
11 Bea JH. 1999. Effect of Portulaca oleracea extract on removing nicotine component of tobacco. J Korean Soc Food Sci Nutr 28: 607-612.   과학기술학회마을
12 Won HR, Kim SH. 2011. Antihyperlipidemic effect of diet containing Portulaca oleracea L. ethanol extract in high fat diet-induced obese mice. J Korean Soc Food Sci Nutr 40: 538-543.   과학기술학회마을   DOI   ScienceOn
13 Osawa T. 1994. Novel natural antioxidant for utilization in food and biological system. In Postharvest Biochemistry of Plant Food Material in the Tropics. Uritani I, Garcia VV, Mendoza EM, eds. Japan Scientific Societies Press, Tokyo, Japan. p 241-251.
14 Stirpe F, Della Corte E, Lorenzini E. 1969. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Biol Chem 244: 3855-3863.
15 Ellman GL, Courtney KD, Andres jr V, Featherstone RM. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7: 88-95.   DOI   ScienceOn
16 Sandahl JF, Jenkins JJ. 2002. Pacific steelhead (Oncorhynchus mykiss) exposed to chlorpyrifos: benchmark concentration estimates for acetylcholinesterase inhibition. Environ Toxicol Chem 21: 2452-2458.   DOI
17 Woo JH, Shin SL, Jeong HS, Lee CH. 2010. Influence of applied pressure and heat treatment on antioxidant activities of young leaves from Achillea alpina and Solidago virgaurea subsp. gigantea. Korean J Plant Res 23: 123-130.   과학기술학회마을
18 Gordon MH. 1990. The mechanism of antioxidant action in vitro. In Food Antioxidant. BJF Hudson, ed. Elsevier Applied Food Science Series, London, UK. p 1-18.
19 Saeedeh AD, Asna U. 2007. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem 102: 1233-1240.   DOI   ScienceOn
20 Takashi Y, Yamamoto M, Tamura A. 1978. Studies on the formation of nitrosamines (VII); The effects of some polyphenols on nitrosation of diethylamine. J Food Hyg Soc Jpn 19: 224-229.   DOI
21 Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem 51: 1333-1338.   DOI
22 Yen GC, Duh PD, Tsai HL. 2002. Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid. Food Chem 79: 307-313.   DOI   ScienceOn
23 Baoshan S, Jorge MR, Isabel S. 1998. Critical factors of vanillin assay for catechins and proanthocyanidins. J Agric Food Chem 46: 4267-4274.   DOI   ScienceOn
24 Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.   DOI   ScienceOn
25 Kim KY, Nam KA, Kurihara H, Kim SM. 2008. Potent ${\alpha}$-glucosidase inhibitors purified from the red alga Grateloupia elliptica. Phytochemistry 69: 2820-2825.   DOI   ScienceOn
26 Kim HY, Lim SH, Park YH, Ham HJ, Lee KJ, Park DS, Kim KH, Kim S. 2011. Screening of ${\alpha}$-amylase, ${\alpha}$-glucosidase and lipase inhibitory activity with Gangwon-do wild plants extracts. J Korean Soc Food Sci Nutr 40: 308-315.   과학기술학회마을   DOI   ScienceOn
27 Vincenzo NT. 2001. Acetylcholinesterase in Alzheimer's disease. Mech Ageing Dev 122: 1961-1969.   DOI   ScienceOn
28 Kim JW, Kim JK, Song IS, Kwon ES, Youn KS. 2013. Comparison of antioxidant and physiological properties of Jerusalem artichoke leaves with different extraction processes. J Korean Soc Food Sci Nutr 42: 68-75.   과학기술학회마을   DOI   ScienceOn
29 Berkov S, Bastida J, Nikolova M, Viladomat F, Codina C. 2008. Rapid TLC/GC-MS identification of acetylcholinesterase inhibitors in alkaloid extracts. Phytochem Anal 19: 411-419.   DOI   ScienceOn
30 Kang KM, Lee SH 2013. Effects of extraction methods on the antioxidative activity of Artemisia sp. J Korean Soc Food Sci Nutr 42: 1249-1254.   과학기술학회마을   DOI   ScienceOn
31 Turkmen N, Sari F, Velioglu YS. 2005. The effects of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chem 93: 713-718.   DOI   ScienceOn
32 Jiratanan T, Liu RH. 2004. Antioxidant activity of processed table beets (Beta vulgaris var, conditiva) and green beans (Phaseolus vulgaris L.). J Agric Food Chem 52: 2659-2670.   DOI   ScienceOn
33 Yang Z, Zhang X, Duan D, Song Z, Yang M, Li S. 2009. Modified TLC bioautographic method of screening acetylcholinesterase inhibitors from plant extracts. J Sep Sci 32: 3257-3259.   DOI   ScienceOn
34 Hwang JK, Kim CT, Hong SI, Kim CJ. 1994. Solubilization of plant cell walls by extrusion. J Korean Soc Food Nutr 23: 358-370.   과학기술학회마을
35 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radial cation decolorization assay. Free Radic Biol Med 26: 1231-1237.   DOI   ScienceOn
36 Wagner H, Bladt S, Zgainski EM. 1984. Plant drug analysis: a thin layer chromatography atlas. Springer-Verlag, Berlin, Heidelberg, New York, Tokyo. p 51-90.